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Chickweed flavonoside extract, its preparation method and medicine made therefrom application

A technology for extracts and flavonoid glycosides, applied in the field of flavonoid glycoside extracts, can solve the problems of insufficient pharmacological research, no blood lipid-lowering active components, and no research reports on the chemical components and their activities of chickweed systems.

Inactive Publication Date: 2008-01-16
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its pharmacological research is still very insufficient, mainly antipyretic, anti-inflammatory effects (Wang Yinghua et al., comparative study on the chemical constituents of introduced species and wild ginkgo, Chinese Journal of Pharmaceutical Sciences, 1991.26 (5): 266) and antioxidant effects (Budzianowski J etc., Studies on the antioxidant activity of some C-glycosylflavones, J.Pharmacol Pharm., 1991, 435): 395)
[0006] It can be seen from the existing literature reports that there is no research report on the chemical constituents of chickweed system and their activities, especially no reports on its blood lipid-lowering active ingredients

Method used

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  • Chickweed flavonoside extract, its preparation method and medicine made therefrom application
  • Chickweed flavonoside extract, its preparation method and medicine made therefrom application
  • Chickweed flavonoside extract, its preparation method and medicine made therefrom application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1: Preparation of chickweed flavonoid glycoside extract (1)

[0076] Dry the whole chickweed and crush it. 6 kg of the crushed chickweed was added to 8 times the amount of 70v / v% ethanol for reflux extraction, filtered to obtain an ethanol extract. Extract again in the same way, combine the two extracts, concentrate to remove ethanol, and obtain the extract. The extract is dissolved in water, centrifuged (2500 rev / min, 25min) to remove insoluble matter, and the supernatant is subjected to HP-20 macroporous adsorption resin column chromatography, the ratio of resin column bed volume (ml) to sample amount (grams) It is 10:1. After elution with water, and then elution with 35v / v% ethanol, the elution part was concentrated to remove the solvent and then freeze-dried to obtain 80 grams of brown yellow powder, which was the extract of chickweed flavonoid glycosides, which had the aforementioned qualitative reaction characteristics, indicating that Chickweed flav...

Embodiment 2

[0078] Embodiment 2: Preparation of chickweed flavonoid glycoside extract (2)

[0079] Dry the whole chickweed, crush it, weigh 12 kg, add 8 times the amount of 60v / v% ethanol for reflux extraction, and filter to obtain the ethanol extract. Extract again in the same way, combine the two extracts, concentrate to remove ethanol, and obtain the extract. The extract is dissolved in water, centrifuged (2500 rev / min, 25min) to remove insoluble matter, and the supernatant is subjected to HP-20 macroporous adsorption resin column chromatography, the ratio of resin column bed volume (ml) to sample amount (grams) It is 10:1. After water elution (with 7 column bed volumes of water), then elution with 30v / v% ethanol, the elution site was concentrated to remove the solvent and then freeze-dried to obtain 152 grams of brown-yellow powder, which was the chickweed flavonoid glycoside extract. It has the aforementioned qualitative response characteristics.

[0080] The contents of the two m...

Embodiment 3

[0081] Example 3: Biological activity experiment of chickweed flavonoid glycosides extract

[0082] Observation indicators: Total cholesterol and triglyceride levels in rats with hyperlipidemia induced by high-fat diet.

[0083] Experimental Materials

[0084] Animals: adult SD rats, about 200 g, male, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0085] Reagents: sodium cholate (Beijing Shuangxuan Microbial Medium Products Factory, batch number 20041102), propylthiouracil (Suzhou Hengyi Pharmaceutical Raw Materials Co., Ltd., batch number 20050220); cholesterol: purchased from Beijing Chemical Reagent Company; Lipingzhi capsule (Fenofibrate, produced by France Liboforni Pharmaceutical Company, batch number 65719). Total cholesterol (CHO) kit, triglyceride (TG) kit, high-density lipoprotein cholesterol (HDL-C) kit, low-density lipoprotein cholesterol (LDL-C) kit are Beijing Beihua Kangtai clinical reagents Co., Ltd. products; all non-water-so...

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Abstract

The invention relates to extractive of chickweed flavone glycoside, containing apigenin-6-C-Beta-D-galactose-8-C-Alpha-L-arabinoside and apigenin-6-C-Alpha-L-arabinoside-8-C-Beta-D-galactoside. The invention also relates to the extraction method of the chickweed flavone glycoside extraction, the drug compound containing the extractive and the purpose for preparing lipid lowering drug.

Description

technical field [0001] The invention relates to a flavonoid glycoside extract of the traditional Chinese medicine chickweed, its extraction method, a pharmaceutical composition containing the extract and its use for preparing blood lipid-lowering drugs. technical background [0002] Chickweed [Stellaria media (L.) Cyr.] is a plant of the genus Stellaria (Stellaria L.) in the family Caryophyllaceae. It has the effects of clearing away heat and detoxifying, promoting blood circulation, relieving pain, and reducing swelling. , Intestinal carbuncle, furuncle, bruises, postpartum stasis and abdominal pain. [0003] Chickweed is the whole herb of Stellaria L. plant Chickweed Stellaria media (L.) Cyr. in Caryophyllaceae Caryophyllaceae. Alias ​​蔜 ("Erya"), 蘩蒌 ("Bie Lu"), Zicao ("Qian Jin Shizhi"), goose sausage, goose sausage ("Compendium"), five-clawed dragon ("Hunan Medicine Zhi "), dog flea dish ("Guangxi Medicine Plant List"), goose wonton (Su Yi "Handbook of Chinese Herbal M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K36/36A61P3/06
Inventor 乔善义董琦黄元
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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