HLA-B*1502 detection kit

A technology of HLA-B and detection kits, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., and can solve the problems of unsatisfactory HLA-B*1502 detection methods and unsuitability for large-scale promotion and use , to achieve the effects of easy promotion, short detection time, and short detection time

Inactive Publication Date: 2016-02-03
BEIJING JINQI BIOLOGICAL TECH CO LTD
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Problems solved by technology

[0008] The problem to be solved by the present invention is that the existing HLA-B*1502 detection method is not ideal and is not suitable for large-scale promotion and use

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Embodiment Construction

[0037]In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0038] An HLA-B*1502 detection primer for detecting the C allele of rs10484555, including the following primers:

[0039] 5'-AAATTGTTAAAGGTACTTGGAAAG-3' (SEQ ID No.1, hereinafter referred to as F3)

[0040] 5'-CATCTAAAGTATACAATTCAATGGTT-3' (SEQ ID No.2, hereinafter referred to as B3)

[0041] 5'-GCCATGAAGTAAATCCATAAATTTTGAGTTTGTGCCTGTCAAAGCTAGGA-3' (SEQ ID No.3, hereinafter referred to as FIP)

[0042] 5'-AGAGAGACATATTATAAAGCCGTGGATCATATTCAGAGTTTTAAATCTGCA-3' (SEQ ID No. 4, hereinafter referred to as BIP).

[0043] The above-mentioned F3, B3, FIP, and BIP are primers purified by HPLC purification.

[0044] An HLA-B*1502 detection kit comprises a composition for detecting the genotype of the rs10484555 site and further detecting HLA-B*1502. The composition includes the above primers, and also i...

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Abstract

The invention provides an HLA-B*1502 detection kit and belongs to the technical field of carbamazepine use detection. The HLA-B*1502 detection kit is characterized in that the Allele-Specific LAMP technology is used for designing specific primers aiming at the C allele of rs10484555, the relevance between the C allele of rs10484555 and HLA-B*1502 is used for judging whether a patient carries HLA-B*1502 allele or not, so that personalized carbamazepine use can be guided, and problems caused by direct testing for HLA-B*1502 are avoided. The HLA-B*1502 detection kit is low in cost, short in detecting time, high in accuracy and easy to popularize.

Description

technical field [0001] The invention relates to the technical field of carbamazepine individualized drug detection, in particular to a detection kit for HLA-B*1502. Background technique [0002] Carbamazepine is a common antipsychotic drug, which is mainly used clinically for anti-epilepsy, and can also be used for trigeminal neuralgia. In some individuals, carbamazepine can cause Stevens Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), a severe skin and mucous membrane reaction that can lead to Permanently disabled or even fatal. In Caucasians, the incidence of SJS / TEN caused by carbamazepine is very low, only 1 in 10,000 to 3 in 10,000, however, in some Asian countries, including Indians in South Asia, patients taking carbamazepine and The resulting SJS / TEN is about 10 times higher than that of Caucasians. [0003] It has been shown that the increased risk of SJS / TEN is associated with HLA-B*1502, and this association is independent of whether the individual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/106C12Q2600/156C12Q2531/119C12Q2535/125
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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