Magnetic bead separation device for nucleic acid extraction

Abstract

The invention provides a magnetic bead separation device for nucleic acid extraction.The magnetic bead separation device comprises a separation head lifting module, a magnetic rod module, a stirring module, an isolation sleeve separation module, isolation sleeves and reaction consumables, wherein the magnetic rod module and the stirring module are respectively in sliding connection with the separation head lifting module, the magnetic rod module comprises a plurality of magnetic rods capable of vertically moving, the stirring module comprises a plurality of gear shafts corresponding to the magnetic rods, the adjacent gear shafts are connected through gears, the core portions of the gear shafts are hollow, the lower portion of the gear shafts are used for sleeved connection with the isolation sleeves, the isolation sleeve separation module is fixed to the stirring module and is used for taking off the isolation sleeves from the gear shafts, and the reaction consumables are arranged under the gear shafts and include a splitting decomposition container, a cleaning container and an elution container.The magnetic rods are permanent magnets, and the permanent magnets are relatively small and exquisite in structure.An even liquid mixing mode of the magnetic bead separation device is a rotary-stirring even-mixing mode, the rotating speed is adjustable, and the even mixing consistency is good.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a magnetic bead separation device for nucleic acid extraction. Background technique [0002] Nucleic acid includes two kinds of molecules, DNA and RNA. Biotechnology with nucleic acid as the research object, including a series of technologies such as extraction, cloning, amplification, detection, and sequencing of nucleic acid, has developed rapidly in recent years. These technologies are currently not only used in research units , and has been widely used in multiple functional departments in social life. The first step in nucleic acid research is to extract nucleic acid from various biological samples, and the efficiency of nucleic acid extraction becomes a restrictive factor for the success of downstream nucleic acid research. Nucleic acid exists in the state of binding to protein in cells. The main steps of nucleic acid extraction are: lysing cells to remove protein bound t...

AI Technical Summary

Problems solved by technology

[0004] The common manual nucleic acid extraction uses a vibrator to mix, and the common automatic nucleic acid extraction device uses the up and down vibration method, and the consistency of the up and down vibration mixing is worse than that of the rotary stirring

Moreover, existing nucleic acid extraction devices usually use electromagnets to absorb and transfer magnetic beads, and the electromagnet needs to be connected to a circuit, and the coil of the electromagnet is very bulky

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