65 results about "Arabidopside E" patented technology

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

The invention discloses a preparing method of sweet chrysanthemum glycosides and chromocor extract from sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside], derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; counting 5-100%(w / w) of each sweet chrysanthemum glycosides element and chromocor element content in sweet chrysanthemum glycosides and chromocor extract; setting the chromocor element sum in sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon-7-0-beta-D glycosides, quercetin, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] at 5-100%(w / w).

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w / w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w / w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

The invention provides a preparation method of flavonoid glycosides in scutellaria baicalensis. The method is suitable for quickly preparing four flavonoid glycosides comprising baicalin, wogonoside, chrysin-6-C-alpha-L-Arabic glucoside-8-C-beta-D-glucoside and oroxylinA-7-O-glucuronide. The preparation method comprises the following steps of: pulverizing scutellaria baicalensis and then adding water to extract; sequentially carrying out alcohol precipitation and high-speed centrifugation; after membrane separation, loading nonpolar macroporous absorption resin and eluting by alcohol / water; collecting eluent and concentrating and drying to obtain a scutellaria baicalensis macroporous resin component; and obtaining a scutellaria baicalensis effective component through parallel preparing liquid-phase chromatographic separation by using acetonitrile water as a flow phase. The preparation method has good selectivity and high purity of the obtained compound and greatly improves the separation and purification efficiency. The preparation process has high repeatability and good maneuverability, is easy to realize standardization and industrialization and has certain guidance effects on quickly obtaining flavones active constituents in the scutellaria baicalensis.

The invention discloses common lophatherum herb extract and a preparation method and application thereof. The common lophatherum herb extract comprises isovitexin, swertiajaponin, swertisin, luteolin 7-O-beta-D-glucose-6-C-alpha-L-arabinoside, orientin and vitexin. The preparation method for the common lophatherum herb extract comprises the following steps of: extracting the dry medicinal material of common lophatherum herb by using ethanol aqueous solution to obtain crude extract, and purifying by using a macroporous adsorption resin column. The extract can be used for preparing a medicine or health care product for resisting respiratory syncytial virus, parainfluenza virus type 3 or influenza A virus.

This invention involves a kind of enzymatic production method of xylose. The specific steps are pre-treat wasted crop to produce xylan or steam exploded substance; then add xylanase, bi-activity xylose / arabinosidase and alpha-glucuronidase to buffer to generate multi- enzyme mix liquid. Mixing it and adding the xylan or steam exploded substance to multi- enzyme mix liquid to perform enzymatic hydrolysis. After hydrolysis, adds ethanol to enzymatic hydrolysis liquid and further condese and crystallize to get xylose product. The release rate of xylose can reach as much as two times of using Xylanase and xylosidase so to increase the productivity. The power consuming is low. The product can keep the natural quality. At the same time the xylose produced with enzyme has no harm to microbial species, and can be used directly in biotransformation of xylitol by yeast. It is virus free and with good fermentation performance. It is benefical for the transformation from xylose to xylitol.

The invention provides a method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials, which synchronously measures the contents of at least two of five ingredients of ellagic acid, 3,3'-dimethoxy ellagic acid, 3,3'-dimethoxy ellagic acid-4'-O-beta-D-xyloside, 3,3'-dimethoxy ellagic acid-4'-O-beta-D-glucoside and 3,3'-dimethoxy ellagic acid-4'-O-alpha-D-arabinoside contained in the euscaphis japonica medicinal materials by using a high efficiency liquid chromatography or an ultra-high efficiency liquid chromatography under the same chromatogram condition. In the invention, the contents of the multiple ingredients in the euscaphis japonica medicinal materials are measured under the same chromatogram condition so that the method for measuring the content of the ellagic acid ingredient in the euscaphis japonica medicinal materials has better integrity, characteristics and stability as well as high precision, favorable repeatability and high recovery rate and capability of effectively controlling the quality of the euscaphis japonica medicinal materials, thereby ensuring the effectiveness and the safety of clinical medication and laying the foundation for redevelopment and application of the euscaphis japonica medicinal materials.

