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Method for preparing triterpene compound with effects of preventing and treating obesity and application of triterpene compound

A triterpenoid compound and a technology for its use are applied in the field of preparation of a triterpenoid compound with the effect of preventing and treating obesity and the field of use thereof, which can solve the problems such as the lack of relevant research reports on the effect of weight loss, and achieve the effect of effectively treating obesity, preventing and treating obesity, inhibiting the effect of obesity. Effects of adipogenesis

Inactive Publication Date: 2017-09-26
CHANGSHA BROAD OCEAN BIO SCI & TECHN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active ingredients of Radix Acanthopanax have many biological activities, but there is no relevant research report on its weight loss effect

Method used

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  • Method for preparing triterpene compound with effects of preventing and treating obesity and application of triterpene compound
  • Method for preparing triterpene compound with effects of preventing and treating obesity and application of triterpene compound
  • Method for preparing triterpene compound with effects of preventing and treating obesity and application of triterpene compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Extraction: Grind 10 kg of dried Acanthopanax pratense (including roots, stems, and leaves), use 100 L of ethanol as a solvent, heat and reflux at 80° C. to extract 3 times, combine and recover the solvent under reduced pressure to obtain an extract;

[0029] (2) degreasing: step (1) medicinal extract carries out degreasing process with sherwood oil;

[0030] (3) Extraction: after step (2) degreasing, put the extract on D101 macroporous resin column, elute with 30%-95% ethanol gradient, collect 80%-95% ethanol part, recycle ethanol after merging, and dry to dry paste ; Mix the dry paste with silica gel, pass through silica gel column chromatography, and wash with a gradient of 42:1:0, 24:1:0, 14:1:0, 9:1:0.1 by volume ratio of chloroform:methanol:water Among them, the 9:1:0.1 portion was then eluted with a gradient of 8:1:0.1, 6:1:0.15 in the volume ratio of chloroform:methanol:water, and the 8:1:0.1 portion was collected. Sugar gel column Sephadex LH-20, eluted wi...

Embodiment 2

[0032] (1) Extraction: Grind 30 kg of dried fine column five, use 200 L of methanol as solvent, heat and reflux at 75° C. to extract 3 times, and after merging, recover the solvent under reduced pressure to obtain an extract;

[0033] (2) degreasing: step (1) medicinal extract carries out degreasing process with sherwood oil;

[0034] (3) Extraction: After step (2) is degreased, the extract is put on an AB-8 macroporous resin column, and is eluted with a gradient of 30%-100% ethanol, and the 60%-95% ethanol part is collected, and the ethanol is recovered after merging, and dried to Dry paste; mix the dry paste with silica gel, pass through silica gel column chromatography, use 30:1:0, 20:1:0, 12:1:0, 8:1:0.1 by volume ratio of chloroform:methanol:water Gradient elution, wherein, the 8:1:0.1 part was then eluted with a volume ratio of 10:1:0.1, 5:1:0.15 in chloroform:methanol:water, and the 10:1:0.1 part was collected, the solvent was removed, and the Sephadex LH-20, eluted wi...

Embodiment 3

[0036] (1) Extraction: Add 25kg of dried Evodia rutaecarpa, use 180L methanol as a solvent, heat and reflux at 78°C to extract twice, combine and recover the solvent under reduced pressure to obtain an extract;

[0037] (2) degreasing: step (1) medicinal extract carries out degreasing process with sherwood oil;

[0038](3) Extraction: step (2) after degreasing, put the extract on a macroporous resin column, elute with 40%-100% ethanol gradient, collect 80%-95% ethanol part, recycle ethanol after merging, and dry to dry paste; Mix the dry paste with silica gel, pass through silica gel column chromatography, and use the volume ratio of chloroform:methanol:water to be 36:1:0, 22:1:0, 13:1:0, 9:1:0.1 gradient elution , where the 9:1:0.1 fraction was eluted with a volume ratio of 8:1:0.1, 4:1:0.15 in chloroform:methanol:water, and the 8:1:0.1 fraction was collected, removed from the solvent, and washed with dextran Gel column Sephadex LH-20, eluted with 90% methanol to obtain the ...

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Abstract

The invention discloses a method for preparing a triterpene compound oleanolic acid 3-O-[beta-D-glucopyranose-(1->3)]-beta-D-galactopyranose-(1->2)-alpha-L-pyrane glucosidase arabinofuranoside (Glycoside St-E2) and application of the triterpene compound. The triterpene compound is separated from acanthopanax plants. The method and the application have the advantages that the method includes simple steps and is high in extraction efficiency and product purity; as discovered in mouse preadipocyte culture tests, adipogenesis can be obviously inhibited by the triterpene compound by the aid of AMPK-PPAR gamma-C / EBP alpha mechanisms, and accordingly the triterpene compound is an effective medicine for preventing and improving obesity.

Description

technical field [0001] The invention relates to the technical field of natural medicines, in particular to a triterpene compound oleanolic acid 3-O-[β-D-glucopyranose-(1→3)] isolated from Radix Acanthopanax and having the effect of preventing and treating obesity - Preparation method and application of β-D-galactopyranose-(1→2)-α-L-arabinopyranoside (Glycoside St-E2), especially in the preparation of food for preventing obesity and the prevention and treatment of obesity Functional medicines and health care products. Background technique [0002] In recent decades, with the continuous improvement of living standards and changes in lifestyles, the incidence of obesity has been increasing year by year, and the trend of younger age is obvious. The rapid rise in obesity poses serious social and economic challenges. In addition, obesity is also the cause of various adult diseases such as cancer, diabetes, high blood pressure, hyperlipidemia, and metabolic syndrome, so treating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61P3/04
CPCC07J63/008
Inventor 刘向前李芝邹亲朋戴玲洪承宪韩耀韩
Owner CHANGSHA BROAD OCEAN BIO SCI & TECHN CO LTD
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