A kind of NK cell culture medium

A technology of NK cells and culture medium, applied in the direction of cell culture active agent, culture process, animal cells, etc., can solve the problems of NK cells, such as risks, and achieve the effect of good safety, excellent effect, and good killing activity.

Active Publication Date: 2020-02-21
南通市宝通康生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method significantly increases the number of cultured NK cells, since K562 cells belong to tumor cells, there are risks in the use of cultured NK cells

Method used

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  • A kind of NK cell culture medium
  • A kind of NK cell culture medium
  • A kind of NK cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The present embodiment NK cell culture medium comprises each component of following ratio:

[0028]

[0029]

[0030] The preparation method of the above-mentioned medium is as follows: Take the preparation of 1L as an example, measure or weigh 60mL plasma, 50μg interleukin-2, 80μg anti-CD3 monoclonal antibody, 100mg IGF-1, 100μg polypeptide and 0.8g Cordyceps polysaccharide In 810mL of serum-free basal medium, mix at 15-25°C for 3-5min. After it is completely dissolved, dilute to 1L with double-distilled water, mix well, and set aside.

Embodiment 2

[0032] The present embodiment NK cell culture medium comprises each component of following ratio:

[0033]

[0034] The preparation method of above-mentioned culture medium is the same as embodiment 1.

Embodiment 3

[0036] The present embodiment NK cell culture medium comprises each component of following ratio:

[0037]

[0038] The preparation method of above-mentioned culture medium is the same as embodiment 1.

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Abstract

The invention provides a NK cell medium. The NK cell medium comprises the following components in proportion: 80-90% of a serum-free basic medium, 4-6% of plasma, 10-100 mu g / L of interleukin-2, 50-150 mu g / L of anti-CD3 monoclonal antibody, 50-200 mg / L of para-insulin first growth factor, 50-300 mu g / L of polypeptide, 0.6-1 g / L of cordyceps polysaccharide and the balance of water. The NK cell medium is safe, and NK cells cultured by the NK cell medium are proliferated rapidly and stably, and have high killing activity to tumor cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an NK cell culture medium. Background technique [0002] NK cells play an important role in anti-virus and anti-tumor, and because they do not require primary immune activation, they have unique application prospects in adoptive immunotherapy. The application of NK cells in immunotherapy is limited due to the inability to obtain large quantities and high purity of human NK cells. The method of in vitro expansion to obtain sufficient quantity and high purity of human NK cells is an important basic platform for studying the function of NK cells, especially for adoptive immunotherapy in recent years. [0003] So far, the culture conditions of NK cells obtained in vitro are usually co-stimulation with soluble IL-2, IL-12, IL-15 and other cytokines, but only dozens of times the expansion of NK cells can be achieved. It is difficult to obtain a large amount of expansion of NK ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0646C12N2500/90C12N2501/2302C12N2501/515C12N2501/90C12N2501/998
Inventor 沈珎宇王亚力
Owner 南通市宝通康生物工程股份有限公司
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