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Chimeric virus-like particles carrying muc1 tumor antigen epitope pdtrp and its application in pancreatic cancer

A tumor antigen, pancreatic cancer technology, applied in the fields of medicine and bioengineering, can solve the problems of vaccine prevention or treatment of pancreatic cancer that have not been reported in the literature, and achieve the effects of low production cost, easy promotion and simple method

Active Publication Date: 2020-01-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the preparation of chimeric virus-like particle vaccines carrying the MUC1 tumor antigen epitope PDTRP by bioengineering methods, and there is no literature report on the use of this vaccine in the prevention or treatment of pancreatic cancer

Method used

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  • Chimeric virus-like particles carrying muc1 tumor antigen epitope pdtrp and its application in pancreatic cancer
  • Chimeric virus-like particles carrying muc1 tumor antigen epitope pdtrp and its application in pancreatic cancer
  • Chimeric virus-like particles carrying muc1 tumor antigen epitope pdtrp and its application in pancreatic cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Experimental Materials:

[0031] Table 1

[0032]

[0033]

[0034] 1. Chimeric HBc-VLP gene synthesis

[0035] a, related information of gene synthesis: gene name: HBcP gene length: 459bp, cloning vector: pET28a, cloning site: NdeI / XhoI, cloning vector resistance: Kan, structure diagram ( figure 1 ).

[0036] b. The synthesis of DNA fragments by the phosphoramidite triester method has the characteristics of high efficiency, fast coupling and relatively stable starting reactants. This method completes the synthesis of DNA strands on a solid-phase carrier, and the synthesis direction is along the 3' end to the 5' end of the primer to be synthesized, and the adjacent nucleotides are connected by 3'→5' phosphodiester bonds .

[0037] c. Vector construction: Digest the synthetic target gene fragment and the vector with restriction endonucleases XhoI and NdeI respectively

[0038] Enzyme cutting system:

[0039]

[0040]

[0041] d. Connection:

[0042] Co...

Embodiment 2

[0120] PDTRP polypeptide can protect BALB / c mice after pancreatic cancer cell tumor formation

[0121] Pancreatic cancer chimeric HBc-VLP vaccine with MUC1 tumor antigen epitope PDTRP gene and HBc-VLP control without MUC1 tumor antigen epitope PDTRP were immunized in vivo at week 0 and week 4, respectively, and at week 2 after the last immunization. Weeks later, PANC1-PDTRP tumor cells were inoculated subcutaneously, and the tumor formation in mice of different experimental groups was observed.

[0122] Experimental results

[0123] See Figure 7 , it can be seen from the experimental results that compared with the negative control, the injection of the chimeric vaccine carrying MUC1 can inhibit the tumorigenesis of tumor cells.

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Abstract

The invention relates to the field of bioengineering, in particular to an MUC1 tumor antigen epitope PDTRP carrying chimeric virus-like particle and application thereof to pancreatic cancer and provides a preparation method of the MUC1 tumor antigen epitope PDTRP carrying chimeric virus-like particle. Induction of lasting body fluid and cell response is successfully realized. The method is simple, low in production cost and easy to popularize.

Description

technical field [0001] The invention relates to the technical fields of medicine and bioengineering, in particular to a chimeric virus-like particle carrying MUCl tumor antigen epitope PDTRP and its application in pancreatic cancer. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive system with a poor prognosis. Actively looking for effective methods for the treatment of pancreatic cancer has become an international hot issue. MUC1 is a member of the Mucin family of mucins, present on the surface of normal ductal epithelial cells and their derived tumor cells, and consists of a polypeptide core and side branch sugar chains. The extracellular segment of its core peptide contains many tandem repeats (variable numbers of tandem repeat, VNTRs) of 20 amino acids (SAPDTRPAPGSTAPPAHGVT). Sugar chains account for more than 50% of the weight of the MUC1 protein, and most of them are connected to the peptide backbone by O-type glycosidic bon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/62C12N15/70C12N1/21C12N7/04A61K39/00A61K39/385A61P35/00C12R1/19
CPCA61K39/0011A61K2039/5258A61K2039/6075C07K14/005C07K14/4727C07K2319/00C12N2730/10122C12N2730/10123C12N2730/10142
Inventor 孙畅李兆申
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY