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Chemiluminescence immunoassay method for quantitative detection of myeloperoxidase

A technology of chemiluminescence immunity and myeloperoxide, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, and analysis of materials, can solve the problem that the detection sensitivity and linear range cannot meet the requirements of MPO to predict cardiovascular diseases Functional requirements, lack of quantitative functions, etc., to achieve the effect of simple and fast analysis method, long duration of optical signal, and good safety

Inactive Publication Date: 2016-05-25
HANGZHOU JIANKANG BIOTECH CO LTD
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Problems solved by technology

[0003] Therefore, accurate and quantitative detection of MPO is an urgent need in clinical medicine. However, the detection kits used in the market are colloidal gold method, enzyme-linked immunosorbent assay does not have quantitative function, and its detection sensitivity and linear range cannot meet MPO as a prediction Functional requirements in cardiovascular disease

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  • Chemiluminescence immunoassay method for quantitative detection of myeloperoxidase
  • Chemiluminescence immunoassay method for quantitative detection of myeloperoxidase
  • Chemiluminescence immunoassay method for quantitative detection of myeloperoxidase

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[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0020] see figure 1 , in an embodiment of the present invention, a chemiluminescent immunoassay method for the quantitative detection of myeloperoxidase, the kit is composed of an ELISA plate, a standard, a sample diluent, a concentrated washing solution, a reaction solution and a luminescent substrate To detect, specifically include the following steps:

[0021] (1) Preparation: Take out the reagents from the refrigerator at 4°C, and equilibrate at room temp...

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Abstract

The invention discloses a chemiluminescence immunoassay method for quantitative detection of myeloperoxidase. Detection is performed through a kit which is jointly composed of an elisa plate, standard products, sample diluents, concentrated washing liquid, reaction liquid and luminescent substrates. The method specifically comprises the following steps of 1 preparing; 2 sample adding and positioning; 3 sample adding; 4 plate cleaning; 5 substrate adding; 6 luminescent value determining. Accordingly, the sensitivity is high, the linear dynamic range is wide, the optical signal lasting time is long, and the immunoassay method is simple, convenient and rapid, the result is stable and little in error, the safety is good, and the service life is long.

Description

technical field [0001] The invention relates to the field of detection of cardiovascular and cerebrovascular diseases, in particular to a chemiluminescence immunoassay method for quantitative detection of myeloperoxidase. Background technique [0002] Myeloperoxidase (myeloperoxidase, MPO), also known as peroxidase, is the heme protease of the heme prosthetic group, and is one of the members of the heme peroxidase superfamily. , exists in the azurophilic blue granules of myeloid cells (mainly neutrophils and monocytes), and is a specific marker of myeloid cells. With the deepening of MPO research, it has been found that MPO gene polymorphisms lead to individual The difference in susceptibility to some diseases is closely related to the occurrence and development of various human diseases, so it has been paid more and more attention by scholars at home and abroad. Myeloperoxidase (MPO) is an important detection marker for detecting coronary artery disease and acute coronary ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/573
CPCG01N21/76G01N33/573
Inventor 李志华段年丰
Owner HANGZHOU JIANKANG BIOTECH CO LTD
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