Anti-tumor drug and use thereof

A technology of drugs and compositions, applied in antitumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as limited application and teratogenicity of cyclopamine, and achieve the effect of reducing dosage

Inactive Publication Date: 2016-08-24
RUIAN PULUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the risk of teratogenicity of cyclopamine, its application in medicine is limited. How to avoid the potential risk of cyclopamine is a difficult problem in the development of drugs similar to cyclopamine.

Method used

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  • Anti-tumor drug and use thereof
  • Anti-tumor drug and use thereof
  • Anti-tumor drug and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Detection of Cancer Cell Growth Inhibitory Activity

[0033] MTT micro-cultivation experiment: Prepare the medium containing the drug composition to be tested by two-fold dilution method, incubate the cells with the drug-containing medium, perform MTT rapid colorimetry at 24 hours and 48 hours of culture respectively, and perform on a microplate reader Absorbance (A) values ​​were measured at a wavelength of 490 nm.

[0034] Inhibition rate=[(A value of control group-A value of experimental group) / A value of control group]×100%.

[0035] The regression equation is obtained from the concentration-inhibition rate curve to obtain 50% inhibitory concentration (IC50), 10% inhibitory concentration (IC10), maximum inhibitory rate or maximum inhibitory concentration.

[0036] The effects of each group of pharmaceutical compositions on BCG-823 and CAOV3 were detected by MTT colorimetry, and the time of action on BCG-823 and CAOV3 was 24 hours. From the concentration-i...

Embodiment 2

[0046] Example 2 Colony formation experiment

[0047] to 25cm 2 Add about 200 cell suspensions of BCG-823 or CAOV3 to the culture flask, and after microscopic counting, add 5ml of different drug-containing media respectively. The final concentration of cyclopamine in the media is 10 μM / L. After culturing for one week, Count the number of colonies.

[0048] Colony formation rate R=(number of colonies / number of implanted cells)×100%.

[0049]The test results are shown in Table 2.

[0050] Table 2 Effect of pharmaceutical composition on BCG-823 and CAOV3 colony formation

[0051]

[0052] In the control group without any drug, the R value of BCG-823 cells was 84.6%; the R value of CAOV3 cells was 62.8%.

[0053] It can be seen from the above results that the pharmaceutical composition of the present invention can significantly inhibit the colony formation of tumor cells.

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Abstract

The invention discloses an anti-tumor drug and a use thereof. The invention concretely discloses a pharmaceutical composition for reducing a cyclopamine teratogenic risk. The pharmaceutical composition contains one or more of gentiopicroside, loganic acid and sophocarpidine. The pharmaceutical composition substantially inhibits tumor cell growth activity and greatly reduces a cyclopamine use amount.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an antitumor drug and its application. Background technique [0002] Cyclopamine, also known as cyclopamine and deoxymustamine, is a heterosteroidal alkaloid isolated from Veratrum, mainly found in Veratrum californicum in Liliaceae. ), Indian deer herbivore (Cornlily), Veratrum grandiflorum (Veratrum grandiflorum), and Ibe (Fritillaria pallidiflora Schrenk) can bind to the Smoothened (Smo) protein in the Hedgehog signaling pathway, thereby inhibiting the protein activity. It was discovered in the 1960s because of its teratogenic effect, but studies after the 1990s showed that cyclopamine is a hedgehog signaling pathway inhibitor, which has been confirmed in Drosophila, due to the hedgehog signaling pathway Mutations are associated with the pathogenesis of various tumors. Recent studies have found that cyclopamine has anti-tumor effects in adults, and it has been confirmed in vivo or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61K31/7048A61P35/00A61K31/4375
Inventor 杨钟华
Owner RUIAN PULUO BIOTECH
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