Tissue culture method for bletillastriata (thunb.) reichb.f. seedlings

Abstract

The invention discloses a tissue culture method for bletillastriata (thunb.) reichb.f. seedlings in the technical field of medicinal plant tissue culture. The method comprises the steps of preparing a germination medium, a subculture medium and a rooting medium; preparing a paper membrane or nonwoven cloth: shearing the paper membrane or the nonwoven cloth into round sheets or square blocks; transferring the germination medium into a culture dish I and tiling the paper membrane or the nonwoven cloth on the surface of the germination medium; transferring the subculture medium into a culture dish II; transferring the rooting medium into a culture dish III; shearing bletillastriata (thunb.) reichb.f. capsules to obtain bletillastriata (thunb.) reichb.f. seeds, uniformly sowing the seeds in the culture dish I, and performing closed culture; clamping the paper membrane or the nonwoven cloth in the culture dish I by using aseptic forceps and directly performing subculture on tissue culture seedlings between the culture dish II and the culture dish III along with the paper membrane or the nonwoven cloth; and hardening and transplanting the seedlings. Compared with the prior art, the method is high in efficiency and small in quantity of work; and the obtained tissue culture seedlings grow well.

Description

technical field [0001] The invention relates to the technical field of tissue culture of medicinal plants, in particular to a method for tissue culture of bletilla seedlings. Background technique [0002] White and ( Bletilla striata (Thunb.) Reichb.f.) is a perennial herbaceous plant belonging to Orchidaceae, also known as "Bletilla striata". Its tuber is a traditional Chinese herbal medicine in my country. It is used in the treatment of tuberculosis, hemoptysis, liver cancer, gastric cancer, duodenal ulcer, internal and external bleeding and other diseases; modern pharmacological research shows that Bletilla striata has obvious inhibitory effect on Mycobacterium tuberculosis, inflammatory cells, etc. , increase the body's immunity and other health effects, and a large amount of superoxide (SOD) synthesized by it also has antibacterial, analgesic and antitumor activities (Zhang Yongwei et al., 2012; Wang Hongying, 2009; Wang Guanghui et al., 2007; Jiangetal.2007). In additi...

AI Technical Summary

Problems solved by technology

However, due to the cumbersome and time-consuming operation of tissue culture technology, it not only increases labor costs, but also increases economic costs, and in the transfer process of seedlings, seedlings are extremely susceptible to bacterial infection and reduce the survival rate of tissue cultured seedlings.

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