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Chuzhou chrysanthemum strain in-vitro regeneration method

A technology of in vitro regeneration and strains, applied in the field of plant biology, can solve the problem of low reproduction coefficient and achieve the effects of high reproduction coefficient, low toxicity and convenient material selection

Active Publication Date: 2016-06-29
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few studies on tissue culture regeneration of Chrysanthemum chrysanthemum, which mainly uses leaves as explants to rapidly propagate chrysanthemum chrysanthemum through indirect induction of callus or through stem tip propagation to cultivate detoxified chrysanthemum chrysanthemum, which generally has a low reproductive coefficient. The problem

Method used

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Effect test

Embodiment 1

[0036] A method for in vitro regeneration of chrysanthemum chrysanthemum strains, the specific operations are as follows:

[0037] 1, select the perennial chrysanthemum chrysanthemum excellent line that grows well from field, cut the non-lignified branch of newborn 20d seedling age;

[0038] 2. After removing the leaves, add 1ml of detergent to a 500ml beaker and wash it in running water for 10 minutes; then cut it into 4cm-sized stems, wash it with sterile water for 3 times in a sterile operating table, and then use 75% alcohol Disinfect for 20 seconds, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric chloride for 1 minute, and finally rinse with sterile water for 6 times. During the disinfection period, keep shaking. After disinfection, cut off the necrotic parts at both ends of the stem, and then cut the stem into sections of about 0.3 cm for later use;

[0039] 3. Plant the cut stems vertically in the adventitious bud induction medium for germinatio...

Embodiment 2

[0044] A method for in vitro regeneration of chrysanthemum chrysanthemum strains, the specific operations are as follows:

[0045] 1. Select the good perennial chrysanthemum chrysanthemum line that grows well from the field, and cut the non-lignified branches of the newborn 30d seedling age;

[0046] 2. After removing the leaves, add 2ml of detergent to a 500ml beaker and wash it in running water for 15 minutes; then cut it into 5cm-sized stems, wash it with sterile water for 3 times in a sterile operating table, and then use 75% alcohol Disinfect for 10 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride for 2 minutes, and finally rinse with sterile water for 7 times, and keep shaking during the disinfection period. After disinfection, cut off the necrotic parts at both ends of the stem, and then cut the stem into sections of about 0.4 cm for later use;

[0047]3. Stem section cuttings are vertically planted in the adventitious bud induc...

Embodiment 3

[0052] A method for in vitro regeneration of chrysanthemum chrysanthemum strains, the specific operations are as follows:

[0053] 1. Select the good perennial chrysanthemum chrysanthemum line that grows well from the field, and cut the non-lignified branches of the new 45d seedling age;

[0054] 2. After removing the leaves, add 2ml of detergent to a 500ml beaker and rinse with running water for 20 minutes; then cut into 6cm-sized stem segments, rinse them twice with sterile water on a sterile operating table, and then use 75% alcohol Disinfect for 30 seconds, rinse with sterile water 4 times, then disinfect with 0.1% mercuric chloride for 3 minutes, and finally rinse with sterile water for 8 times, and keep shaking during the disinfection period. After disinfection, cut off the necrotic parts at both ends of the stem, and then cut the stem into sections of about 0.6 cm for later use;

[0055] 3. Plant the cut stems vertically in the adventitious bud induction medium for ger...

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Abstract

The invention discloses a Chuzhou chrysanthemum strain in-vitro regeneration method. Chuzhou chrysanthemum regenerated strains are obtained by taking newly-growing and non-lignified stem segments, with axillary buds, of field Chuzhou chrysanthemum strains as explants, disinfecting the surfaces of the explants, inoculating the stem segments in an adventitious bud induction medium, conducting illumination cultivation for 1-2 weeks, waiting for stem segment axillary bud germination, conducting induction around the axillary buds to obtain adventitious bud clusters, conducting cultivation for 2-3 weeks, transplanting the stem segments with the adventitious bud clusters to a proliferation medium for proliferation and elongation culture of adventitious buds, transplanting the stem segments to a rooting culture medium for rooting when the buds grow to 2-3 cm, conducting illumination culture for 3-4 weeks, and conducting seedling hardening and transplanting when young seedlings grow to 3-4 cm and have 2-4 main leaves. The method is high in reproduction speed and coefficient, each explant at least generates dozens of cluster buds on average after each time of amplified cultivation, excellent characteristics of Chuzhou chrysanthemum maternal plants are kept, and technical support is provided for the rapid and large-scale reproduction and genetic improvement of excellent Chuzhou chrysanthemum strains.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for in vitro regeneration of chrysanthemum chrysanthemum strains. Background technique [0002] Chuju (Dendranthema morifolium CV.'chuju'), is a perennial herbaceous plant of the genus Chrysanthemum in the Asteraceae family. It is mainly produced in Chuzhou, Anhui, so it is also called Anhui Chuju. Using flowers as medicine is one of the four famous authentic medicinal materials in Anhui Province and ranks first among the four major medicinal chrysanthemums in the country. It is a good product for both medicine and tea, and has extremely high medicinal and health care values. [0003] Modern pharmacological detection and analysis show that Chuju is rich in flavonoids, volatile oil and 8 kinds of essential amino acids for human body; in addition, Chuju is rich in 10 kinds of trace elements such as zinc and selenium, and the content of "selenium" is significa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 侯金艳吴丽芳马朝东
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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