HLA-B*1502 detection kit
A technology of HLA-B and detection kits, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., and can solve the problems of unsatisfactory HLA-B*1502 detection methods and unsuitability for large-scale promotion and use , to achieve the effects of easy promotion, short detection time, and short detection time
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[0037]In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.
[0038] An HLA-B*1502 detection primer for detecting the C allele of rs10484555, including the following primers:
[0039] 5'-AAATTGTTAAAGGTACTTGGAAAG-3' (SEQ ID No.1, hereinafter referred to as F3)
[0040] 5'-CATCTAAAGTATACAATTCAATGGTT-3' (SEQ ID No.2, hereinafter referred to as B3)
[0041] 5'-GCCATGAAGTAAATCCATAAATTTTGAGTTTGTGCCTGTCAAAGCTAGGA-3' (SEQ ID No.3, hereinafter referred to as FIP)
[0042] 5'-AGAGAGACATATTATAAAGCCGTGGATCATATTCAGAGTTTTAAATCTGCA-3' (SEQ ID No. 4, hereinafter referred to as BIP).
[0043] The above-mentioned F3, B3, FIP, and BIP are primers purified by HPLC purification.
[0044] An HLA-B*1502 detection kit comprises a composition for detecting the genotype of the rs10484555 site and further detecting HLA-B*1502. The composition includes the above primers, and also i...
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