SSR primer group and method for identification of purity of flue-cured tobacco variety Yunyan 87 seeds

Abstract

The invention relates to an SSR primer group and method for identification of the purity of flue-cured tobacco variety Yunyan 87 seeds. The method comprises the main steps: extracting genomic DNAs of a male parent, a female parent and a Yunyan 87 material, with the genomic DNAs as a template, adopting a pair of specific SSR primers MP3045, carrying out PCR amplification, carrying out separation and displaying of the PCR amplification products by adopting polyacrylamide gel electrophoresis, comparing differences between the Yunyan 87 and male parent and female parent amplified specific banding patterns, and thus identifying the purity of the Yunyan 87 seeds. Purity identification of the Yunyan 87 seeds can be completed generally in 5 h, and the method has the advantages of being rapid, accurate, low in cost, and simple in operation, can relatively replace a traditional method mainly identifying the purity of tobacco seeds through phenotypes, and has relatively good application prospect and production value in tobacco production.

Description

technical field [0001] The invention relates to a method for identifying the purity of crop seeds, in particular to an SSR primer set and method for identifying the purity of flue-cured tobacco variety Yunyan 87 seeds, and belongs to the technical field of tobacco molecular markers. Background technique [0002] The authenticity and purity of seeds are important indicators for evaluating the quality of seeds. High-purity seeds are crucial to reducing planting risks in agricultural production and ensuring economic benefits. To this end, the People's Republic of China Tobacco Industry Standard Tobacco Seed Inspection Regulations (YC / T20-1994) stipulates the purity requirements of tobacco seeds. The purity of the original species is not less than 99.9%. Not less than 99.0%. [0003] The main variety of flue-cured tobacco, Yunyan 87, was bred by Yunnan Institute of Tobacco Science and China Tobacco Breeding Research (South) Center with Yunyan No. 2 as the female parent and K32...

AI Technical Summary

Problems solved by technology

The first three purity identification methods above belong to the macroscopic traditional phenotypic identification methods. The accuracy of the identification results is greatly affected by environmental factors such as people, seasons, and climate, and the identification cycle is long, time-consuming and labor-intensive. The fourth method belongs to the microscopic The internal genetic material identification is not affected by environmental factors, and has the advantages of fast, accurate and low cost

At present, the molecular marker method for the purity identification of the main flue-cured tobacco variety Yunyan 87 has not yet been published or patented.

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