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Stevia rebaudiana rooting culture method

A technology for rooting culture and rooting medium, which is applied in the field of stevia rooting culture, can solve the problems of easy infection, high technical requirements, slow rooting and the like, and achieves the effects of improving survival rate, simple process and improving rooting rate.

Inactive Publication Date: 2017-05-10
MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that the tissue culture seedlings just taken out of the tissue culture room are very slender and weak, and direct cuttings take root slowly and are prone to diseases. A little carelessness will cause a large number of dead seedlings, and the technical requirements for cutting

Method used

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Embodiment Construction

[0017] Below in conjunction with specific embodiment, further illustrate the present invention. These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.

[0018] Present embodiment relates to a kind of Stevia rebaudiana rooting culture method, comprises the steps:

[0019] (1) Selection of seedlings: Select the young branches of Stevia rebaudiana that grow vigorously, and cut them into single-node stems;

[0020] (2) Pretreatment: soaking the Stevia rebaudiana stems and branches in the rooting solution for 2-4 hours, wherein the rooting solution is a mixture of willow juice extract and aspirin solution;

[0021] (3) Rooting culture: inoculate the stevia stems and branches treated in step (2) in the rooting medium for rooting culture for 10~15 days, the culture temperature is 25~28°C, the light intensity is 2000Lux, and the light time is 12~ 13h / d, wherein, the rooting medium is 1 / 2MS medium as basic medi...

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Abstract

The invention relates to the technical field of agricultural planting, in particular to a stevia rebaudiana rooting culture method. The method includes following steps: seedling selection: selecting a stevia rebaudiana shoot which grows strongly, and cutting the shoot into a single-joint stem segment; pretreatment: soaking the single-joint stem segment in rooting liquid for 2-4h; rooting culture: inoculating the treated stevia rebaudiana shoot into a rooting culture medium for rooting culture, wherein culture temperature is 25-28 DEG C, illumination intensity is 2000Lux and illumination time is 12-13h / d. According to the method, the rooting liquid is used for soaking to promote rooting of the shoot, the shoot is then inoculated in the rooting culture medium, and rooting is promoted through double culture, so that rooting rate of the shoot is increased greatly; the shoot after rooting is subjected to cuttage cultivation, so that survival rate of seedlings is increased; the method is simple in process, convenient to operate and high in application value.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a rooting culture method of Stevia rebaudiana. Background technique [0002] Stevia is a perennial herbaceous plant of Compositae, native to subtropical South America. The roots, stems and leaves of Stevia rebaudiana contain steviol glycosides. Stevioside has the characteristics of high sweetness (200-300 times that of sucrose), low calorie (only 1 / 300 of sucrose), and can increase blood sugar, lower blood pressure, and promote metabolism. Heart disease, dental caries, etc. have a certain auxiliary effect, so it has increasingly attracted people's attention and attention, and has become the "third sugar source" after sucrose and synthetic sugar. [0003] In recent years, with the increase in demand for stevia, the planting area is also expanding. It is planted in Hainan in the south, Heilongjiang in the north, east coast in the east, and Tibet in the west. At pre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 贾德军
Owner MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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