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Tissue culture method of succulent giant red line ho1

A technology for giant succulents and succulents, applied in the field of tissue culture of succulents HO1 giant succulents, can solve the problems of affecting HO1 reproduction, affecting economic benefits, slow reproduction speed, etc., to improve transplanting survival rate and high proliferation coefficient, the effect of fast provision

Active Publication Date: 2018-02-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the traditional method of leaf cutting and seed sowing is used for cultivation, not only the propagation speed is extremely slow, only 1-2 leaves are grown in a year, but also the survival rate is low, which seriously affects the reproduction of HO1, thereby affecting its economic benefits.

Method used

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  • Tissue culture method of succulent giant red line ho1
  • Tissue culture method of succulent giant red line ho1
  • Tissue culture method of succulent giant red line ho1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: A tissue culture method of succulent plant giant red line HO1, the following steps are carried out in sequence:

[0069] 1) Take the pedicel of the giant red line HO1 plant that grows robustly and is free from diseases and insect pests, put it into a gauze bag and wash it with tap water for 1 to 2 hours;

[0070] 2), callus induction:

[0071] After the pedicels after the above-mentioned flowing water washing are routinely disinfected (that is, 0.1% w / v HgCl 2 After 6 to 8 minutes of treatment, rinse with sterile water 5 to 6 times, then blot dry with sterile filter paper), cut into 3 to 5 mm in size, and inoculate it on the culture medium for inducing callus; the culture condition is: 16 hours Light, light intensity 30 ~ 40μmol m -2 ·s -1 , the temperature is (25±1)°C; 8 hours of dark cultivation, the temperature is (21±1)°C; the above-mentioned light and dark cultivation are carried out alternately;

[0072] Callus induction medium: MS+6-BA2mg / L+KT1m...

Embodiment 2

[0091] Compared with Example 1, 0.1% activated carbon was added on the medium used in steps 4) to 6), namely:

[0092] Proliferation medium: MS+6-BA0.15mg / L+NAA0.05mg / L+1g / L activated carbon+white sugar 25g / L+agar 8g / L, pH 5.6.

[0093] The rooting medium is: 1 / 2MS basic medium + 1g / L activated carbon + white sugar 25g / L + agar 8g / L, pH is 5.6.

[0094]Strong seedling medium: 1 / 2MS basic medium + 0.1mg / LNAA + 1g / L activated carbon + white sugar 25g / L + agar 8g / L, pH 5.6.

[0095] The rest are equal to Example 1.

[0096] The result is:

[0097] No obvious browning phenomenon was seen during the culture process of leaf induced adventitious buds in step 4).

[0098] In the rooting culture of step 5), the statistical rooting rate was 90% in 24 days.

[0099] This embodiment 2 is compared with embodiment 1, has increased 0.1% gac on the substratum of multiplication (subculture), rooting and strong seedling. Activated carbon can absorb harmful substances produced by plants, pr...

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Abstract

The invention discloses a tissue culture method of succulent Haworthia emelyae v.comptoniana 'KYODAI AKASEN', comprising the following steps: washing and sterilizing pedicels of Haworthia emelyae v.comptoniana 'KYODAI AKASEN' HO1 growing vigor and free of disease and insect damage, cutting the pedicels into segments, and transversely placing and inoculating the segments to a callus culture medium for inducing calluses; when sterile calluses grow in a cluster form, inoculating small calluses to adventitious buds inducing medium for inducing adventitious buds; when sterile seedlings induced by the above-mentioned calluses grow to 0.6-1.5 cm in diameter, cutting leaves and inoculating the leaves to a multiplication medium for carrying out adventitious bud culture by leaf induction; subjecting adventitious buds induced with the leaves to rooting culture, strong seedling culture and transplanting.A large number of tissue culture seedlings of Haworthia emelyae v.comptoniana 'KYODAI AKASEN' HO1 can be obtained using the method.

Description

technical field [0001] The invention relates to a tissue culture method of succulent giant red line HO1. Tissue culture rapid propagation method of giant red line HO1. Background technique [0002] The succulent plant Huge Chixian HO1 is a species of Yulu in the genus Twelve Volumes of the family Agaveaceae. The plant is exquisite and small, the adult window is 2.5cm, the leaf color is crystal clear, the leaves are fluffy, and the edges and corners are obvious. It can be used as a handicraft in life. It is very cute. It is one of the more popular small succulent plant varieties in recent years. Collectors' favorite. However, when the traditional method of leaf cutting and seed sowing is used for cultivation, not only the propagation speed is extremely slow, only 1-2 leaves are grown in a year, but also the survival rate is low, which seriously affects the reproduction of HO1, and then affects its economic benefits. Contents of the invention [0003] The technical proble...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 毛碧增马阳阳龚莺张亚惠
Owner ZHEJIANG UNIV
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