Shake-flask fermentation reaction device and using method thereof

A shake flask fermentation and reaction device technology, which is applied in the field of fermentation, can solve the problems that cannot be demonstrated, does not consider the difference in stirring mode, and cannot obviously improve the shake flask fermentation level, etc., and achieves exquisite structure, improved shake flask fermentation level, and improved Effects of Irregular Mobility

Active Publication Date: 2016-09-21
YILI CHUANNING BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned shake flask fermentation optimization work is similar to the patented "baffle shake flask", the authorized announcement number is CN204058463U, which was copied from large-scale fermentation equipment, and did not consider the difference in the stirring mode of the two fermentations, and could not be passed through fluid power. It has been proved by relevant knowledge, and it has been verified many times that baffle shake flasks cannot significantly improve the fermentation level of existing traditional shake flasks.

Method used

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  • Shake-flask fermentation reaction device and using method thereof
  • Shake-flask fermentation reaction device and using method thereof
  • Shake-flask fermentation reaction device and using method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] like Figure 1-3 As shown, the built-in includes a first longitudinal board 1 and a second longitudinal board 2. The first longitudinal board 1 and the second longitudinal board 2 are connected to each other and are perpendicular to each other. The central axis of the first longitudinal board 1 and the second longitudinal board 2 The central axes overlap.

[0050] Preferably, both the first longitudinal plate 1 and the second longitudinal plate 2 are circular plates of equal size.

[0051] Preferably, the first longitudinal plate 1 and the second longitudinal plate 2 are made of stainless steel or silica gel.

Embodiment 2

[0053] like Figure 4-6 As shown, a transverse plate 3 is added in Embodiment 1, and the transverse plate 3 and the first longitudinal plate 1 and the second longitudinal plate 2 are all cross-connected and perpendicular to each other, the circumference of the transverse plate 3, the circumference of the first longitudinal plate 1, The circumferences of the second longitudinal plates 2 are all on the same spherical surface, so that the joints between the rigid part plate and the plate on the surface of the sphere are changed from the original two to six.

Embodiment 3

[0055] like Figure 7-9 As shown, in the second embodiment, two second longitudinal plates a4 are added, the second longitudinal plates a4 and the first longitudinal plates 1 are cross-connected, the second longitudinal plates a4 are parallel to the second longitudinal plates 2, and the second longitudinal plates a4 The circumferences of the spheres are all on the spherical surface, so that the number of rigid parts on the surface of the sphere changes from six to fourteen.

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Abstract

The invention discloses a shake-flask fermentation reaction device and a using method thereof, and belongs to the field of fermentation. The fermentation reaction device comprises a reactor and an implant, wherein the implant is arranged in the reactor; and a gap is kept between the implant and the reactor. The fermentation reaction device disclosed by the invention, by improving the internal structure of the bio-reactor and on the basis of the knowledge of fluid dynamics, can enhance solid-liquid mixing and gas-liquid mixing effects in the reactor, so that a shake-flask fermentation level under an original process is optimized; and furthermore, a shake-flask fermentation process of some variety can be further improved, so that the limit of the shake-flask fermentation level of the original process is broken through.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a reaction device capable of improving the fermentation level of shake flasks. Background technique [0002] Fermentation refers to the process in which people use the life activities of microorganisms under aerobic or anaerobic conditions to prepare the microbial cells themselves, or direct metabolites or secondary metabolites. Fermentation is sometimes also written as ferment, and its definition varies depending on the occasion of use. The so-called fermentation usually refers to a certain decomposition process of organisms for organic matter. Fermentation is a biochemical reaction that human beings came into contact with earlier, and it is widely used in food industry, biology and chemical industry. [0003] Shake flask fermentation is the selection, expansion, and ability testing of the strains required for the above-mentioned fermentation process. In addition to providing strai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/24C12M1/02
CPCC12M23/08C12M23/34C12M27/02
Inventor 程玲利赵鑫张婷袁彬青周路冯雪贵姜开亮赵晓龙余洪邓旭衡刘思川葛均友万阳浴
Owner YILI CHUANNING BIOTECH CO
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