The invention relates to a total flavone c-glycoside effective component extract separated from traditional Chinese medicine abrus mollis hance, belonging to natural medicine technical field, which mainly contains apigenin-6, 8-bis-C-glucoside, apigenin-6-C-glucose-8-C-arabinfuranoside and apigenin-6-C-arabinose-8-C-glucoside, whose flavone c-glycoside content is higher than 40%. The invention relates to an extraction method of the total flavone c-glycoside effective component extract. Pharmacodynamic tests prove that the abrus mollis hance total flavone c-glycoside extract can be used as active component to prepare the drugs for the prevention and treatment of acute and chronic hepatitis or the like.

The invention belongs to the field of biological engineering and provides a novel bifunctional enzyme RuXyn1 with low-temperature resistance and activities of beta-D-xylosidase and alpha-L-arabinofuranosidase. The RuXyn1 has an amino acid coding sequence shown as SEQ ID No 2 and has a nucleotide coding sequence shown as SEQ ID No 1. The RuXyn1 can be prepared by a genetic engineering method or an artificial synthesis method. The RuXyn1 has functions of the arabinofuranosidase and the xylosidase, can keep higher activity in a low-temperature environment, and can be applied to the application fields of cellulose bioconversion, chemical industry, textiles, foods, bioenergy, feed additives, pharmaceutical industry and the like.

A process of preparing compound sugar by a-L-Arab carbohydrase. First, prepare solution of wood polyose. Then, add xylanase and a-L-Arab carbohydrase into the solution. Finally, product is obtained after decolour, iron exchange and enrichment from hydrolytic solution.

It is an object of the present invention to find out a novel gene marker by which a drug-resistant cancer cell can be detected and provide a means of efficiently and comprehensively detecting a drug-resistant cancer cell using this marker. In the present invention, gene amplifications or deletions have been analyzed in cancer cell strains resistant to drugs, which are anticancer drugs having particularly serious side effects and being administered to cancer patients at a high frequency (namely, camptothecins, cisplatins, etoposides, adriamycins (ADM), and cytosine arabinosides), and parent cancer cell strains. As a result, it was found out that the acquisition of drug-resistance to an anticancer drug in a test cancer cell can be detected by detecting amplification of one or more genes selected from ABC transporter genes and BCL2 family genes consisting of ABCA3 gene, ABCB6 gene, ABCB8 gene, ABCB10 gene, ABCC4 gene, ABCC9 gene, ABCD3 gene, ABCD4 gene, ABCE1 gene, ABCF2 gene, BCL2L2, BCL2L10, BCL2L1, and BCL2A1 which are novel gene markers relating to the acquisition of drug resistance of cancer cells.

The invention discloses a content measurement method of abrus herb capsules. In the method, a high performance liquid chromatography-ultraviolet (HPLC-UV) method is used for measuring the contents of apigenin-6,8-di-C-glucoside, apigenin-6-C-arabinose-8-C-glucoside and apigenin-6-C-glucose-8-C-arabinoside in abrus mollis hance (monarch drug in a prescription) in the abrus herb capsules under the same chromatographic condition. The method comprises the following steps: preparing a test sample solution; preparing a control sample solution; and measuring by gradient elution. The method has the advantages of strong specificity and better accuracy, precision and reproducibility, is simple and easy to operate, and has important significance for further perfecting the quality standards of the abrus herb capsules.

The invention discloses a blackberry extract. The blackberry extract comprises components in percentage by mass as follows: 58.3%-72.5% of cyanidin-3-glucoside, 11.6%-15.8% of cyanidin-3-xyloside, 9.8%-14.6% of cyanidin-3-oxalyl glucoside, 3.1%-6.4% of cyanidin-3-malonyl glucoside, 2.7%-4.1% of delphinidin-3-xylose and 0.3%-0.8% of cyanidin-3-arabinfuranoside. The extraction steps are as follows: after enzymolysis of blackberry fresh fruits by pepsase and trypsin sequentially, centrifugal separation is performed, a liquid supernatant is obtained and filtered by an ultrafiltration membrane, ingredients with molecular weight larger than 10 kDa are cut off, filtrate is subjected to vacuum freezing drying, and the blackberry extract is obtained. The invention further discloses an application of the blackberry extract in preparation of a liver cell oxidative damage inhibitor.

