Breast cancer metastasis assessment kit

A breast cancer and kit technology, applied in the field of breast cancer diagnosis, can solve the problem that the disease cannot be cured, and achieve the effect of low requirements, easy interpretation, and accurate results

Inactive Publication Date: 2017-03-08
北京鑫诺美迪基因检测技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oncologists pointed out that in recent years, the incidence of cancer in my country has shown an obvious upward trend, but less than half of the patients received standard treatment
Treatment of ER+/HER2- breast ca

Method used

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  • Breast cancer metastasis assessment kit
  • Breast cancer metastasis assessment kit
  • Breast cancer metastasis assessment kit

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0065] Prepare PCR amplification MIX, the specific components are as follows in Table 1:

[0066] Table 1 PCR amplification MIX components and dosage

[0067] components Dosage per person (μL) DNA polymerase 0.25 reverse transcriptase 0.25 dNTPs 2 Mg 2+

3～3.5 10×DNA polymerase buffer 2.5 5×reverse transcriptase buffer 5 UDG enzyme 0.1 dUTP 0.01

[0068] Prepare primers and probe nucleotide sequences according to SEQ ID NO.1~33, and label the fluorescent reporter group FAM at the 5' end of each probe's nucleotide sequence, and label the fluorescent quencher group BHQ1 at the 3' end, Prepare the PCR amplification reaction system, as shown in Table 2 below:

[0069] Table 2. Primer probe mixture

[0070]

[0071]

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PUM

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Abstract

The invention discloses a breast cancer metastasis assessment kit. The breast cancer metastasis assessment kit comprises specific fluorescent quantitative PCR detection primer pairs of total 11 target genes including BIRC5, DHCR7, UBE2C, AZGP1, IL6ST, MGP, RBBP8, STC2, CALM2, OAZ1 and RPL37A and preferably further comprises Taqman probes of the 11 target genes. By detecting the expression levels of the 11 target genes and combining pathological information such as the lymph node status and the tumor size of a breast cancer patient, the relapse and far-end metastasis risks of the breast cancer patient in 10 years can be assessed, the patient can adopt corresponding treatment measures, and excessive treatment is avoided. The kit is easy and convenient to operate, easy to interpret and low in requirement on instruments; in addition, due to the fact that a totally-closed mode is adopted in the whole PCR process, the cross contamination possibility is avoided, and then a result is more precise.

Description

technical field [0001] The invention relates to the technical field of breast cancer diagnosis, in particular to a breast cancer metastasis assessment kit, which is used for breast cancer recurrence risk assessment and distant metastasis assessment. Background technique [0002] In China, the health burden of cancer is increasing year by year. More than 1.6 million people are diagnosed with cancer and 1.2 million die of cancer every year. Like most other countries, breast cancer has become the most common cancer in Chinese women; the number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's annual breast cancer incidence, respectively. Oncologists pointed out that in recent years, the incidence of cancer in my country has shown an obvious upward trend, but less than half of the patients received standard treatment. ER+ / HER2- breast cancer is a subtype of breast cancer, accounting for about 2 / 3 of all breast cancers, and about 33,000 wom...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q1/6886C12Q2600/158C12Q2531/113C12Q2545/101C12Q2561/101
Inventor 王林海刘沛
Owner 北京鑫诺美迪基因检测技术有限公司
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