Separation and purification method and application of malvidin-3-O-glucosidase arabinofuranoside

An arabinoside, separation and purification technology, which is applied in the field of separation and purification of natural products, can solve the problem that there is no application of malvain-3-O-arabinoside monomer, and no separation and preparation of malvain-3-O-arabinoside is found. problems such as glycoside monomers, to achieve the effect of large sample processing capacity and good repeatability

Active Publication Date: 2018-11-23
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, there is no research and report on the isolation and preparation of malvatin-3-O-arabinoside monomers from blueberries, and no such malvatin-3-O-arabinoside monomers are used in the preparation of α-glucosidase. Applications in Inhibitors

Method used

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  • Separation and purification method and application of malvidin-3-O-glucosidase arabinofuranoside
  • Separation and purification method and application of malvidin-3-O-glucosidase arabinofuranoside
  • Separation and purification method and application of malvidin-3-O-glucosidase arabinofuranoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Wash 1 kg of blueberries, add 70% (volume ratio of ethanol to water: 70:30) ethanol aqueous solution containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid 1:8 (w / v, g / mL) (The volume ratio of ethanol to water is 70:30), and ultrasonically extract at 40°C for 60 minutes in the dark, then vacuum filter, and the filter residue is repeatedly extracted once, the filtrates are combined, and the ethanol is removed by vacuum rotary evaporation at 45°C to obtain blueberry flower color Glycoside crude extract.

[0068] The blueberry anthocyanin crude extract was added to the same volume of ethyl acetate for extraction, extracted 4 times, the water phase was collected, and then concentrated by rotary evaporation in a vacuum at 45°C to remove the residual ethyl acetate to obtain anthocyanin extract.

[0069] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol...

Embodiment 2

[0074] Wash 5kg of blueberry anthocyanins, add acidified 60% (v / v) ethanol solution according to the ratio of material to liquid (1:8 (w / v), and extract by ultrasound at 40°C for 70 minutes in the dark, then vacuum filter, and the filter residue The extraction was repeated once, the filtrates were combined, and the ethanol was removed by vacuum rotary evaporation at 45° C. to obtain a crude blueberry anthocyanin extract.

[0075] The blueberry anthocyanin crude extract was added to the same volume of ethyl acetate for extraction, extracted 4 times, the water phase was collected, and then concentrated by rotary evaporation in a vacuum at 45°C to remove the residual ethyl acetate to obtain anthocyanin extract.

[0076]Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized w...

Embodiment 3

[0080] Wash 10kg of blueberry anthocyanins, add acidified 60% (v / v) ethanol solution according to the ratio of solid to liquid 1:8 (w / v), and extract by ultrasound at 40°C for 90 minutes in the dark, then vacuum filter, and the filter residue The extraction was repeated once, the filtrates were combined, and the ethanol was removed by vacuum rotary evaporation at 45° C. to obtain a crude blueberry anthocyanin extract.

[0081] The blueberry anthocyanin crude extract was added to the same volume of ethyl acetate for extraction, extracted 4 times, the water phase was collected, and then concentrated by rotary evaporation in a vacuum at 45°C to remove the residual ethyl acetate to obtain anthocyanin extract.

[0082] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized wat...

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Abstract

The invention discloses a separation and purification method of malvidin-3-O-glucosidase arabinofuranoside and application of the malvidin-3-O-glucosidase arabinofuranoside in preparing an alpha-glucosidase inhibitor. The preparation and purification method comprises the steps of coarse extraction, macroporous resin adsorption, high-speed countercurrent chromatographic separation and solid-phase extraction and purification. The separation and purification method is characterized by combining high-speed countercurrent chromatography with a solid-phase extraction technology, and then optimizingtechnological parameters, thus separating a complicated blueberry raw material of an anthocyanin composition, thus preparing a high-purity malvidin-3-O-glucosidase arabinofuranoside monomer. An activity experiment discovers that the malvidin-3-O-glucosidase arabinofuranoside monomer is capable of remarkably inhibiting the activity of alpha-glucosidase and can be used for preparing the alpha-glucosidase inhibitor.

Description

technical field [0001] The invention relates to the field of separation and purification of natural products, in particular to a method for separation and purification of malvatin-3-O-arabinoside and its application in the preparation of alpha-glucosidase inhibitors. Background technique [0002] Diabetes is one of the common chronic diseases worldwide. Diabetic patients are usually accompanied by symptoms of hyperglycemia. Long-term hyperglycemia can cause damage to tissues and organs such as nerves, heart, blood vessels and kidneys, and cause a variety of acute and chronic complications. . According to the statistics of the World Health Organization (WHO), there were 422 million diabetic patients in the world in 2014, of which type 2 diabetes is the most common. In recent years, with the changes in people's eating habits and lifestyles and the acceleration of the aging process, the prevalence of diabetes in my country has risen rapidly. By the end of 2016, the number of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08A61P3/10
CPCA61P3/10C07H1/08C07H17/065
Inventor 陈卫徐阳谢佳宏谢亮华
Owner ZHEJIANG UNIV
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