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Preparation and application of echovirus type 9 vp1 protein specific antigenic epitope and its fusion protein

A technology of fusion protein and antigenic epitope, which is applied in the field of genetic engineering technology, vaccines and diagnostic reagents, and can solve the problems of time-consuming samples, high cost, complex processing, etc.

Active Publication Date: 2020-01-17
中国人民解放军东部战区疾病预防控制中心
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Problems solved by technology

However, these methods require high cost, specific equipment, time-consuming and complicated sample processing, which limits their wide application in daily life.

Method used

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  • Preparation and application of echovirus type 9 vp1 protein specific antigenic epitope and its fusion protein
  • Preparation and application of echovirus type 9 vp1 protein specific antigenic epitope and its fusion protein
  • Preparation and application of echovirus type 9 vp1 protein specific antigenic epitope and its fusion protein

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Embodiment Construction

[0074] Detailed description of the embodiment of the present invention:

[0075] Analysis, Gene Synthesis and Expression of Echovirus Type 9 Surface Protein VP1 Epitope

[0076] Through the computer analysis of the amino acid sequence of the surface protein VP1 of echovirus type 9, the VP1 protein fragments of echovirus type 9 containing strong epitopes were screened out, and four protein fragments containing strong epitopes were found, respectively 75 Amino acid to amino acid 102, amino acid 127 to amino acid 140, amino acid 204 to amino acid 237, amino acid 257 to amino acid 300. The four protein fragments are linked by two glycines and one serine to form an antigenic epitope fusion protein with a total length of 128 amino acids. Codons preferred by both eukaryotes and prokaryotes are selected, and a new gene sequence of the above-mentioned epitope fusion protein is chemically synthesized. Added at the 5' end of the synthetic gene fragment Bam Restriction site for HI, wit...

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Abstract

The invention provides echovirus type-9 VP1 protein specific antigen epitope and a preparation method and application of a fusion protein thereof, and relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Computer analysis is carried out on the amino acid sequence of the echovirus type-9 surface protein VP1 to screen out protein fragments containing strong specific antigen epitope, namely 75th amino acid-102nd amino acid, 127th amino acid-140th amino acid, 204th amino acid-237th amino acid and 257th amino acid-300th amino acid, and the four protein fragments are connected through two glycines and one serine to form one antigen epitope fusion protein. Codons preferred by both eukaryon and prokaryote are selected for chemical synthesis of a brand new gene sequence of the antigen epitope fusion protein, the genetic engineering technology is utilized to prepare the antigen epitope fusion protein of the echovirus type-9 VP1 protein by expression, and the antigen epitope fusion protein is used for developing an echovirus antibody detection kit and preparing echovirus monoclonal antibody and polyclonal antibody.

Description

technical field [0001] The preparation and application of specific antigenic epitope of echovirus type 9 VP1 protein and its fusion protein in the present invention relate to the screening of surface VP1 protein fragments of echovirus type 9 containing strong antigenic epitopes, and using genetic engineering technology to prepare Fusion protein of echovirus type 9 VP1 protein epitope. Select the codons favored by both eukaryotic and prokaryotic organisms, chemically synthesize the gene sequence of a new VP1 protein antigen epitope fusion protein, and express the fusion protein by genetic engineering technology. The expressed fusion protein can be used for Echo virus vaccines and antibody detection reagents Development, etc., the present invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique [0002] Enteric Cytopathogenic Human Orphan Virus (ECHO), referred to as Echovirus, is a type of single-stranded posit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70C07K16/10C07K16/06G01N33/68G01N33/569
CPCC07K14/005C07K16/06C07K16/1009C07K2319/00C12N15/62C12N15/70C12N2770/32022G01N33/56983G01N33/68G01N2469/20
Inventor 李越希李素梅李佳萌潘英齐勇王新国李素芹陈晨陈红霞徐亦非
Owner 中国人民解放军东部战区疾病预防控制中心
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