Method for rapid detection of single nucleotide polymorphism of sheep NELF gene by using PCR-SSCP and application thereof

Abstract

The invention discloses a method for rapid detection of the single nucleotide polymorphism of a sheep NELF gene by using PCR-SSCP and application thereof. According to the invention, the whole genome DNAs of a to-be-detected sheep are used as a template; a primer pair P is used as primers; PCR amplification is carried out so as to obtain a sheep NELF gene fragment; a PCR product undergoes denaturation so as to form single-stranded DNAs; and the single-stranded DNAs undergo electrophoresis in neutral polyacrylamide gel so as to produce different electrophoretic bands. The single nucleotide polymorphism of the site 454 of the sheep NELF gene is identified according to SSCP typing results. The method can rapidly detect the single nucleotide polymorphism of the sheep NELF gene and lays a foundation for establishment of a relationship between the single nucleotide polymorphism of the sheep NELF gene and reproductive performance, so basic data is provided for marker assisted selection of reproductive traits of Chinese sheep and rapid building of a sheep population with excellent genetic resources is facilitated.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the screening and detection of sheep single nucleotide polymorphism (SNP) molecular markers, in particular to the rapid detection of the single nucleotide polymorphism at the 454th position of the NELF genome by using the PCR-SSCP method. Specifically, polyacrylamide gel electrophoresis is performed on the denatured product obtained by PCR amplification, and then the single nucleotide polymorphism is determined according to the electrophoresis result. Background technique [0002] In the past 20 years, especially in the past 10 years, molecular genetics and molecular biotechnology have developed by leaps and bounds. Among them, molecular genetic markers are currently the most studied content in livestock breeding, and they are also the most likely to be obtained in livestock breeding in the near future. Widely applied research projects. Genetic markers refer to those genetic characte...

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Problems solved by technology

[0005] At present, domestic and foreign studies on NELF gene polymorphisms are mainly focused on model animals such as mice, while studies on domestic animal NELF gene single nucleotide polymorphisms have not been reported yet.

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