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Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva

A technology of Hemerocallis grandiflorum group and Hemerocallis grandiflorum, which is applied in the field of direct rooting of hemerocallis grandiflora tissue culture cluster seedlings, can solve the problems of low seed setting rate and low reproduction rate, and achieve the effect of meeting demand, high reproduction multiple, and accelerated reproduction speed

Inactive Publication Date: 2013-12-25
TANGSHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to cultivation observation, some large-flowered varieties have low seed-setting rate and can only be propagated by ramets, and the annual multiplication rate is only 2-3 times, and the reproduction rate is low, which is far from meeting the large demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Example: direct rooting and transplanting of clustered seedlings of Hemerocallis grandiflorum variety "Hongyun".

[0023] The young flower stalks of Hemerocallis grandiflora "Hongyun" were explants to induce callus tissue, and clustered buds were produced by differentiation medium. The cut pieces of callus with clustered shoots were transferred to MS medium without plant growth regulators to form strong shoots to form neat and strong clustered shoots, and the clustered shoots began to root during this process.

[0024] After the clustered seedlings take root, they can be transplanted when the roots grow to 1 cm. During the period from February to April this year, a total of 4 times were transplanted, and 4740 bushy root seedlings were transplanted in transition, with a survival rate of over 98%. After 20-30 days of vermiculite transition, each single plant of the clustered seedlings has new roots, and it is easy to separate, and each cluster can produce 3-8 independent...

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Abstract

The invention discloses a direct rooting method for tissue culture cluster seedlings of hemerocallis fulva. The method comprises the steps of: inducing a hemerocallis fulva explant on a culture medium to produce callus, culturing cluster buds on a differential medium, slicing the callus, grafting the sliced callus on the culture medium for strong seedling culture; transitionally cultivating the cluster seedlings; in the transitionally cultivating process, planting the rooted cluster seedlings in a cultivation tank taking vermiculite as matrix, covering the cultivation tank with a mulching film; within the following 10 days, gradually reducing the humidity in the humidity tank to the normal humidity of a greenhouse by drilling the mulching film, uncovering the mulching film, and spraying a nutrient solution on leaf surfaces at the same time. The direct rooting method for tissue culture cluster seedlings of hemerocallis fulva disclosed in the invention, used for culturing cluster, is not only high in propagation multiples, but also not limited by seasons; so the method can accelerate the propagation and satisfy the requirement of garden afforestation; and meanwhile, the method provided by the invention simplifies the operation process of tissue culture, reduces the cost and increases the transplantation efficiency and survival rate of the tissue culture seedlings.

Description

technical field [0001] The invention relates to a rooting method for tissue-cultured clustered seedlings of ornamental plants, in particular to a method for directly rooting the tissue-cultured clustered seedlings of Hemerocallis grandis, which is suitable for various varieties of Hemerocallis grandis. Background technique [0002] Hemerocallis hybrida hort. is a perennial herbaceous perennial flower of the Liliaceae Hemerocallis genus, which has strong drought resistance, cold resistance, salt resistance, and pest resistance. It can be planted in a large area around the lawn or in the center of the lawn in groups to increase the three-dimensional effect. It is a rare new variety of perennial flowers in landscaping. Through cultivation observation, some large-flowered varieties have low seed setting rate, and can only be propagated by division, and the annual multiplication rate is only 2-3 times, and the reproduction rate is low, which is far from meeting the large demand o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 陈超王桂兰张红心马云霄孙献明
Owner TANGSHAN NORMAL UNIV
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