The invention relates to application of hederagenin-3-O-arabinopyranoside (hederagenin-3-O-arabinopyranoside). The Hederagenin-3-O-arabinopyranoside is obtained by utilizing a chemical extraction separation method. The chemical compound structure of the hederagenin-3-O-arabinopyranoside is identified by utilizing physical constants and spectroscopy methods to carry out an antineoplastic pharmacological testing for the hederagenin-3-O-arabinopyranoside, a result shows that the hederagenin-3-O-arabinopyranoside has good effect on antineoplastic aspects ,especially has good effects on restraining human breast cancer cells, sarcoma cells, gastric carcinoma cells, hepatoma carcinoma cells and prostate cancer cells. The hederagenin-3-O-arabinopyranoside can be used for preparing medicines for curing breast cancer, solid tumor, gastric cancer, liver cancer and prostate cancer.

The invention discloses a ferment preparation method for improving the flavor of ferment through an enzyme preparation. The method includes the steps that compound enzyme 1 is used for hydrolysis in the ferment fermentation process, and compound enzyme 2 is used for hydrolysis in the after-ripening process. The method is characterized in that the compound enzyme 1 used in the ferment fermentation process comprises cellulase, ligninase, hemicellulase, amylase and the like, and the concentration of the compound enzyme 1 is 100-2000 u / kg; the compound enzyme 2 used in the ferment after-ripening process comprises glucosidase, arabinosidase, rhamnosidase, galactosidase and the like, and the using amount of the enzyme is 50-450 u / L. Compared with the prior art, the method has the outstanding advantages that the compound enzyme 1 is used for promoting release of flavor precursor substances existing ferment raw materials in a glucoside mode and other nutrient substances, the compound enzyme 2 is used for prompting the situation that the favor precursor substances are hydrolyzed into fragrance substances, and therefore ferment food rich in compound fragrance and fermentation alcohol aroma, sweet in aftertaste, coordinative in favor, thick in texture, pure in taste, smooth in swallowing, stable in texture, safe, hygienic and uniform in luster can be obtained.

The invention discloses a method for preparing a triterpene compound oleanolic acid 3-O-[beta-D-glucopyranose-(1->3)]-beta-D-galactopyranose-(1->2)-alpha-L-pyrane glucosidase arabinofuranoside (Glycoside St-E2) and application of the triterpene compound. The triterpene compound is separated from acanthopanax plants. The method and the application have the advantages that the method includes simple steps and is high in extraction efficiency and product purity; as discovered in mouse preadipocyte culture tests, adipogenesis can be obviously inhibited by the triterpene compound by the aid of AMPK-PPAR gamma-C / EBP alpha mechanisms, and accordingly the triterpene compound is an effective medicine for preventing and improving obesity.

The invention discloses an application of arabinosidase in preparing wheat bran dietary fiber as well as wheat bran flour and wheat bran steamed buns, which are prepared from the arabinosidase. The application method comprises the following steps of: (1) preparing an enzyme solution of arabinosidase; (2) performing ultra-fine crushing on wheat bran; (3) adding the enzyme solution to the crushed wheat bran, and performing hydrolysis; and (4) after hydrolysis, performing heating, performing boiling-over and performing cooling to room temperature so as to obtain wheat bran dietary fiber. The method is simple and convenient; by using the arabinosidase or the combined action of the arabinosidase and xylanase and ferulic acid esterase, the wheat bran is directly hydrolyzed to obtain the wheat bran dietary fiber; the obtained wheat bran dietary fiber is higher in water holding capacity, oil holding capacity and oxidation resistance; besides, the gluten network structure in the prepared wheatbran flour fully absorbs water and the gluten structure is more stable; and the prepared wheat bran steamed buns not only contain rich wheat dietary fiber and vitamins, but also are good in leaveningproperties, loose and soft in structure, good in palatability, nutritional, healthy, and unique in wheat taste.

