40 results about "Stevia serrata" patented technology

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

The invention discloses a technological process for preparing stevioside from stevia rebaudiana bertoni. The technological process comprises the following steps: carrying out countercurrent extraction on the dried stevia rebaudiana bertoni raw material with water, sequentially adsorbing the obtained extracting solution with macroporous absorption resin columns which are connected in series, allowing the residual liquid after absorption to enter a countercurrent extraction device for cyclic extraction, reabsorbing the obtained extracting solution with the resin columns which are connected in series once again, and completing the extraction separation closed cycle process; after the whole countercurrent extraction and absorption process is completed, eluting the resin columns with water for removing impurities, desorbing with stripping liquid, collecting the stripping liquid, concentrating and drying to obtain the crude extract of the stevioside; and refining the crude extract by a solvent method and decolorizing resin respectively to finally obtain the stevioside product. In the obtained product, the content of the stevioside compound is 70-100%, wherein, two components such as the stevioside and rebaudioside account for 70-90% of the whole stevioside compound, thus meeting the standards for a medicine, a healthcare product or food.

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w/w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w/w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

The invention relates to a microorganism-containing organic compound fertilizer for stevia rebaudiana, which is characterized by comprising the following components in parts by weight: 5-8 parts of natural nitrogen fertilizers with humic acid, 3-5 parts of phosphatic fertilizers, 6-7 parts of potash fertilizers, 2-3 parts of monopotassium phosphate, 3-5 parts of saccharin sodium solution, 25-30 parts of polymer water-absorbent resins and 60-72 parts of bioorganic fertilizers. The natural nitrogen fertilizers with humic acid comprise 2-3 parts of nitrogen fertilizers, 0.5-1 part of phosphatic fertilizers, 2-3 parts of potash fertilizers and 93.5-95.5 parts of humic acid by weight. The saccharin sodium solution contains saccharin sodium at the concentration of 0.4-0.6%. Compared with the chemical fertilizers, the organic compound fertilizer is green, nontoxic and harmless, has no side effect, resists drought, preserves water and fertility, keeps seedlings, improves the soil never to be hardened, regulates the pH value of the soil to be neutral, does not pollute ecological environment, prevents diseases and insect pests, reduces the usage amount of the pesticides by 50%, lowers the cost by 15-20%, increases the sugar degree by 2-3, increases the yield and benefit by more than 15-25% and ensures the seedling survival rate by more than 95%.

The invention belongs to the technology of plant tissue culture and particularly relates to a cuttage method of stevia rebaudiana rootless tissue culture seedling. The existing stevia rebaudiana tissue culture rapid propagation technology has the following defects: the growth of the test-tube seedling is limited by the environmental conditions, the seedling is very slim and fragile, and the survival rate of cuttage is extremely low. The method of the invention comprises the following steps: a cuttage seedling is cut to 3-4cm, two basal leaves are removed, the leaf nodes are retained; a float tray is used, fine sand is filled in a planting hole, a hole is digged, the seedling is inserted; a support is arranged, a plastic film is covered; the float tray is directly placed in the open air in spring. When the temperature is high in summer, the float tray is covered by a sunshading net; ventilation is performed for hardening-seedling 10-15 days after planting; the plastic film is removed after 2-3 days; the sunshading net is removed after 1-2 days; liquid fertilizer is poured; and the strong seedling with numerous roots is cultured 20-25 days after cuttage. The invention has the following advantages: the production period is shortened, the consumed materials are reduced, the use of the device is saved, the utilization efficiency of the tissue culture room is increased, the energy is saved, the technical effect is good, and the transplanting survival rate is up to 99%.

The invention discloses a method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents, which comprises the following steps of: selecting varieties at an asexual stage, preparing seeds of generation F1 by cross breeding at a sexual stage, and preparing seeds of generation F2 by using a backcross method. The method of the invention has the advantages of high leaf yield, high total glycoside content, high Rebaudioside-A (RA) glycoside content, high resistance and stable properties.

The invention relates to the technical field of agricultural planting, in particular to a stevia rebaudiana rooting culture method. The method includes following steps: seedling selection: selecting a stevia rebaudiana shoot which grows strongly, and cutting the shoot into a single-joint stem segment; pretreatment: soaking the single-joint stem segment in rooting liquid for 2-4h; rooting culture: inoculating the treated stevia rebaudiana shoot into a rooting culture medium for rooting culture, wherein culture temperature is 25-28 DEG C, illumination intensity is 2000Lux and illumination time is 12-13h/d. According to the method, the rooting liquid is used for soaking to promote rooting of the shoot, the shoot is then inoculated in the rooting culture medium, and rooting is promoted through double culture, so that rooting rate of the shoot is increased greatly; the shoot after rooting is subjected to cuttage cultivation, so that survival rate of seedlings is increased; the method is simple in process, convenient to operate and high in application value.

The invention relates to a method for rapid preparation of leaf protoplasts of Stevia rebaudiana. According to the invention, optimal conditions for separating the leaf protoplasts of Stevia rebaudiana are screened out with small and tender leaves of tissue culture seedlings of Stevia rebaudiana as a material by controlling factors like plasmolysis time, the ratio and concentration of cellulase Onozuka R-10, hemicellulase and macerozyme R-10, the concentration of mannitol in enzymatic hydrolysis liquid, the pH value of the enzymatic hydrolysis liquid, hydrolysis time, etc. Free protoplasts prepared by using the method are great in quantity and high in activity; and the method is simple and fast in preparation of the leaf protoplasts of Stevia rebaudiana.

The invention provides a disease-resistant compound fertilizer for stevia rebaudiana and a preparation method thereof. The disease-resistant compound fertilizer comprises the following components in parts by weight: 50-60 parts of human and animal excreta, 20-30 parts of milk vetch straws, 10-12 parts of cane sugar residues, 10-12 parts of yellow ginger residues, 10-12 parts of yeast residues, 12-15 parts of tomato residues, 8-10 parts of modified bentonite, 8-10 parts of diatomite, 1-2 parts of flos daturae, 3-4 parts of isatis root, 2-3 parts of the fruit of Rangoon creeper, 2-3 parts of polygonum cuspidatum, 2-3 parts of folium isatidis, 2-3 parts of fructus forsythia, 2-3 parts of rhizoma phragmitis, 2-4 parts of houttuynia cordata, 6-8 parts of urea, 2-4 parts of phosphate fertilizers and 4-6 parts of potassic fertilizers. The compound fertilizer is prepared by the steps: extracting, fermenting, and mixing, thereby obtaining the product. According to the compound fertilizer disclosed by the invention, due to scientific arrangement, the needed nutrients of stevia rebaudiana growth are guaranteed, multiple anti-virus traditional Chinese medicinal materials are effectively extracted, the disease resistance of the stevia rebaudiana can be increased, and vigorous growth of the stevia rebaudiana is promoted.

The invention provides a stevia rebaudiana seed preservation method which comprises the following steps: (1) collecting stevia rebaudiana seeds, and naturally sun-curing to make the water content be 3-5%; and (2) wrapping the stevia rebaudiana seeds with gauze, placing the gauze wrapping the stevia rebaudiana seeds and a moisture-proof agent in a glass vessel, sealing, placing the glass vessel in a ventilation environment with the environment temperature of 8-12 DEG C, and preserving. The stevia rebaudiana seed preservation method can effectively prevent moisture and air from entering the seeds, slows down the respiration of the seeds, thereby delaying the inactivation time of the seeds and prolonging the preservation life of the seeds.

The invention discloses an application of stevia rebaudiana slag in cultivating edible fungi. The stevia rebaudiana slag serves as the raw material of cultivation material. The stevia rebaudiana slag has the advantages of lowering production cost and improving production benefit, considering the discharge of the waste stevia rebaudiana slag produced in the relevant stevia rebaudiana slag industry and solving the problem of environmental pollution.

The invention relates to rosaroxbunghii and dianthus chinensis tea and a making method for the same. Rosaroxbunghii is used as a raw material. The rosaroxbunghii and dianthus chinensis tea is characterized in that dianthus chinensis and stevia are also used as raw materials. The making method comprises the steps of (1) fruit selection and drying of rosaroxbunghii; (2) preparation of rosaroxbunghii particles; (3) screening and drying of dianthus chinensis and stevia; (4) preparation of dianthus chinensis tea particles and stevia particles; and (5) uniform mixing and packaging. According to the rosaroxbunghii and dianthus chinensis tea and the making method for the same, the formula is unique, reasonable compatibility of medicines is ensured, the nature of medicaments and the taste of tea supplement each other, blood sugar, blood lipid and blood pressure can be reduced, inflammation can be diminished, the rosaroxbunghii and dianthus chinensis tea has the healthcare effects of body nourishing and strengthening, stomach reinforcement, gut purging, constipation remission and the like, can be drunk by general populations, and is particularly suitable for diabetics and patients suffering from gastroenteritis, hyperglycemia, hypertension, hyperlipidemia and constipation, the types of the tea are rich, the making method is convenient and easy, great amounts of active ingredients are dissolved out, and the active ingredients can be easily absorbed by a human body after the tea is drunk.

The invention provides an insecticide for stevia rebaudiana. The insecticide is prepared from raw materials in parts by weight as follows: 20-25 parts of pyrethrum, 5-8 parts of tobacco stems, 25-30 parts of nerium oleander, 12-16 parts of macleaya cordata, 10-15 parts of atropa belladonna, 8-14 parts of lycoris radiate, 8-12 parts of jequirity beans, 13-17 parts of wisteria, 15-20 parts of stemona, 12-18 parts of radix aconite and 18-26 parts of phytolacca acinosa. The insecticide is obtained by combining the raw materials after the raw materials are extracted. The insecticide for stevia rebaudiana is prepared from plant raw materials, has the advantages of being green and safe, free from causing pesticide resistance, low in environment pollution and the like and has a good control effect on insect damage of stevia rebaudiana.

The invention relates to the technical field of agricultural cultivation, particularly to a liquid fertilizer for promoting rooting of stevia rebaudiana and a preparation method. The fertilizer comprises the following components: a phosphatic fertilizer, potassium sulfate, urea, a micronutrient fertilizer, willow shoots, poplar shoots, setaria viridis, white sugar, rice vinegar, aspirin and vitamins. The preparation method comprises the steps of weighing the willow shoots, the poplar shoots and setaria viridis, putting the willow shoots, the poplar shoots and setaria viridis into a container to be mashed, and adding water to soak the mashed materials; filtering the mixture to obtain a soaking solution; weighing the phosphatic fertilizer, potassium sulfate, urea and the micronutrient fertilizer, adding the phosphatic fertilizer, potassium sulfate, urea and the micronutrient fertilizer into a container with water, and stirring the materials to dissolve the materials; weighing white sugar, aspirin and vitamin B12, adding white sugar, aspirin and vitamin B12 into a container with water, stirring the materials to dissolve the materials; and mixing rice vinegar with the soaking solution and dissolving solutions, and carrying out concentration to obtain the liquid fertilizer. The liquid fertilizer can provide rich nutrients for growth of stevia rebaudiana and can achieve a relatively good rooting effect. The preparation method of the fertilizer is simple and has relatively high application value.

The invention belongs to the technical field of agricultural planting, and particularly relates to a stevia rebaudiana seed cold-resistant agent, comprising the following components in parts by weight: 4-12 parts of paclobutrazol, 8-20 parts of chitosan, 10-16 parts of humic acid, 4-6 parts of nitric acid, 10-20 parts of rapeseed oil, 6-10 parts of a compound emulsifier, 6-10 parts of a compound antifreeze and 600-800 parts of distilled water. The stevia rebaudiana seed cold-resistant agent provided by the invention has the following beneficial effects: after seed soaking treatment with the paclobutrazol, chitosan, humic acid, nitric acid, rapeseed oil, compound emulsifier and compound antifreeze, the resistance and growing ability of stevia rebaudiana seeds under a cold environment are obviously improved.

The invention specifically relates to a fertilizer for Stevia rebaudiana, belonging to the technical field of agricultural plantation. The fertilizer comprises, by weight, 200 to 300 parts of urea, 10 to 20 parts of zinc sulfate, 20 to 30 parts of potassium phosphate, 10 to 20 parts of apple pomace, 5 to 15 parts of dried chicken manure, 30 to 40 parts of vinasse, 10 to 20 parts of cassava residues, 5 to 15 parts of plant ash, 10 to 20 parts of shell powder and 5 to 15 parts of silkworm excrement. According to the invention, nutrient elements are reasonably proportioned; after application of the fertilizer, nutritional demands of Stevia rebaudiana are met, the utilization rate of the fertilizer is increased, environmental pollution caused by loss of the fertilizer is alleviated, and sustainable development of an agricultural ecological system is benefited; and the fertilizer has high economic benefits and promotion value and exerts long-acting and stable effect.

The invention discloses a fertilizer capable of controlling the damping-off of stevia rebaudiana, and relates to the technical field of stevia rebaudiana plantation. The fertilizer comprises the following components in parts by weight: 150 to 200 parts of plant ash, 100 to 120 parts of urea, 100 to 120 parts of cotton seed shell and rapeseed shell, 70 to 90 parts of straw, 70 to 90 parts of tea dregs, 60 to 70 parts of cowpea vine, 20 to 40 parts of fish bone, 30 to 40 parts of sheep manure, 70 to 90 parts of nutritional liquid, 50 to 60 parts of Chinese herbal additive, and 50 to 60 parts of bactericide. The fertilizer can supply elements required for the growth of stevia rebaudiana and can also prevent and treat damping-off of stevia rebaudiana.

