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Cunninghamella griseus and its culture method and inoculum and its application

A kind of technology of C. japonica and culture method, applied in the field of microorganisms, can solve the problems of unsuitable for non-point source pollution remediation, incomplete physical method remediation, high cost of chemical method, achieve high economic and social benefits, reduce toxicity, operation simple effect

Active Publication Date: 2019-09-03
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Physical and chemical methods can also repair chlorothalonil, but the physical method is not complete and further treatment is difficult; the chemical method is expensive and not suitable for the restoration of non-point source pollution

Method used

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  • Cunninghamella griseus and its culture method and inoculum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This example is used to illustrate the degradation performance of C. cinerea of ​​the present invention on chlorothalonil in water.

[0062] Inorganic salt liquid culture was sterilized at 121° C. for 15 minutes, and after cooling to room temperature, chlorothalonil pesticide was added thereto so that the content of chlorothalonil reached 20 mg / L.

[0063] Sterilize the fungal liquid culture medium at 115°C for 20 minutes, and after cooling to room temperature, cultivate the above-mentioned activated C. cinerea to the logarithmic phase with the sterilized fungal liquid medium; then take the logarithmic phase culture liquid, Centrifuge at 3000r / min for 5 minutes to obtain the dried fungus body of gray Xiaogram silvery mildew.

[0064] Weigh 0.5g of dry bacteria and add it to 10mL of the above-mentioned inorganic salt liquid medium containing chlorothalonil, so that the inoculation amount of silvery mildew can reach 50g dry weight / m 3 , and then cultured at 170rpm, 30±1°...

Embodiment 2

[0067] This example is used to illustrate the degradation performance of C. cinerea of ​​the present invention on chlorothalonil in soil.

[0068] Sterilize the fungal liquid medium at 115°C for 20 minutes, cool it to room temperature, insert the above-mentioned activated silvery mildew and use the sterilized fungal liquid medium to cultivate to the logarithmic phase; then take the logarithmic phase culture medium , Centrifuge at 3000r / min for 5min to obtain dry thallus of gray small gram silvery mildew.

[0069] Apply the above-mentioned dry bacteria obtained evenly to the soil with a chlorothalonil content of 10 mg / kg, so that the content of fungi in the soil reaches 9 g dry weight / m 3 . Cover the soil surface with porous plastic film to keep the soil temperature at 20-35°C. The soil was irrigated and plowed weekly so that the moisture content in the soil was maintained at 40-60% by weight. After 48 hours, the degradation rate of chlorothalonil was detected by gas chromat...

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Abstract

The invention relates to the field of microorganisms, and concretely relates to a Cunninghamella bertholletiae. The preservation number of the Cunninghamella bertholletiae is CGMCC No.11115. The invention also discloses a culture method of the Cunninghamella bertholletiae, and an inoculant containing the Cunninghamella bertholletiae. The invention further discloses an application of the Cunninghamella bertholletiae and the inoculant in degradation of chlorothalonil. The Cunninghamella bertholletiae can be used to carry out in-situ biological restoration of chlorothalonil polluted soil without destroying the soil environment of plant growth or generating secondary pollution, so in-situ processing, simple operation, high-efficiency degradation of chlorothalonil in soil in a short time and reduction of the toxicity of the chlorothalonil in environment are realized, the purposes of soil environment protection and food safety guaranteeing are reached, and the disadvantages of high interference to the original ecological system of the soil, possibly caused secondary pollution and large investment cost of physical and chemical restoration methods are overcome.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of silvery mildew, specifically, a strain of silvery mildew that has the function of degrading chlorothalonil, and the cultivation method of the silvery mildew contains the Inoculants of C. cinghamella as active ingredients and their application in degrading chlorothalonil. Background technique [0002] Chloratholonil, i.e. tetrachloroisophthalonitrile, is a high-efficiency broad-spectrum aromatic fungicide, which has been used in agricultural production for more than 40 years. Chlorothalonil has a good control effect on melon and fruit economic crop diseases caused by fungi, and there has been no report of drug resistance so far, so it has been widely used in the world. In addition to eliminating diseases that can prevent and control a variety of crops, bactericides can also be used as acaricides, nematicides, algicides, industrial fungicides and plant growth regulators. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34A62D3/02C12R1/645C02F101/38
Inventor 林森任立伟过钰梁王培红
Owner 粮华生物科技(北京)有限公司