High-density fermentation and culture method of Haemophilus parasuis

A technology for the fermentation and cultivation of Haemophilus suis, which is applied in the field of high-density cultivation of Haemophilus parasuis, can solve the problems of economic loss, low culture density, and long culture time in the pig industry, and achieve good safety and immune efficacy

Inactive Publication Date: 2017-02-22
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, it has been confirmed that Haemophilus parasuis exists and is prevalent in the vast majority of pig farms in my country, mainly affecting piglets aged 5 to 8 weeks, the incidence rate is generally 10% to 15%, and the mortality rate can reach more than 50% in severe cases. Huge economic loss to my country's pig industry
However, there are many problems in the current culture techniques and methods for Haemophilus parasuis, such as long culture time and low culture density, all of which lead to high culture costs.

Method used

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  • High-density fermentation and culture method of Haemophilus parasuis
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  • High-density fermentation and culture method of Haemophilus parasuis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 prepares culture medium of the present invention according to different formulations

[0013] 1 Culture medium preparation Prepare 6 kinds of medium with different components according to different medium formulations. The specific formula is shown in Table 1.

[0014] Table 1 Liquid medium preparation method

[0015]

Embodiment 2

[0016] The screening of embodiment 2 different formula liquid culture medium:

[0017] The Haemophilus parasuis YBH05 strain (Haemophilus parasuis has been sent to No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing on November 23, 2011) Central preservation, the preservation number is: CGMCC No.5480) bacterial solution, inoculate the above-mentioned 6 kinds of liquid culture mediums respectively according to 5%, put 37 ℃, 150r / min vibrate culture, culture time is 24 hours, respectively at different culture time Samples were taken for viable counts. The screening test results of liquid culture medium with different formulas showed that the liquid culture medium prepared according to formula 3 and formula 4 had a better culture effect, and the number of viable bacteria in 14 to 16 hours was not less than 10 9 CCU / ml, formula 5 and formula 6, due to the high amount of supplementary liquid components such as serum in the medium, the medium is too rich in nutrients, resu...

Embodiment 3

[0021] Embodiment 3: culture medium comparison of the present invention and tryptone soybean broth liquid medium

[0022] After recovering and purifying the freeze-dried seeds of the YBH05 strain, inoculate the liquid culture medium of tryptone soybean broth and the liquid medium of formula 3 of the present invention at 3-5%, respectively, place them at 37° C., and vibrate at 150 r / min for 24 hours. Samples were taken at different times of culture for counting viable bacteria. The test results show that, using the culture medium of the present invention, the amount of viable bacteria in different culture times is higher than that of the tryptone soybean broth liquid culture medium. See Table 3 for the results of viable bacteria at different incubation times.

[0023] Table 3: Results of the selection test of the medium

[0024]

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Abstract

The invention provides a high-density fermentation and culture method of Haemophilus parasuis. The stirring rate, dissolved oxygen, pH value and culture time in the culture process are controlled by using a high-density fermentation tank culture technique, thereby being beneficial to the growth of Haemophilus parasuis; and thus, the viable count is not lower than 10<9> CFU / ml. When the Haemophilus parasuis solution cultured by the technique is used for preparing an inactivated vaccine, the inactivated vaccine has favorable safety and immune efficacy.

Description

technical field [0001] The invention belongs to the technical field of pathogen culture, and in particular relates to a method for high-density culture of Haemophilus parasuis. Background technique [0002] Haemophilus parasuis disease, also known as porcine Grass disease ( disease), is an infectious disease characterized by multiple serositis, arthritis and meningitis caused by Haemophilus parasuis, and is one of the important bacterial infectious diseases that seriously endanger the development of the world's pig industry in recent years. At present, it has been confirmed that Haemophilus parasuis exists and is prevalent in most pig farms in my country, mainly affecting piglets aged 5 to 8 weeks, the incidence rate is generally 10% to 15%, and the mortality rate can reach more than 50% in severe cases. Cause huge economic loss to my country's pig industry. However, there are many problems in the current cultivation techniques and methods for Haemophilus parasuis, such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/21
CPCC12N1/20
Inventor 郭莉莉邹桂荣马爽邹敏胡潇
Owner YEBIO BIOENG OF QINGDAO
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