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Primer group and reagent box used for detecting HLA-B* (human leukocyte antigen)-1502 allelic genes

An allele and HLA-B technology, applied in the field of biomedical clinical molecular detection, can solve the problems of easy false positive results and affecting accuracy

Active Publication Date: 2016-11-16
JIANGSU WEIHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the Taqman fluorescent quantitative PCR method designs one or more pairs of HLA-B*1502-specific primers and corresponding probes for PCR amplification. Considering that HLA polymorphism is very high, the results are prone to false positives, so affect its accuracy

Method used

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  • Primer group and reagent box used for detecting HLA-B* (human leukocyte antigen)-1502 allelic genes
  • Primer group and reagent box used for detecting HLA-B* (human leukocyte antigen)-1502 allelic genes
  • Primer group and reagent box used for detecting HLA-B* (human leukocyte antigen)-1502 allelic genes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Raw materials and equipment:

[0038] 1.1 Contents of the kit:

[0039] 1) Fluorescent quantitative PCR reaction with 8 tubes, each 8 tubes can be used for 2 sample tests, each test requires 3 tubes (MIX1, MIX2 and MIX3):

[0040] The black marks on the 8-connected tubes are MIX1, MIX2, MIX3 in turn, and the same tubes are MIX1, MIX2, MIX3 after emptying one tube, as shown in Figure 3.

[0041] 2) 3 pairs of specific upstream and downstream amplification primers and corresponding probes are freeze-dried in a specific arrangement at the bottom of the 8-tube PCR reaction tube, and a pair of internal control primers and corresponding probes are added to the bottom of the 8-tube tube for each PCR reaction; all Primers and probes are lyophilized at the bottom of PCR reaction tubes.

[0042] The distribution of 3 pairs of specific amplification primers and corresponding probes in the 8-tube PCR reaction is as follows:

[0043]

[0044]

[0045] A pair of internal ...

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Abstract

The invention provides a PCR (polymerase chain reaction) amplification primer group and Taqm probes used for detecting HLA (human leukocyte antigen)-B*1502 allelic genes. The PCR amplification primer group and Taqm probes include two pairs of primers designed according to specific sequence of HLA-B*1502 and two corresponding probes, further include one pair of probes used for filtering out false positive results of the HLA-B*1502 and one corresponding probe, and further include one pair of internal-contract primers and one corresponding probe. The primer group has the technical advantages that the genes of the HLA-B*1502 are positive or not are determined through two reaction tubes, the false positive result of the HLA-B*1502 is eliminated through one reaction tube, and the false positive results of the genes of the sampled HLA-B*1502 are eliminated by adding the internal-control primers and the probes in reaction holes.

Description

technical field [0001] The invention relates to a method and a kit for detecting HLA-B*1502 alleles, belonging to the field of biomedical clinical molecular detection. Background technique [0002] Pharmacogenomics, also known as genomic pharmacology or genomic pharmacology, is Pharmacology study A branch of , defined as the Genomics on the basis of gene expression or single Nucleotide Linking polymorphisms in drugs to the efficacy or toxicity of drugs, studying how drugs act differently due to genetic variation. In short, pharmacogenomics is dedicated to studying the influence of individual gene structure on drug response, discovering associated genes and their mechanism of action and function, and applying them to clinical disease diagnosis and determination of drug dosage. The combination of gene research data and clinical practice can help to grasp the patient profile more accurately, choose the optimal treatment plan, and further help the identification of pati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 陈炤源王浩张金涛章婷婷
Owner JIANGSU WEIHE BIOTECH
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