The present invention is efficient arabinosidase gene expressing process and belongs to the field of microbial gene engineering. The present invention provides arabinosidase gene expressing process, which includes inserting arabinosidase coding gene into vector pHsh to constitute recombinant plasmid, introducing the recombinant plasmid into colibacillus as host cell to constitute recombinant colibacillus, culturing the recombinant colibacillus to OD600 of 0.5-1.2, and final heat activated expression to obtain recombinant arabinosidase. The present invention reforms gene directively to raise its expression level. The recombinant arabinosidase has high stability, capacity of degrading lateral arabinose branch in xylanase with high polymerization degree, high resistance to environment factor, and relatively wide temperature range and pH range of catalytic activity. The present invention may be used in arabinosidase production for food, feed and papermaking.

The invention discloses a separation and purification method of malvidin-3-O-glucosidase arabinofuranoside and application of the malvidin-3-O-glucosidase arabinofuranoside in preparing an alpha-glucosidase inhibitor. The preparation and purification method comprises the steps of coarse extraction, macroporous resin adsorption, high-speed countercurrent chromatographic separation and solid-phase extraction and purification. The separation and purification method is characterized by combining high-speed countercurrent chromatography with a solid-phase extraction technology, and then optimizingtechnological parameters, thus separating a complicated blueberry raw material of an anthocyanin composition, thus preparing a high-purity malvidin-3-O-glucosidase arabinofuranoside monomer. An activity experiment discovers that the malvidin-3-O-glucosidase arabinofuranoside monomer is capable of remarkably inhibiting the activity of alpha-glucosidase and can be used for preparing the alpha-glucosidase inhibitor.

The invention discloses a method for producing multiple kinds of monosaccharide through bamboo processing residues. According the method, the bamboo processing residues are pretreated through cooking in a sulfate process with the alkali dosage being 2% to 10%, defibrination, washing, and saccharification achieved through a special enzyme preparation are conducted on the pretreated materials, and then glucose, xylose and arabinose are efficiently produced. The special enzyme preparation comprises 5-45 FPU / g cellulose, 3-30 U / g beta-cellulose glucosidase, 15-150 U / g cellulose xylanase and 0.3-2.1 U / g cellulose arabinosidase. According to the method, the bamboo processing residues are pretreated through cooking in the sulfate process with low alkali dosage, beta-aryl ether bonds in lignin are destroyed, and part of the lignin is removed; pretreatment of fiber defibering and devillicate brooming are conducted on the pretreated materials which are cooked in the sulfate process with low alkali dosage in a disk mill, effective separation of the cellulose, hemicellulose and the lignin is further achieved, and the accessibility of the cellulose and the hemicellulose to enzymes is improved; then, under the saccharification action of the special enzyme preparation, the materials are efficiently hydrolyzed into the glucose, the xylose and the arabinose.

The invention relates to application of an apple residue and apple leaf extract to prevention and control of phytopathogen. The apple residue and apple leaf extract contains polyphenols of phlorizin,quercitrin, isoquercitrin, quercetin-3-D-xyloside and quercetin-3-D-glucosidase arabinofuranoside, and can be applied to prevention and control of diseases caused by fungi and diseases caused by bacteria for various grain crops, economic crops, vegetables, fruit trees, melons and the like. A biological sterilizing preparation prepared by extracting a mixture or a single substance is environmentally friendly, is safe to human beings and livestock, and can be used for effectively preventing and controlling various plant diseases.

The invention relates to the natural pharmaceutical chemistry field and the technical field of biopesticides, and discloses a new alkaloid compound, a preparation method thereof and application in preparing antimicrobial pesticides. The alkaloid compound has a structure as shown in a drawing of the abstract, and has a chemical name of dihydronarciclasine-4-O-alpha-L-arabinopyranoside. The preparation method is characterized by comprising the following steps: by taking Amaryllidaceae plants as raw materials, extracting by methyl alcohol, ethyl alcohol or water-containing alcohol, filtering, concentrating to a small volume, dispersing by using water, then performing macroporous resin column chromatography, collecting at a part eluted by 30 percent of the ethyl alcohol, and then performing column chromatography repeatedly with column chromatography filler being silica gel or reverse phase silica gel, thus obtaining a new alkaloid. The Amaryllidaceae plants are Zephyranthescandida, Lycorisradiata, Lycorislongituba and Lycorisaurea. As a natural plant bactericide, the compound has remarkable effects of resisting Magnaporthe orythae and Verticillium dahliae, can be singly used, and also can be compounded with other bactericides to be prepared into a compounded bactericidal pesticide.

The invention discloses a preparation method of alkyl arabinoside, which is characterized in that the method comprises the following steps: firstly, mixing fully-acetylized arabinose and nonanol or decatyl alcohol with a solvent; then, synthesizing the fully-acetylated alkyl arabinoside under the effect of glycosidation catalysts; and next, obtaining the alkyl arabinoside through deacylation reaction. The invention has the advantages of simple process, convenient operation, high yield, low price and easy acquisition of the synthesis use materials, low production cost and no environment pollution, and in addition, the product has the advantages of good surface activity, no peculiar smell and stable performance, and belongs to a surface active agent with wide application prospects.

The invention relates to a total flavone c-glycoside effective component extract separated from traditional Chinese medicine abrus mollis hance, belonging to natural medicine technical field, which mainly contains apigenin-6, 8-bis-C-glucoside, apigenin-6-C-glucose-8-C-arabinfuranoside and apigenin-6-C-arabinose-8-C-glucoside, whose flavone c-glycoside content is higher than 40%. The invention relates to an extraction method of the total flavone c-glycoside effective component extract. Pharmacodynamic tests prove that the abrus mollis hance total flavone c-glycoside extract can be used as active component to prepare the drugs for the prevention and treatment of acute and chronic hepatitis or the like.

The invention relates to extractive of chickweed flavone glycoside, containing apigenin-6-C-Beta-D-galactose-8-C-Alpha-L-arabinoside and apigenin-6-C-Alpha-L-arabinoside-8-C-Beta-D-galactoside. The invention also relates to the extraction method of the chickweed flavone glycoside extraction, the drug compound containing the extractive and the purpose for preparing lipid lowering drug.

The invention relates to a windflower extract, preparation method and application thereof. The windflower extract comprises 2-10 wt% of oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-alpha-L- rhamnopyranosyl-(1-2)-alpha-L-arabinopyranoside, 10-40 wt% of hederagenin-3-O-alpha-L-rhamnopyranosyl-(1-2)[beta-D-glucopyranosyl-(1-4)]-alpha-L-arabinopyranoside, and 5-30 wt% of oleanolic acid-3-O-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-2)-alpha-L-arabinopyranoside, and the total saponin of the extract is larger than 65%. The content of active components in the windflower extract is improved, simultaneously the impurities in the extract are reduced, thereby the therapeutic effect of the windflower extract is increased.

The invention provides a medicinal preparation containing albizia flower total flavone and medical application. The albizia flower total flavone contains the following components in percentage by weight: 41-50 percent of quercitrin, 0.1-10 percent of quercetin, 0.1-5 percent of kaempferide, 0.1-2 percent of quercetin-3-0-alpha-L-arabinoside, and quercetin-3-0-alpha-L-arabinoside. The invention also discloses a method for preparing the albizia flower total flavone medicinal preparation with high dissolution rate, as well as application of the albizia flower total flavone and medical preparation thereof serving as basic active substances in preparing a medicine for treating depression.

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing oneor several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

The invention discloses a synchronous induced synthesis method of arabinosidase and xylanase. Trichoderma reesei is used as an enzyme-producing strain, and semen plantaginis husk, semen plantaginis polysaccharide or a mixture of the semen plantaginis husk and the semen plantaginis polysaccharide is used as a carbon source inducer to ferment in an enzyme-producing culture medium; the synchronous induced synthesis of the arabinosidase and the xylanase is realized. The enzyme liquid with high activity of arabinosidase and xylanase produced by the invention can meet the requirement of high-efficiency preparation of arabinoxylooligosaccharide by taking arabinoxylan as an enzyme hydrolysis object.