Method for simultaneous determination of levo-glucan, mannan and galactan in aerosol by high performance liquid chromatography-tandem quadrupole mass spectrometry

Abstract

A method for synchronously measuring levoglucosan, mannan and galactosan in aerosol for high performance liquid chromatography-tandem quadrupole mass spectrometry combination includes the steps that levoglucosan, mannan and galactosan in an aerosol sample are subjected to ultrasonic extraction with ultrapure water and detected by means of a high performance liquid chromatography-tandem quadrupolemass spectrometer; ammonia water is adopted as a mobile phase; an electrospray ionization mode suitable for polar molecule analysis is adopted as an ion source of a mass spectrometry, ion source parameters and compound parameters optimized many times are adopted in a mass spectrometric method, and qualitative and quantitative analysis is conducted in an anion and multiple-reaction monitoring mode.By means of the external standard curve method, the linear relation of L, M and G is good in the range of 0.01<-1> microgramme per milliliter, the correlation coefficient is larger than 0.99, the detection limit of L is smaller than 0.005 microgramme per milliliter, the detection limits of M and G are both smaller than 0.01 microgramme per milliliter, experiment repeatability is good, relative standard deviations of repetitive experiments of L, M and G are 1.31%, 4.48% and 1.31% respectively, and the adding standard recovery rates of L, M and G all reach 97% or above.

Description

technical field

[0001] The invention relates to a method for synchronous determination of levoglucosan, mannan and galactan in aerosol by high performance liquid chromatography-tandem quadrupole mass spectrometry. Background technique

[0002] Biomass combustion, such as forest fires and straw burning, will emit toxic substances such as polycyclic aromatic hydrocarbons and halogenated organic pollutants, which will affect human health. At the same time, it will also emit greenhouse gases and atmospheric particles, which will affect the atmospheric environment and climate change. The composition is extremely complex, so it is very important to use molecular tracers to study the impact of material combustion on the atmospheric environment in regional or local areas.

[0003] Levoglucosan (1,6-anhydro-β-d-glucose, or Levoglucosan, L) scientific name 1,6-anhydro-β-D-glucose, is a product of cellulose pyrolysis above 300 ° C, There is no other source. It is widely used as a tra...

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