A kind of preparation method of protein iron supplement iron
An iron supplement and protein iron technology, applied in the field of blood supplements, can solve the problems that users are difficult to take for a long time, not easy for consumers to accept, interfere with drug metabolism, etc., so as to reduce side effects and adverse reactions, and reduce side effects and adverse reactions. , the effect of reducing costs
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Embodiment 1
[0021] (1) Disperse the commercially available magnetic iron oxide nanoparticles (average particle size less than 100nm) based on ferroferric oxide (average particle size less than 100nm) in a phosphate buffer solution with a pH of 8 at a ratio of 0.3g / mL, and then press the buffer solution Add 2.5% glutaraldehyde to the volume, and stir for 3 hours in the dark to cross-link the glutaraldehyde and magnetic iron oxide nanoparticles, wash off the uncross-linked glutaraldehyde molecules with distilled water, and disperse the nanoparticles by ultrasonic treatment for 10 s, then Ferric ions (FeCl 3 , 20mmol / L), stirred and reacted for 5h, washed away excess iron ions with distilled water, and prepared as a magnetic nanoparticle carrier;
[0022] (2) Dilute the egg white 8 times, adjust the pH to 8.5, then add the magnetic nanoparticle carrier by 0.5% of the solution weight, stir for 10 minutes, and make the ovotransferrin in the solution and the ferric iron on the magnetic nanopart...
Embodiment 2
[0026] (1) is identical with the step (1) of embodiment 1;
[0027] (2) Milk is diluted 5 times, and the pH of the solution is adjusted to 6.8, then the magnetic nanoparticle carrier is added by 0.02% of the solution weight, and stirred for 5 minutes, so that the transferrin in the solution and the ferric ion on the magnetic nanoparticle carrier combined;
[0028] (3) Adsorb the magnetic nanoparticle carrier that has absorbed transferrin to the bottom of the solution with a magnet, discard the upper layer solution, wash the lower floor solid with a buffer solution with a pH of 6.8, then transfer to a buffer solution with a pH of 3.2, and stir for 60 Minutes, the combined transferrin is released from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier is separated from the solution with a magnet again, and FeCl is added to the solution 3 (20mmol / L) reaction, obtain iron binding transferrin after drying;
[0029] (4) Take 100g of iron-bound transferrin, 0.1g o...
Embodiment 3
[0031] (1) is identical with the step (1) of embodiment 1;
[0032] (2) Dilute the egg white 10 times, adjust the pH of the solution to 9.5, then add the magnetic nanoparticle carrier by 1% of the solution weight, stir for 60 minutes, and make the transferrin in the solution and the ferric iron on the magnetic nanoparticle carrier ion binding;
[0033] (3) Adsorb the magnetic nanoparticle carrier that has absorbed transferrin to the bottom of the solution with a magnet, discard the upper layer solution, wash the lower floor solid with a buffer solution with a pH of 9.5, then transfer to a buffer solution with a pH of 4.8, and stir for 5 Minutes, the combined transferrin is released from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier is separated from the solution with a magnet again, and FeCl is added to the solution 3 (5mmol / L) reaction, obtain iron binding transferrin after drying;
[0034] (4) Take 100g of iron-binding transferrin, 0.5g of ferrous fu...
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