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Content measurement method of abrus herb capsules

A determination method, the technology of Jigucao, is applied in the field of content determination of Jigucao capsules, achieving the effect of perfect quality standard, strong specificity and good reproducibility

Active Publication Date: 2011-05-11
GUANGXI YULIN PHARM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the present invention, based on the research on the chemical composition and quality control of the specialty medicinal material Jigucao in Guangxi, the HPLC-UV method is used to measure the apigenin-6,8 in the monarch drug Jigucao in the preparation Jigucao capsule under the same chromatographic condition -Di-C-glucoside (apigenin-6, 8-di-C-β-D glucopyranoside, Vicenin, 1), apigenin-6-C-arabinose-8-C-glucoside (apigenin 6-C- α-L-arabinopyranosyl-8-C-β-D-glucopyranoside, Isoschaftoside, 2) apigenin-6-C-glucose-8-C-arabinoside (apigenin 6-C-β-D-glucopyranosyl-8-C - α-L-arabinopyranoside, Schaffoside, 3) content, at present, there is no relevant technology reported in the literature

Method used

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  • Content measurement method of abrus herb capsules
  • Content measurement method of abrus herb capsules
  • Content measurement method of abrus herb capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Determination according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 edition).

[0104] a) Preparation of the test solution Take the content of the chicken bone grass capsule, grind it finely, take about 0.5g, weigh it accurately, add 50% methanol 50ml, heat and reflux in a water bath for 2 hours, filter, and use 15ml 50% methanol for the residue Wash and filter several times, combine the filtrate and lotion and evaporate to dryness on a water bath, add water to dissolve the residue (2.0, 1.0, 1.0, 1.0ml), quantitatively add to the treated polyamide column (60-90 mesh , internal diameter 1.0cm, length 10cm, wet packing), let stand for 10 minutes, wash with 10ml of water, discard the water wash, then wash with 40ml of 60% methanol, collect the 60% methanol eluate, put it on a water bath and evaporate Dry, add 50% methanol to dissolve the residue in stages, quantitatively transfer it into a 10ml measuring bottle, add 50% methanol ...

Embodiment 2

[0112] Determination according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 edition).

[0113] a) Preparation of the test solution Take the content of the chicken bone grass capsule, grind it finely, take about 0.6g, weigh it accurately, add 50ml of 70% methanol, heat and reflux in a water bath for 3 hours, filter, and use 15ml of 70% methanol for the residue Wash and filter several times, combine the filtrate and lotion and evaporate to dryness on a water bath, add water to dissolve the residue (2.0, 1.0, 1.0, 1.0ml), quantitatively add to the treated polyamide column (60-90 mesh, Inner diameter 1.0cm, length 10cm, wet packing), let stand for 10 minutes, eluted with 15ml of water, discarded the water washing solution, then eluted with 30ml of 70% methanol, collected the 70% methanol eluate, and evaporated to dryness on a water bath , add 60% methanol to dissolve the residue in stages, quantitatively transfer it into a 10ml volumetric ...

Embodiment 3

[0121] Determination according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2005 edition).

[0122] a) Preparation of the test solution Take the content of the chicken bone grass capsule, grind it finely, take about 0.4g, weigh it accurately, add 50% methanol 50ml, heat and reflux in a water bath for 2 hours, filter, and use 20ml 30% methanol for the residue Wash and filter several times, combine the filtrate and lotion and evaporate to dryness on a water bath, add water to dissolve the residue (2.0, 1.0, 1.0, 1.0ml), quantitatively add to the treated polyamide column (60-90 mesh, Inner diameter 1.0cm, length 10cm, wet packing), let stand for 10 minutes, eluted with 8ml of water, discarded the water wash, then eluted with 40ml of 50% methanol, collected 50% methanol eluate, evaporated to dryness on a water bath , add 40% methanol to the residue to dissolve in stages, quantitatively transfer it into a 10ml measuring bottle, add 40% methanol ...

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Abstract

The invention discloses a content measurement method of abrus herb capsules. In the method, a high performance liquid chromatography-ultraviolet (HPLC-UV) method is used for measuring the contents of apigenin-6,8-di-C-glucoside, apigenin-6-C-arabinose-8-C-glucoside and apigenin-6-C-glucose-8-C-arabinoside in abrus mollis hance (monarch drug in a prescription) in the abrus herb capsules under the same chromatographic condition. The method comprises the following steps: preparing a test sample solution; preparing a control sample solution; and measuring by gradient elution. The method has the advantages of strong specificity and better accuracy, precision and reproducibility, is simple and easy to operate, and has important significance for further perfecting the quality standards of the abrus herb capsules.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a content determination method of Jigucao capsules. Background technique [0002] Jigucao Capsule is a famous and high-quality product of traditional Chinese medicine produced by Guangxi Yulin Pharmaceutical Co., Ltd. based on folk prescriptions and improved after years of trial production. In more than 20 years of production, sales and clinical use, it has been proved to have good clinical curative effect and enjoy a certain reputation in the market. At present, it has become a Chinese patent medicine for treating acute and chronic hepatitis and cholecystitis. It is a common medicine used by patients and ordinary people for clearing away heat and detoxification, clearing liver and protecting liver. This prescription is composed of herbal medicines such as Phyllostachys japonicus, gardenia, and capillary. It has the functions of soothing the liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/815G01N30/02A61P1/16
Inventor 汪豪叶志文欧彪蒙毅
Owner GUANGXI YULIN PHARM GRP CO LTD
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