Efficient leaf cutting propagation method for succulent plant

Abstract

The invention relates to an efficient leaf cutting propagation method for a succulent plant and belongs to the technical field of bioengineering. The efficient leaf cutting propagation method comprises the following main steps: picking off leaves from a parent body, and keeping in a shade place for 2-4 days; testing average volume of the leaves and the average volume, absorbing exogenous liquid, of the leaves, and then calculating concentrations and volumes of a plant hormone 6-BA and NAA which are required to be prepared, so that concentrations of 6-BA and NAA in the leaves are 2-3mg/L and 0.1-0.2mg/L respectively after injection; preparing 6-BA and NAA with appropriate concentrations, and injecting to the middle parts of the leaves; and putting the leaves on a mixed medium to be cultured. The efficient leaf cutting propagation method provided by the invention can enable the leaves of the succulent plant to keep higher plant hormone level, and propagation coefficient can be improved by 3-8 times compared with that of the conventional method; meanwhile, operation is simple, and hormone utilization rate is high, so that the efficient leaf cutting propagation method has great popularization potential.

Description

technical field [0001] The invention relates to a high-efficiency leaf cutting propagation method for succulent plants, belonging to the technical field of bioengineering. Background technique [0002] Succulents refer to higher plants with plant vegetative organs. They usually have three vegetative organs of roots, stems, and leaves and three reproductive organs of flowers, fruits, and seeds. The water storage tissues are generally in the leaves. The active leaves picked from the succulent plant are equivalent to a MS medium, which can be directly used for in vitro culture, and the plant organs can grow from the growth point, and finally grow into a complete plant. However, due to the slow speed of self-propagation, people's demand for succulents cannot be met, causing the price of succulents to increase day by day on the market. [0003] If no measures are taken, it will take about 15 days for the leaves of succulents to germinate, and it will take nearly a month for them...

AI Technical Summary

Problems solved by technology

If you use a cotton ball to apply auxin (NAA) and cytokinin (6-BA) on the growth point, the hormone will completely evaporate in about half an hour, and the leaves cannot fully absorb it, so if you apply it to the growth point every day, Then the workload is greatly increased, and the time of leaf germination is only slightly advanced, without significant effect