In-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs

A technology for plants and bulbs of the genus Lycoris, applied in the field of isolated primary bulb meristems, can solve the problems of long induction period, low induction rate, waste, etc., and achieve the effects of high degree of developmental synchronization, reduced pollution rate, and consistent traits

Inactive Publication Date: 2016-08-10
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the tissue culture and propagation systems of Lycoris plants all use mature bulbs as explants to realize plant regeneration, mostly in the form of bulb blocks or double scales. There are three main defects: one is that mature bulbs live in the natural soil environment for a long time , carry more bacteria, fungi,

Method used

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  • In-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs
  • In-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Huajinhua selfing primary bulb meristem method

[0035] (1) Germination and cultivation of seeds: On the ultra-clean workbench, take the sterilized natural self-bred seeds of Lycoris sprengeri and inoculate them. The method is to clamp the seeds with clean tweezers and insert the seed hole downwards. medium, the seeds are fully contacted with the medium, and after 30 days of cultivation, the germination rate of the seeds reaches 89.7%. -2 ·s -1 , light time 14h·d -1, carried out under tissue culture conditions at a temperature of (25±2)°C. The germination medium used for inoculation uses MS medium as the basic medium, and also contains 0.2mg / L α-naphthaleneacetic acid, 1.0mg / L 6-benzylaminoadenine, 30g / L sucrose, 8g / L L agar, the pH of the seed germination medium was 5.8.

[0036] (2) Proliferation and cultivation of primary bulbs: after 50 days of seed germination, clamp the bulbs with clean tweezers on the ultra-clean workbench, and inoculate them on ...

Embodiment 2

[0042] The original bulb meristem method of the hybrid of embodiment 2 changing brocade flower and long tube Lycoris

[0043] (1) Germination and cultivation of hybrid seeds: On the ultra-clean workbench, take sterilized hybrid seeds of Lycoris sprengeri and Lycoris longituba and inoculate them with clean tweezers. Seeds are inserted into the culture medium with the seed hole facing down, so that the seeds are fully contacted with the culture medium. After 20 days of cultivation, the germination rate of the seeds reaches 78.3%. -2 ·s -1 , light time 14h·d -1 , carried out under tissue culture conditions at a temperature of (25±2)°C. The germination medium that is used for inoculation takes MS medium as basic medium, and also contains the 6-benzylaminoadenine, 30g / L of sucrose, 8g / L agar, and the pH value of the seed germination medium is 5.8.

[0044] (2) Proliferation and cultivation of primary bulbs: after 45 days of seed germination, clamp the bulbs with clean tweezers...

Embodiment 3

[0049] The original bulb meristem method of the hybrid of embodiment 3 red and blue lycoris and changing Jinhua

[0050] (1) Germination and cultivation of hybrid seeds: On the ultra-clean workbench, take the sterilized hybrid seeds of Lycoris haywardii and Lycoris sprengeri and inoculate them with clean tweezers. Seeds are inserted into the culture medium with the seed hole facing down, and the seeds are fully contacted with the culture medium. After 25 days of cultivation, the seed germination rate reaches 83.3%. -2 ·s -1 , light time 14h·d -1 , carried out under tissue culture conditions at a temperature of (25±2)°C. The germination medium used for inoculation uses MS medium as the basic medium, and also contains 0.2mg / L α-naphthaleneacetic acid, 1.0mg / L 6-benzylaminoadenine, 30g / L sucrose, 8g / L L agar, the pH of the seed germination medium was 5.8.

[0051] (2) Proliferation and cultivation of primary bulbs: After 60 days of seed germination, clamp the bulbs with clean...

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Abstract

The invention relates to an in-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs, which belongs to the technical field of rapid proliferation of plants. The method comprises the following steps: germination culture of seeds; proliferation culture of probulbs; and swelling culture of adventitious buds until substantially-swollen small bulbs are obtained. According to the invention, the probulbs obtained after seed germination are used as explants, and proliferation culture and swelling culture are carried out through basal disc cutting of in-vitro bulbs; so the rate of pollution is substantially reduced; loss of wild seedball resources of the Lycoris herbs is decreased; and a great number germplasm populations with a high growth synchronization degree and consistent properties can be efficiently obtained in a short period.

Description

technical field [0001] The invention relates to the technical field of rapid plant propagation, in particular to an isolated primary bulb meristem method for rapid propagation of Lycoris plants. Background technique [0002] Long vegetative growth period and low natural reproduction coefficient are two important problems restricting the large-scale production and breeding of Lycoris in my country. Under natural conditions, the seeds sown in the matrix usually go through the dormancy period in winter, and can only germinate in the spring of the following year. In the first growth cycle, only 1 to 2 leaves grow, and generally do not accumulate enough nutrients before turning into mature flowering bulbs. Make a natural split. [0003] However, under tissue culture conditions, seed dormancy can be broken by cold storage and soaking in warm water before inoculation. At the same time, through inoculation under the culture conditions of different ratios of hormones, the nutrient ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 夏宜平任梓铭
Owner ZHEJIANG UNIV
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