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Preparation method of flavonoid glycosides in scutellaria baicalensis

A technology of flavonoid glycosides and baicalensis, applied in the field of separation of natural medicines, can solve the problems of low column efficiency, limitation of simultaneous separation of multiple compounds, high-throughput preparation of unfavorable compounds, etc., and achieves short cycle time, guaranteed reproducibility and stability. , good reproducibility

Inactive Publication Date: 2010-06-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the generally large particle size stationary phase, the column efficiency is low, which limits the simultaneous separation of multiple compounds, which is not conducive to the high-throughput preparation of compounds

Method used

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  • Preparation method of flavonoid glycosides in scutellaria baicalensis
  • Preparation method of flavonoid glycosides in scutellaria baicalensis
  • Preparation method of flavonoid glycosides in scutellaria baicalensis

Examples

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Embodiment 1

[0024] Embodiment 1: the preparation method of flavonoid glycosides in Scutellaria baicalensis

[0025] Crush the raw medicinal material of Scutellaria baicalensis, weigh 2 kg quantitatively, put it in a 20-liter extraction tank, add 20 liters of water to decoct for 1 hour, filter, and save the filtrate for later use, add 20 liters of water to the filter residue and decoct for 1 hour, filter, and the filtrate and the second Once combined, the filter residue was discarded. Concentrate the extract by rotary evaporation to 500 ml to obtain the water extract fraction of Scutellaria baicalensis. Add 710 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, concentrate the filtrate to 300 milliliters, add 1600 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours hour, filter, filter residue is discarded, the filtrate is concentr...

Embodiment 2

[0044] Crush the raw medicinal material of Scutellaria baicalensis, weigh 4 kg quantitatively, put it in a 100-liter extraction tank, add 32 liters of water to decoct for 2 hours, filter, and save the filtrate for later use, add 32 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate and the second Once combined, the filter residue was discarded. The extract was concentrated to 800 ml by rotary evaporation to obtain the water extract fraction of Scutellaria baicalensis. Add 1370 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, and concentrate the filtrate to 400 milliliters, add 2140 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours. hour, filter, filter residue is discarded, the filtrate is concentrated to no alcohol smell, and the concentrated solution is centrifuged through a hig...

Embodiment 3

[0048] Scutellaria baicalensis raw medicinal materials were pulverized, weighed 6 kg quantitatively, placed in a 100-liter extraction tank, added 36 liters of water and decocted for 3 hours, filtered, and the filtrate was stored for later use, and 36 liters of water were added to the filter residue and decocted for 3 hours, filtered, and the filtrate was mixed with the second Once combined, the filter residue was discarded. The extract was concentrated to 1200 ml by rotary evaporation to obtain the water extract fraction of Scutellaria baicalensis. Add 2050 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, concentrate the filtrate to 900 milliliters, add 4800 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours hour, filtered, and the filter residue was discarded, the filtrate was concentrated until there was no alcohol...

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Abstract

The invention provides a preparation method of flavonoid glycosides in scutellaria baicalensis. The method is suitable for quickly preparing four flavonoid glycosides comprising baicalin, wogonoside, chrysin-6-C-alpha-L-Arabic glucoside-8-C-beta-D-glucoside and oroxylinA-7-O-glucuronide. The preparation method comprises the following steps of: pulverizing scutellaria baicalensis and then adding water to extract; sequentially carrying out alcohol precipitation and high-speed centrifugation; after membrane separation, loading nonpolar macroporous absorption resin and eluting by alcohol / water; collecting eluent and concentrating and drying to obtain a scutellaria baicalensis macroporous resin component; and obtaining a scutellaria baicalensis effective component through parallel preparing liquid-phase chromatographic separation by using acetonitrile water as a flow phase. The preparation method has good selectivity and high purity of the obtained compound and greatly improves the separation and purification efficiency. The preparation process has high repeatability and good maneuverability, is easy to realize standardization and industrialization and has certain guidance effects on quickly obtaining flavones active constituents in the scutellaria baicalensis.

Description

technical field [0001] The present invention relates to the separation of natural medicines, in particular to the extraction and preparation method of flavonoid glycosides in Scutellaria baicalensis, including baicalin, wogonin, chrysin-6-C-α-L-arabinose-8-C-β-D - Preparation of glucosides and oreolin A-7-O-glucuronide. Background technique [0002] Scutellaria baicalensis Georgi, a traditional Chinese medicine, is the dried root of Scutellaria baicalensis Georgi. It has the functions of clearing away heat and dampness, purging fire and detoxifying, cooling blood and stopping bleeding, and removing heat and preventing miscarriage. It is mainly produced in Hebei, Shanxi, Inner Mongolia, Shandong and Henan. Scutellaria baicalensis mainly contains several major compounds such as flavonoids, terpenes, sterols and organic acids (Liu Xiong et al., Journal of Gansu University of Traditional Chinese Medicine, 24(2): 46-51). Among them, flavonoids represented by baicalin, wogonin, ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 梁鑫淼王艳萍丰加涛肖远胜薛兴亚章飞芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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