The invention provides a cultivation method for a stevia rebaudiana tetraploid. The cultivation method comprises the following steps: seed selection; seed treatment: a step of subjecting stevia rebaudiana seeds to cleaning and disinfection, then cutting the stevia rebaudiana seeds open in an aseptic culture medium vessel, taking embryos out of the aseptic culture medium vessel, carrying out aseptic culturing, and allowing the embryos to germinate into green embryo protocorms; and inducing culturing: a step of inoculating protocorms on a budding inducing culture medium added with dimethyl sulfoxide and colchicine, carrying out first inducing culturing, then carrying out transferring to a culture medium only containing colchicine, carrying out secondary inducing culturing, then carrying out transferring to a culture medium without dimethyl sulfoxide and colchicine, and carrying out culturing so as to obtain a stevia rebaudiana tetraploid plant. With the cultivation method provided by the invention, the cultivated stevia rebaudiana tetraploid plant has doubled chromosome, so stevia rebaudiana has high medicinal ingredients; the yield of stevioside can be greatly improved; meanwhile, the cultivation method has the advantages of simplicity, stability, environmental friendliness, applicability to industrial production, and extensive application prospects.

The invention discloses organic fertilizer for stevia rebaudiana. The organic fertilizer is prepared from the following raw materials in parts by weight: 30 to 35 parts of stevia rebaudiana residues, 8 to 10 parts of clay, 15 to 20 parts of nitrogen fertilizer, 10 to 15 parts of phosphorus fertilizer, 15 to 22 parts of potassium fertilizer, 3 to 8 parts of a medium trace element, 5 to 10 parts of palygorskite and 5 to 7 parts of a super absorbent polymer. The organic fertilizer is pollution-free, and contains multiple trace elements, and the growth requirement of the stevia rebaudiana can be met.

The invention belongs to the technical field of agricultural planting, and particularly relates to a biological organic fertilizer for stevia rebaudiana. The biological organic fertilizer is prepared from, by weight, 30-50 parts of animal waste, 10-20 parts of humus, 20-30 parts of abamectin raw dregs, 10-20 parts of straw, 6-10 parts of derris roots, 6-10 parts of pear dregs, 4-6 parts of mineral substances and 3-5 parts of plant ash. The ratios of nutrient elements are reasonably matched, after the biological organic fertilizer is applied, the problems of nutritional requirements of stevia rebaudiana are solved, moreover, the utilization rate of the fertilizer is improved, the environmental pollution caused by fertilizer loss is reduced, and the biological organic fertilizer has the effect of improving soil at the same time.

The invention belongs to the technical field of agricultural planting and particularly relates to a compound fertilizer used for stevia rebaudiana. The compound fertilizer includes, by weight, 60-100 parts of urea, 20-30 parts of potassium phosphate, 4-8 parts of zinc sulfate, 4-8 parts of copper sulfate, 20-30 parts of humus, 5-15 parts of citric acid, 10-30 parts of pear residue, 10-30 parts of apple residue, 10-30 parts of honeysuckle residue, and 10-30 parts of mushroom residue. The compound fertilizer has a reasonable formula of nutrients, and can solve the problem of nutritional demands of the stevia rebaudiana when being applied and is increased in utilization rate, thereby reducing environment pollution due to fertilizer loss. The compound fertilizer is beneficial to sustainable development of agricultural ecological systems and has high economic benefit and promoting value, and also has the effects of improving soil and increasing yield of the stevia rebaudiana.

The invention provides stevia rebaudiana stump seedling-raising nutritive water which comprises the following components in parts by weight: 0.6-1.0 part of urea, 0.6-1.0 part of monopotassium phosphate, 0.02-0.05 part of boric acid, 6-9 parts of sargassum powder aqueous extract, 4-7 parts of astragalus water extract, 3-5 parts of panax notoginseng homogenate, 0.2-0.3 part of luteolin, 0.3-0.6 part of oryzanol and 900-1100 parts of water. The preparation method comprises the following steps: dissolving the various raw materials, and uniformly mixing, thereby obtaining the nutritive water. According to the stevia rebaudiana stump seedling-raising nutritive water disclosed by the invention, the transplanting survival rate of stevia rebaudiana stumps can be effectively improved, the operation of filling the gaps with seedlings after transplanting of the conventional stevia rebaudiana stumps is avoided, the burdens of farmer households are alleviated, and the breeding amount of the stevia rebaudiana seedlings is increased.

The invention discloses a crude extraction technology of stevioside of stevia rebaudiana. The technology comprises the following steps: step one, cutting off leaves from stevia rebaudiana, and chopping the leaves; step two, mixing water and critic acid to prepare a critic acid solution with a mass percentage of 0.35%, and pouring the prepared critic acid solution into a container; step three, soaking the processed stevia rebaudiana leaves in the critic acid solution at a room temperature of 10 to 28 DEG C, stirring the solution by hands to evenly mixing the leaves and the critic acid solution, soaking for 15 to 20 minutes, and collecting the liquid; step four, repeating step three for one or two times, and merging the obtained liquids; step five, filtering the liquid by a filter screen to obtain the filtrate. Compared with the prior art, the provided crude extraction technology has the advantages that only critic acid is used to complete the crude extraction, the steps are simple, the cost is low, the extraction yield is high, moreover the obtained filtrate is clean, and the post treatment becomes easier.