The method and application of detection of small copy number variation of goat tmem95 gene by pcr technique

Abstract

The invention discloses a method for detecting goat TMEM95 gene subtle copy number variation through the PCR technology and application thereof. According to the method, goat whole genome DNA, containing the TMEM 95 gene, in a Chinese region serves as a template, a primer pair P1 serves as a primer, part of a sequence of the goat TMEM 95 gene is amplified through PCR, and then a PCR amplification segment is subjected to agarose gel electrophoresis; according to an agarose gel electrophoresis result, non-coding region 58bp subtle copy number variation of the goat TMEM 95 gene is identified; because the TMEM 95 gene has an important regulation function on growth traits and milk production traits, the detection method lays a foundation for building of the relation between the TMEM95 gene subtle copy number variation and production traits, therefore the method can be conveniently used for assisted selection of markers of production traits of goats in China, and goat species with good genetic resources are built easily.

Description

technical field [0001] The invention belongs to the field of modern biotechnology and livestock breeding, and relates to the detection of small copy number variation (CNV), in particular to a method for rapidly and accurately detecting the 58bp small copy number variation (CNV) of goat transmembrane protein 95 (TMEM95) gene. Background technique [0002] Copy number variation (CNV) refers to the increase, decrease, inversion or translocation of the copy number of DNA fragments in the genome caused by genome rearrangement compared with the genome reference sequence. From a narrow point of view, the length of DNA fragments with copy number changes is generally greater than 1kb, but from a broad point of view, the length of DNA fragments with copy number changes is generally greater than 50bp, mainly manifested as submicroscopic deletions and duplications. Copy number variation (CNV) is an important part of genome structural variation (SV), which has the characteristics of wide...

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Problems solved by technology

[0014] At present, there is a lack of research on the genetic variation of the TMEM95 gene in Chinese local goats. The functional research of this gene locus and its genetic variation and production traits (such as weight, body height, body length, chest width, chest circumference, body length index and other growth traits, Milk protein content, total solids content, non-fat milk solids content, lipoprotein density, lactic acidity and other lactation traits, and reproductive traits such as single birth or multiple births) are even blank

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