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Nano Liu-Shen-Wan solution and preparation method thereof

A kind of Liushen pill and nano technology, applied in the direction of pharmaceutical formula, emulsion delivery, medical preparations with inactive ingredients, etc., can solve the problems of high treatment cost, narrow indications, high risk, improve the quality of life of patients, reduce pain, Easy-to-use effects

Active Publication Date: 2017-01-25
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems of narrow indications, high risk, and high treatment cost in the treatment of primary liver cancer by means of existing technologies in the background, the present invention provides a nano-Liushen pill solution and its preparation that can have a better preventive effect on liver cancer induced by chemical poisons. method

Method used

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  • Nano Liu-Shen-Wan solution and preparation method thereof
  • Nano Liu-Shen-Wan solution and preparation method thereof
  • Nano Liu-Shen-Wan solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Nano Liushen Wan solution prevents and treats primary liver cancer in mice

[0043] Animals: 128 SPF-grade Kunming KM male mice (provided by Hubei Experimental Animal Research Center), weighing 18-22g, were given free access to water and diet, and the room temperature was kept at 25±1°C, with a light-dark cycle of 12 hours.

[0044] Preparation of Nano Liushen Pill Solution: Dissolving the freeze-dried powder of Liushen Pill alcohol extract and egg yolk lecithin in sesame oil to prepare the oil phase, the final concentration of egg yolk lecithin is 6% W / V. The aqueous phase was prepared by dissolving Tween 80 and glycerol in ultrapure water, the final concentration of Tween 80 was 1.25% V / V, and the final concentration of glycerin was 2.81% V / V. Under the condition of a constant temperature water bath at 60°C, drop the oil phase into the water phase, and stir for 15 min under the condition of 1200r / min, the volume ratio of the oil phase to the water phase is 1...

Embodiment 2

[0052] Example 2 Nanometer Liushen Pill relieves pain

[0053] Animals: 128 SPF-grade Kunming KM male mice (provided by Hubei Experimental Animal Research Center), weighing 18-22g, were given free access to water and diet, and the room temperature was kept at 25±1°C, with a light-dark cycle of 12 hours.

[0054] Preparation of Nano Liushen Pill Solution: Dissolving the freeze-dried powder of Liushen Pill alcohol extract and egg yolk lecithin in sesame oil to prepare the oil phase, the final concentration of egg yolk lecithin is 6% W / V. The aqueous phase was prepared by dissolving Tween 80 and glycerol in ultrapure water, the final concentration of Tween 80 was 1.25% V / V, and the final concentration of glycerol was 2.81% V / V. Under the condition of a constant temperature water bath at 60°C, drop the oil phase into the water phase, and stir for 15 min under the condition of 1200r / min, the volume ratio of the oil phase to the water phase is 1:4. Under the condition of 10000rpm u...

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Abstract

The invention discloses a nano Liu-Shen-Wan solution and a preparation method thereof. The nano Liu-Shen-Wan solution is a nano Liu-Shen-Wan alcohol extract oil-in-water preparation and comprises a high-dose nano Liu-Shen-Wan alcohol extract oil-in-water preparation or a low-dose nano Liu-Shen-Wan alcohol extract oil-in-water preparation. The nano Liu-Shen-Wan solution has an excellent effect of preventing and treating liver cancer induced by chemical toxicant; obvious tumor nodules can be observed by naked eyes in liver tissue of four mice accounting for 50% of the nano DEN group of mice; tumor nodules can be observed in liver tissue of three mice accounting for 37.5% of the high-dose nano Liu-Shen-Wan group; the obvious tumor nodules cannot be observed in the mice of the low-dose nano Liu-Shen-Wan group; a HE dyeing result shows that the liver tissue of the mice in the nano DEN group has moderate disorder; and the disorder degree of the liver tissue of the mice treated by the high-dose nano Liu-Shen-Wan solution and the low-dose nano Liu-Shen-Wan solution is low.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a nanometer Liushen pill solution and a preparation method. Background technique [0002] Primary liver cancer has a high mortality rate and ranks third in the death order of malignant tumors after gastric cancer and esophageal cancer, and the disease develops rapidly, seriously endangering human life and health (Hepatocellular carcinoma.Recenti ProgMed, 2016, 107 (7): 386-394). Most liver cancer patients are not only affected by malignant diseases but also suffer from chronic liver diseases, with poor prognosis and poor quality of life (Nutrition and Hepatocellular Cancer. Gastrointest Tumors, 2016, 2(4): 188-94). The incidence of liver cancer in my country ranks first in the world, and the number of deaths is about 383,000 per year, accounting for 51% of the total number of deaths from liver cancer worldwide (The global burden of liver disease: the major impact of China. Hepatology,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K47/44A61K47/26A61K35/65A61P35/00A61K35/618A61K35/413A61K35/55A61K33/36A61K31/045
CPCA61K9/0053A61K9/107A61K31/045A61K33/36A61K35/413A61K35/55A61K35/618A61K35/65A61K47/26A61K47/44A61K2300/00
Inventor 李玉桑陈夕桢唐和斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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