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Method for proliferation of mink canine distemper virus by applying novel cell microcarrier

A technology of cell microcarrier and canine distemper virus, which is applied in the field of proliferation, can solve the problems of large batch-to-batch variation, low antigen content, and low production efficiency, and achieve the effects of reducing production costs, high cell density, and high production efficiency

Inactive Publication Date: 2016-08-10
SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY
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Problems solved by technology

[0002] The traditional rotary bottle production process has many shortcomings such as large batch-to-batch variation, low antigen content and low production efficiency.

Method used

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  • Method for proliferation of mink canine distemper virus by applying novel cell microcarrier

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Embodiment Construction

[0020] The present invention will be further described below:

[0021] In Example 1, when the microcarrier is used in cell culture, the cell culture container need not be pre-siliconized, and the Flask cell culture bottle and the 3L spinner bottle are used to gradually enlarge and cultivate Vero or DF1 cells. When the spinner bottle cell quantity reaches When required, aseptically collect spinner bottle cells as seed cells.

[0022] b) Selection and preparation of microcarriers for cell culture: selection new type Cephodex cell culture microcarriers are carriers for cell-attached growth. According to the dosage of 10g / L, a certain amount of Cephodex microcarriers was weighed according to the culture volume and put into PBS, and fully stirred with a glass rod to disperse and suspend the microcarriers in PBS.

[0023] c) Bioreactor preparation: Inject the suspended microcarriers into the reactor. After the installation is complete, put the tank body at 121°C for 30 minutes to...

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Abstract

The invention discloses a method for proliferation of a mink canine distemper virus by applying a novel cell microcarrier. The method realizes efficient proliferation of the mink canine distemper virus by applying the novel cell microcarrier and a large-scale high-density cell suspension culture technology, has advantages of large cell density, high virus titer, high production efficiency, strong process controllability, uniform and stable product quality and the like, and overcomes a plurality of defects of batch-batch difference, long antigen content, low production efficiency and the like in products produced according to the traditional spinner bottle production technology.

Description

technical field [0001] The invention relates to a multiplication method, in particular to a method for multiplication of mink canine distemper virus using novel cell microcarriers. Background technique [0002] The traditional spinner bottle production process has many shortcomings such as large batch-to-batch variation, low antigen content and low production efficiency. At the same time, compared with traditional microcarrier products, the new cell culture microcarrier used in the present invention has a very simple method of use, stable and reliable cell culture performance, easy process amplification, greatly improved work efficiency and reduced production costs. Contents of the invention [0003] The purpose of the present invention is just to provide a kind of method that uses novel cell microcarrier to proliferate mink canine distemper virus in order to solve the above problems. [0004] The present invention achieves the above object through the following technical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N5/07
CPCC12N7/00C12N5/0075C12N2531/00C12N2760/18451
Inventor 庄金秋梅建国沈志强
Owner SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY
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