Application of PCDH18 gene to preparation of colorectal cancer diagnosis kit and kit

A 1. PCDH18, diagnostic kit technology, applied in biological testing, biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of no research revealing the relationship between colorectal cancer development, etc., to improve 5-year survival rate, broad application prospects, and timely diagnosis

Active Publication Date: 2016-07-13
XIAMEN INST OF RARE EARTH MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing studies have shown that PCDH18 can activate memory T cells, but no studies have revealed its relationship with the development of colorectal cancer

Method used

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  • Application of PCDH18 gene to preparation of colorectal cancer diagnosis kit and kit
  • Application of PCDH18 gene to preparation of colorectal cancer diagnosis kit and kit
  • Application of PCDH18 gene to preparation of colorectal cancer diagnosis kit and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Fluorescent quantitative PCR method to detect the differential expression of PCDH18 gene in colorectal cancer tissue and corresponding distant normal intestinal tissue

[0048] 1. Collect 25 pairs of colorectal cancer tissue and corresponding distant normal intestinal tissue samples. All colorectal cancer tissue samples are provided by the Specimen Bank of the First Affiliated Hospital of Xiamen University. Each patient sample has a clear diagnosis record, and passed With the approval of the ethics committee, the specimens were collected and frozen in liquid nitrogen.

[0049] 2. Preparation of RNA samples

[0050] (1) Pretreatment of the sample: Take the sample out of the liquid nitrogen tank, put the sample into a 2ml sterile EP tube, add 1ml TrizolRNA extraction solution, soak it in 0.1% DEPC water overnight, cut it into pieces with sterilized scissors, and put it into the tissue Grind thoroughly in a homogenizer.

[0051] (2) Extraction: add 500 μL of c...

Embodiment 2

[0070] Example 2: Fluorescent quantitative PCR method to detect the differential expression of PCDH18 gene in colorectal cancer cell lines and normal intestinal epithelial cell lines

[0071] 1. Cultivate human colorectal cancer cell lines (SW480, SW620, HCT116 and HT29) and human normal intestinal epithelial cell line NCM460. Mycin each 100U / mL in DMEM medium, at 37°C and 5% CO 2 Cultured in an incubator, the culture medium was changed every 2-3 days and subcultured. Human intestinal cancer SW480 uses L15 medium (containing 10% fetal bovine serum), and human intestinal cancer HCT116, SW620 and HT29 cell lines adopts DMEM medium (containing 10% fetal bovine serum). 2 cultured in an incubator.

[0072] 2. When the confluence of the cells in the 60mm culture dish reaches 80%, discard the culture medium, add 1mL sterile PBS to wash, discard the PBS and repeat once, add 1mL TrizolRNA extraction solution, and gently shake the culture dish left and right to make The TrizolRNA ext...

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Abstract

The invention provides application of a colorectal cancer diagnosis molecular marker, namely, a PCDH18 gene which is good in specificity and sensitivity in preparing a colorectal cancer diagnosis kit, and the colorectal cancer diagnosis kit. The application has the main benefits that a novel molecular marker of colorectal cancer is discovered, application of the PCDH18 gene to preparation of the colorectal cancer diagnosis kit and the colorectal cancer diagnosis kit are provided, and compared with a conventional detection method, the molecular marker is relatively timely and specific in diagnosis, so that the five-year survival rate of patients suffering from colorectal cancer can be increased, the death rate is reduced, and wide application prospects can be achieved.

Description

(1) Technical field [0001] The invention relates to the application of PCDH18 gene in preparing a colorectal cancer diagnostic kit, and a colorectal cancer diagnostic kit. (2) Background technology [0002] Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive tract. In the world, the incidence rate ranks third among malignant tumors, and the death rate ranks fourth. There are about 1.2 million new cases every year. Threat to human health. Colorectal cancer has obvious regional distribution differences in the world, and the incidence rate is higher in Asia, especially in China, which is mainly related to risk factors such as age, family history, unhealthy diet and living habits. Most colorectal cancer patients are already in the advanced stage of tumor when they are diagnosed, and have lost the best opportunity for treatment. As a result, the five-year survival rate is less than 20%, and the prognosis is poor. Therefore, exploring the evaluati...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/158C12Q2600/166G01N33/57419G01N33/57484G01N33/68G01N2333/47
Inventor 张云周丹安汉祥汤玮玮
Owner XIAMEN INST OF RARE EARTH MATERIALS
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