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Preparation and application of echovirus type 6 vp1 protein-specific antigenic epitope and its fusion protein

A fusion protein and antigenic epitope technology, applied in the fields of genetic engineering technology, vaccines and diagnostic reagents, can solve the problems of high cost, limited wide application, time-consuming samples, etc.

Active Publication Date: 2019-06-28
中国人民解放军东部战区疾病预防控制中心
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  • Abstract
  • Description
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Problems solved by technology

However, these methods require high cost, specific equipment, time-consuming and complicated sample processing, which limits their wide application in daily life.

Method used

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  • Preparation and application of echovirus type 6 vp1 protein-specific antigenic epitope and its fusion protein
  • Preparation and application of echovirus type 6 vp1 protein-specific antigenic epitope and its fusion protein
  • Preparation and application of echovirus type 6 vp1 protein-specific antigenic epitope and its fusion protein

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Embodiment Construction

[0129]Detailed description of the embodiment of the present invention:

[0130] Analysis, Gene Synthesis and Expression of Echovirus Type 6 Surface Protein VP1 Epitope

[0131] Through the computer analysis of the amino acid sequence of the surface protein VP1 of echovirus type 6, the VP1 protein fragments of echovirus type 6 containing strong epitopes were screened out, and four protein fragments containing strong epitopes were found, respectively. Amino acid 75 to amino acid 101, amino acid 127 to amino acid 141, amino acid 204 to amino acid 237, amino acid 257 to amino acid 289. The four protein fragments are linked by two glycines and one serine to form an epitope fusion protein with a total length of 117 amino acids. Codons preferred by both eukaryotes and prokaryotes are selected, and a new gene sequence of the above-mentioned epitope fusion protein is chemically synthesized. Added at the 5' end of the synthetic gene fragment Bam Restriction site for HI, with stop cod...

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Abstract

The invention discloses an enteric cytopathogenic human orphan virus 6-type VP1 protein specific epitope, a fusion protein of the enteric cytopathogenic human orphan virus 6-type VP1 protein specific epitope and a preparation method and use of the fusion protein and relates to the field of a gene engineering technology, a vaccine and a diagnostic reagent. The preparation method comprises analyzing an enteric cytopathogenic human orphan virus 6-type surface protein VP1 amino acid sequence by a computer, and screening strong specific epitope-containing protein fragments comprising 75th-101th amino acids, 127th-141th amino acids, 204th-237th amino acids and 257th-289th amino acids, wherein the four protein fragments are connected by 2 glycines and 1 serine so that the epitope fusion protein is formed. A codon in favor with eucaryotes and prokaryotes is used, a brand new gene sequence of the epitope fusion protein is chemically synthesized, through a gene engineering technology, the enteric cytopathogenic human orphan virus 6-type VP1 protein epitope fusion protein is expressed and prepared and is used for development of an enteric cytopathogenic human orphan virus antibody detection reagent and preparation of an enteric cytopathogenic human orphan virus monoclonal antibody and polyclonal antibody.

Description

technical field [0001] The preparation and application of specific antigenic epitope of echovirus type 6 VP1 protein and its fusion protein in the present invention relate to the screening of surface VP1 protein fragments of echovirus type 6 containing strong antigenic epitopes, and the preparation of Fusion protein of echovirus type 6 VP1 protein epitope. Select the codons favored by both eukaryotic and prokaryotic organisms, chemically synthesize the gene sequence of a new VP1 protein antigen epitope fusion protein, and express the fusion protein by genetic engineering technology. The expressed fusion protein can be used for Echo virus vaccines and antibody detection reagents Development, etc., the present invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique [0002] Enteric Cytopathogenic Human Orphan Virus (ECHO), referred to as Echovirus, is a type of single-stranded positive-sense RNA enterovirus wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00
Inventor 李越希李素梅李佳萌齐勇潘英李素芹陈晨王新国徐亦非
Owner 中国人民解放军东部战区疾病预防控制中心
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