Preparation and application of echovirus type 1 VP1 protein specific antigen epitope and fusion protein thereof

A fusion protein and antigenic epitope technology, applied in the fields of genetic engineering technology, vaccines and diagnostic reagents, can solve the problems of complex processing, limited wide application and high cost

Inactive Publication Date: 2016-09-21
李越希
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  • Abstract
  • Description
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Problems solved by technology

However, these methods require high cost, specific equipment, time-consuming and complicated sample processing, which limits their wide application in daily life.

Method used

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  • Preparation and application of echovirus type 1 VP1 protein specific antigen epitope and fusion protein thereof
  • Preparation and application of echovirus type 1 VP1 protein specific antigen epitope and fusion protein thereof
  • Preparation and application of echovirus type 1 VP1 protein specific antigen epitope and fusion protein thereof

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Embodiment Construction

[0066]Detailed description of the embodiment of the present invention:

[0067] Analysis, Gene Synthesis and Expression of Echovirus Type 1 Surface Protein VP1 Epitope

[0068] Through the computer analysis of the amino acid sequence of the surface protein VP1 of echovirus type 1, the VP1 protein fragments of echovirus type 1 containing strong epitopes were screened out, and two protein fragments containing strong epitopes were found, respectively. From the 75th amino acid to the 140th amino acid, from the 203rd amino acid to the 236th amino acid, the two protein fragments are connected by two glycines and one serine to form an antigenic epitope fusion protein with a total length of 103 amino acids. Codons preferred by both eukaryotes and prokaryotes are selected, and a new gene sequence of the above-mentioned epitope fusion protein is chemically synthesized. Added at the 5' end of the synthetic gene fragment Bam Restriction site for HI, with stop codons TAA and xho I rest...

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Abstract

The invention discloses preparation and application of an echovirus type 1 VP1 protein specific antigen epitope and a fusion protein thereof, and relates to the fields of gene engineering technology, vaccines and diagnostic reagents. An amino acid sequence of the echovirus type 1 surface protein VP1 is analyzed by a computer, and protein fragments, namely a protein fragment from a 75th amino acid to a 140th amino acid and a protein fragment from a 203rd amino acid to a 236th amino acid, containing strong specific antigen epitopes are screened; the two protein fragments are connected by two glycines and one serine, and the antigen epitope fusion protein is formed. Codons preferred by eukaryotes and prokaryotes are selected, and the bran-new gene sequence of the antigen epitope fusion protein is chemically synthesized; with use of the genetic engineering technology, the echovirus type 1 VP1 protein antigen epitope fusion protein is expressed and prepared, is used for development of echovirus antibody detection reagents, and is used for preparation of echovirus monoclonal and polyclonal antibodies.

Description

technical field [0001] The preparation and application of echovirus type 1 VP1 protein-specific antigenic epitopes and their fusion proteins in the present invention involve screening the surface VP1 protein fragments of echovirus type 1 proteins containing strong antigenic epitopes, and using genetic engineering technology to prepare Fusion protein of echovirus type 1 VP1 protein epitope. Select the codons favored by both eukaryotic and prokaryotic organisms, chemically synthesize the gene sequence of a new VP1 protein antigen epitope fusion protein, and express the fusion protein by genetic engineering technology. The expressed fusion protein can be used for Echo virus vaccines and antibody detection reagents Development, etc., the present invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique [0002] Enteric Cytopathogenic Human Orphan Virus (ECHO), referred to as Echovirus, is a type of single-stranded ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K16/10C12N15/62C12N15/70G01N33/68G01N33/569
CPCC07K14/005C07K16/1009C07K2319/00C12N15/62C12N2770/32322C12N2770/32331G01N33/56983G01N33/68
Inventor 李越希李素梅齐勇李佳萌潘英陈晨王新国李素芹陈红霞徐亦非
Owner 李越希
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