Preparation method of original boletus edulis strains

The technology of a delicious boletus and a production method is applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., and can solve the problems of long growth cycle, complicated process, and high pollution rate of delicious boletus strains, and achieves the Pure, simple production method, and the effect of reducing production costs

Inactive Publication Date: 2016-11-23
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the problems of high pollution rate, long growth cycle and complex process of existing delicious boletus strains, and provide a new method for making delicious boletus original species, so that the prepared delicious boletus The original seed of liver fungus has simple process, pure strain, less pollution, simple operation, short cycle (6-8d), and cost reduction (10-15%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1, a kind of preparation method of original species of delicious boletus, is characterized in that: comprise the following steps

[0019] (1) Substratum production: calculate by weight parts, clean 150 parts of fresh pine needles, cut 150 parts of peeled potatoes into chunks, 100 parts of delicious boletus origin soil, fresh pine needles, block potatoes and origin soil Add 1000 parts of water, boil for 30-40 minutes, filter out fresh pine needles, potato residues and insoluble soil particles with gauze to obtain the filtrate, add 6 parts of tryptone, 0.52 parts of carbonamide, and 0.45 parts of beef extract to the filtrate, Vitamin B 1 0.000028 parts, 0.65 parts of dipotassium hydrogen phosphate, 0.50 parts of ferrous sulfate, 0.55 parts of calcium chloride, 10 parts of glucose, 0.010 parts of chloramphenicol, 10 parts of granular activated carbon, 10 parts of granular mature boletus fruiting body, add water to set The volume is up to 1000 parts, and the pH value is co...

Embodiment 2

[0024] 1, a kind of preparation method of original species of delicious boletus, is characterized in that: comprise the following steps

[0025] (1) Substratum production: calculate by weight parts, clean 200 parts of fresh pine needles, cut 200 parts of peeled potatoes into chunks, 150 parts of delicious boletus origin soil, fresh pine needles, block potatoes and origin soil Add 1000 parts of water, boil for 40 minutes, filter out fresh pine needles, potato residues and insoluble soil particles with gauze to obtain the filtrate, add 10 parts of tryptone, 0.60 parts of carbonamide, 0.55 parts of beef extract, and vitamin B 1 0.000035 parts, 0.75 parts of dipotassium hydrogen phosphate, 0.60 parts of ferrous sulfate, 0.65 parts of calcium chloride, 15 parts of glucose, 0.015 parts of chloramphenicol, 15 parts of granular activated carbon, 15 parts of granular mature boletus fruiting bodies, add water to set The volume is up to 1000 parts, and the pH value is controlled to be 5....

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PUM

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Abstract

The invention discloses a preparation method of original boletus edulis strains. The original boletus edulis strains are prepared through the steps that a specific original boletus edulis strain culture medium is prepared from fresh pine needles, peeled potatoes, boletus edulis locality soil, tryptone, carbamide, a beef extract, vitamin B1, dipotassium hydrogen phosphate, ferrous sulfate, calcium chloride, glucose, chloramphenicol, granular active carbon, granular mature boletus edulis sporophores, gibberellin and the like, and then culture medium sterilizing, inoculating and strain culturing are conducted. Compared with the prior art, the preparation method of the original boletus edulis strains has the quite outstanding advantages that simpleness is achieved, and the stains are pure; the production cost can be reduced by 10%-15%; strain contamination can be reduced by 25% or above; the mycelium biomass (136.42 mg/kg) is increased by 25 mg/kg or above compared with a traditional method, and the infection intensity (75%) of the strains on trees can be improved by 20% or above compared with the traditional method.

Description

technical field [0001] The invention relates to the technical field of producing edible and medicinal fungus strains, in particular to a method for producing the original species of delicious boletus. Background technique [0002] Delicious boletus (Boletus edulis) is also known as bigfoot mushroom and white boletus. Fruiting bodies are medium to large. The cap is flat hemispherical or slightly flattened, non-sticky, smooth, with pure edges, yellowish brown, earthy brown or reddish brown. The flesh of the bacteria is white, thick, and does not change color after injury. Fungal tubes are initially white, then pale in color, straight or nearly twinned, or sunken around the stalk. The mouth of the tube is round, the base is slightly enlarged, light brown or light yellowish brown, and the inside is solid. [0003] Delicious boletus grows in mountainous areas of 600-1500m. It is a high-temperature fungus. The optimum temperature for mycelium growth is 23-28°C. Generally, it g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 黄先飞贺红早
Owner GUIZHOU NORMAL UNIVERSITY
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