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Tissue culture formula and culture method for lonicera tatarica

A culturing method and technology of formula are applied in the field of tissue culture formulation and cultivation of honeysuckle tartarii, can solve the problems of low reproduction coefficient, no reports and patents on tissue culture of honeysuckle tartarica have been seen, achieve short breeding cycle, meet the needs of landscaping , the effect of short production cycle

Inactive Publication Date: 2016-06-29
NINGXIA HUANGDA BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used propagation methods of Lonicera tartar are seed propagation and cutting propagation, which are limited by the season and have a low reproduction coefficient. At present, there are no reports and patents on the tissue culture of Lonicera tartar.

Method used

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with specific embodiment:

[0038] 1. Preparation of Tatar Honeysuckle Culture Medium Formula

[0039] 1.1 Preparation and storage of medium mother solution

[0040] MS medium contains nearly 30 kinds of nutritional components. In order to avoid weighing these dozens of components every time the medium is prepared, the various components in the medium can be weighed separately according to the integer multiple of the original amount. Into a concentrated solution, this concentrated solution is called the culture medium mother solution.

[0041] Mother liquor ⅠNH 4 NO 3 33g / L, KNO 3 38g / L, MgSO 4 ·7H 2 O7.4g / L, KH 2 PO 4 3.4g / L, mother liquor II CaCl 2 2H 2 O8.8g / L, mother liquor ⅢH 3 BO 3 620mg / L, MnSO 4 ·H 2 O2140mg / L, ZnSO 4 ·7H 2 O860mg / L, KI83mg / L, mother liquor ⅣNa 2 MnO 4 2H 2 O250mg / L, CuSO 4 ·5H 2 O25mg / L, CoCl 2 ·6H 2 O25mg / L, mother liquor ⅤNa 2 ·EDTA3.73g / L, FeSO 4 ·7...

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Abstract

The tissue culture formula and culture method of honeysuckle tartara of the present invention obtain a sterile culture system by sterilizing the explants, and then use the ratio of different types and different concentrations of exogenous hormones to subculture and grow the tissue culture seedlings Screening and regulation of rooting and rooting, and finally determine the best explant and medium formula, so that it can adapt to factory production. The tissue culture formula and method can reproduce honeysuckle tartar in large quantities to meet the needs of landscaping, and the produced seedlings have stable shape, short production cycle, and can meet large-scale rapid production. The invention can rapidly proliferate without being restricted by seasons, has a short breeding cycle, can completely inherit the excellent characteristics of the female parent, and solves a difficult problem for the industrialized production of tissue culture.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a tissue culture formula and a culture method of Lonicera tartare. Background technique [0002] Tartary honeysuckle, also known as peach-colored honeysuckle and Xinjiang honeysuckle, is a deciduous shrub in the Lonicera family Lonicera, up to 3 meters high. Leaves ovate or ovate-elliptic, 2-6 cm long. The flowers are axillary in pairs, the total pedicel is 1-2 cm long, the corolla is lip-shaped, pink, red or white, smooth outside and hairy inside. Berries red, often conjoined. Tartary honeysuckle blooms luxuriantly and brilliantly, the white ones are pure and noble, the red ones are passionate like fire, and the pink ones are delicate and charming, each color is unique! In summer, the red fruit is as bright as agate, round as a pearl, and the branches are full of fire, so it has high garden value. [0003] At present, the commonly used propagation methods of honeys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李根生
Owner NINGXIA HUANGDA BIOLOGICAL TECH CO LTD
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