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Dendrobium officinale seed production method based on flower organ development and corresponding obtaining method

A technology for Dendrobium officinale and flower organs is applied in the field of Dendrobium officinale seed production, which can solve the problems of inconsistent seed quality, weak theoretical foundation, and irregular seed production, achieve consistent genetic background, solve production bottlenecks, and improve fruit setting. rate effect

Active Publication Date: 2018-04-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the theoretical basis of breeding research on Dendrobium candidum in my country is weak, and the seed production is not standardized
Many enterprises directly pollinate Dendrobium officinale with different genetic backgrounds such as different varieties, strains and sources for seed production, resulting in mixed varieties, inconsistent species, and inconsistent quality.

Method used

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  • Dendrobium officinale seed production method based on flower organ development and corresponding obtaining method
  • Dendrobium officinale seed production method based on flower organ development and corresponding obtaining method
  • Dendrobium officinale seed production method based on flower organ development and corresponding obtaining method

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Embodiment 1

[0052] Embodiment 1, a kind of method that obtains the Dendrobium officinale seed production method based on flower organ development, carries out following steps successively:

[0053] 1), hang a label (that is, make a mark) at the base of the branch of the first flower bud;

[0054] Carry out the following operations respectively with the two branches listed, that is, one of the branches carries out the following step 2), and at the same time, the other branch carries out the following step 3);

[0055] 2) Take pictures of the branch every day or record the change of the entire flowering period of each small flower in the entire raceme from the beginning of bud formation to the end of flower withering, and indicate the flowering date on the label;

[0056] 3), the branch picks florets with different flowering days in the same raceme throughout the flowering period; carefully take out 2 pieces of pollen in the small anther cap of Dendrobium officinale, put them in a centrifug...

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Abstract

The invention discloses a dendrobium officinale seed preparation method based on development of flower organs. The dendrobium officinale seed preparation method comprises the following steps: pollinating stigmas of flowers blossoming for 8-21 days with anther of flowers blossoming for 8-14 days. The invention further provides a method for obtaining the dendrobium officinale seed preparation method, and the method comprises the following steps: (1) when dendrobium officinale branches have flower buds, hanging tags; (2) picking small flowers with different blossoming days of a whole flowering phase in the same raceme; (3) determining the activity of anther of the small flowers with the different blossoming days of the whole flowering phase by adopting a TTC dyeing method; (4) determining the pollination capability of stigma of the small flowers with the different blossoming days of the whole flowering phase by adopting a benzidine-hydrogen peroxide method; and (5) in the same raceme, pollinating stigmas with strong pollination capability with anther with strong anther activity. According to the method, capsules with consistent genetic background and high fruit bearing rate can be obtained after about 150 days.

Description

technical field [0001] The invention relates to a seed production method of dendrobium officinale based on the development of floral organs. Background technique [0002] Dendrobium officinale Kimura et Migo is a traditional precious Chinese herbal medicine in my country, which has unique effects such as nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. As early as the Qin and Han Dynasties, "Shen Nong's Materia Medica" recorded that Dendrobium candidum "mainly injures, eliminates numbness, lowers qi, nourishes the five viscera, strengthens yin, and thickens the stomach after long-term use"; "Tao Zang" more than 1,000 years ago put the iron skin Dendrobium is listed as the first of the "Nine Great Immortal Herbs in China"; Li Shizhen commented in "Compendium of Materia Medica" that Dendrobium candidum "strengthens yin and nourishes essence, thickens the stomach, nourishes internal deficiency, calms stomach qi, grows muscles, improves int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 毛碧增张亚惠娄沂春
Owner ZHEJIANG UNIV
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