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Method for extracting bioactive components from Rhododendron mucronatum

A biologically active ingredient, a technology of Rhododendron azalea, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve problems such as no patents, and save reagents and production costs. Low, high extraction efficiency

Inactive Publication Date: 2017-01-04
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, no supercritical CO 2 There are no reports on extracting the active ingredients of Rhododendron albicans during the flowering period, and there are no related patents

Method used

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  • Method for extracting bioactive components from Rhododendron mucronatum
  • Method for extracting bioactive components from Rhododendron mucronatum
  • Method for extracting bioactive components from Rhododendron mucronatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The method for extracting the biologically active components in Rhododendron albicans of the present invention is as follows:

[0048] (1) Dry the white-flowered rhododendrons picked during the flowering period outdoors under natural conditions, crush them and pass them through a 200-mesh sieve;

[0049] (2) Weigh 3 g Rhododendron alba powder into the extraction kettle, and use CO 2 The fluid is the extractant, the extraction pressure is 15MPa, the extraction temperature is 35°C, and the extraction time is 2h, to obtain 0.01034g of light yellow active ingredient extract.

Embodiment 2

[0051] The method for extracting the biologically active components in Rhododendron albicans of the present invention is as follows:

[0052] (1) Dry the white-flowered rhododendrons picked during the flowering period outdoors under natural conditions, crush them and pass them through a 200-mesh sieve;

[0053] (2) Weigh 3.5g Rhododendron alba powder into the extraction kettle, and use CO 2 The fluid is the extractant, the extraction pressure is 20MPa, the extraction temperature is 45°C, and the extraction time is 1.5h, to obtain 0.01229g of light yellow active ingredient extract.

Embodiment 3

[0055] The method for extracting the biologically active components in Rhododendron albicans of the present invention is as follows:

[0056] (1) Dry the white-flowered rhododendrons picked during the flowering period outdoors under natural conditions, crush them and pass them through a 200-mesh sieve;

[0057] (2) Weigh 3.7g Rhododendron alba powder into the extraction kettle, with CO 2 The fluid is the extractant, the extraction pressure is 25MPa, the extraction temperature is 40°C, and the extraction time is 2.5h, to obtain 0.02934g of light yellow active ingredient extract.

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Abstract

The invention provides a method for extracting bioactive components from Rhododendron mucronatum. The method includes: grinding Rhododendron mucronatum picked at a flowering stage, adding into an extraction kettle, and taking CO2 fluid as an extracting agent to extract light yellow active component extract under the extraction pressure of 15-25 MPa and the extraction temperature of 35-45 DEG C for 1.5-2.5 h. In a provided technical parameter range, the method is high in extraction efficiency and extraction speed, simple in process, reagent saving, low in energy consumption, low in cost and low in pollution during extraction of the bioactive components from the Rhododendron mucronatum picked at the flowering stage, thereby being superior to other extraction methods adopting traditional extraction techniques.

Description

technical field [0001] The invention belongs to the technical field of separation of natural products, and in particular relates to a method for extracting active components in Rhododendron albicans. Background technique [0002] The main phytochemical components of Rhododendron are Nepetaolein, Nepetaolein, Quercetin, Kaempferin, Hyperin, Red Pigment, Sitosterol, etc. The active ingredients of Rhododendronaceae plants have good antibacterial and anti-inflammatory effects. For example, the antibacterial effects of Rhododendron xiuli, Rhododendron Wenchuan, and dwarf loquat are stronger than or equivalent to that of Huanglian. But in recent years, it has received more attention as a neuropharmacological substrate. [0003] James A.Klock et al. extracted chemical components from Rhododendron japonica as cardiovascular drug treatment; Yoshiki et al. extracted two new chromane derivatives 1 and chromane derivative 2 from Rhododendron xing'an, another compound Chromene 3 has po...

Claims

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Application Information

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IPC IPC(8): A61K36/45
CPCA61K36/45A61K2236/37
Inventor 郝宝成班旦拉巴次旦刘宇梁剑平陶蕾王学红尚若锋郭文柱杨珍
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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