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A method for improving the survival rate of melaleuca spore transplanting
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A transplanting survival rate, Melaleuca tower technology, applied in the biological field, can solve problems such as slow progress, and achieve the effect of improving transplanting efficiency
Inactive Publication Date: 2017-11-28
NORTHWEST UNIV
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Problems solved by technology
People try to use tissue culture technology or cuttings to quickly propagate Melaleuca, but progress is slow
Method used
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Embodiment 1
[0036] The spores of Melaleuca spores were transplanted into the moss substrate fully moistened with tap water, and then placed in the culture room for cultivation. The culture conditions are as follows: the humidity is greater than 75%, the temperature is 25° C., the light intensity is 2000 Lux, and the photoperiod is 8 hours. During the cultivation process, water with tap water to keep the moss moist. After 60 days, the transplanting survival rate of Melaleuca spores was 32%.
Embodiment 2
[0038] The spores of Melaleuca spores were transplanted into the moss substrate sprayed with MS culture solution containing 3mg / L indolebutyric acid, and then placed in the culture room for cultivation. The culture conditions are as follows: the humidity is greater than 75%, the temperature is 25° C., the light intensity is 2000 Lux, and the photoperiod is 8 hours. During the cultivation process, water with tap water to keep the moss moist. After 60 days, the transplanting survival rate of Melaleuca spores was 46%.
Embodiment 3
[0040] Step 1, obtaining the prothallus of the associated fern in the Melaleuca tower:
[0041] Take the leaves of Melaleuca, and use the conventional plant explant sterilization method to carry out surface sterilization, then inoculate the surface-sterilized Melaleuca leaves on MS solid medium, and place them in the culture room for cultivation. The culture conditions are: : The temperature is 28°C, the light intensity is 1000 Lux, and the photoperiod is 12 hours. After culturing for 2 months, the prothallus of the associated fern grew from the edge of the leaves of Melaleuca.
[0042] Step 2, the acquisition of the moss substrate for cultivating Melaleuca spores:
[0043] When the prothallus grows to 0.5cm 3 Above, it was transferred to the moss substrate for cultivation. The culture conditions are as follows: the humidity is greater than 75%, the temperature is 25° C., the light intensity is 2000 Lux, and the photoperiod is 8 hours. During the cultivation process, water...
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Abstract
The invention discloses a method for improving the survival rate of the spores of Melaleuca spores after transplanting. The method comprises the steps of using the explants of the Melaleuca plant to obtain the prothallus of the associated fern of the Melaleuca, and transferring the prothallus of the associated fern of the Melaleuca Cultivate in the moss substrate, and then use the moss substrate that has cultivated the prothallus of the associated fern of the Melaleuca to carry out the transplanting culture of the spores of the Melaleuca; The spores of the layered tower improve the transplanting survival rate of the spores of the layered tower. The invention is unique and easy to implement, and provides an important way for the artificial propagation of Melaleuca towers.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the survival rate of Huperzia serrata (Thunb.) Trev. spore transplantation. Background technique [0002] Melaleuca (Huperzia serrata (Thunb.) Trev.) is a perennial herbaceous fern belonging to Lycopodiaceae (Lycopodiaceae) of the order Lycopodiales. It has the effects of dissipating blood stasis, reducing swelling, detoxifying and relieving pain. In 1927, Chinese scientific and technological workers reported that huperzine A isolated from this plant has the effect of relaxing striated muscles. Afterwards, a large number of studies found that huperzine A is a highly efficient, low toxicity, reversible and highly selective inhibitor of acetylcholinesterase. agent. Now, it is used as a drug for treating Alzheimer's disease (AD, Alzheimer disease) by huperzine A, and it is also used as a nutraceutical for intelligence in the United States. [0003] ...
Claims
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Application Information
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