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Method for producing multiple kinds of monosaccharide through bamboo processing residues

A technology for processing residues and monosaccharides, applied in the field of monosaccharide preparation, can solve the problems of aggravation, large loss of cellulose and hemicellulose, unsuitable lignocellulose pretreatment methods, etc., achieve good practicability and improve accessibility degree of effect

Inactive Publication Date: 2015-03-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of conventional kraft cooking of plant fiber raw materials to produce pulp, the amount of alkali is generally more than 25%, and the plant fiber raw materials are cooked under this condition. While most of the lignin is removed, part of the cellulose and hemicellulose The peeling reaction and alkaline hydrolysis are also intensified, and the loss of cellulose and hemicellulose in the cooking process is large, so the conventional kraft cooking method for pulp production is not suitable as a pretreatment method for lignocellulose biorefinery

Method used

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  • Method for producing multiple kinds of monosaccharide through bamboo processing residues
  • Method for producing multiple kinds of monosaccharide through bamboo processing residues
  • Method for producing multiple kinds of monosaccharide through bamboo processing residues

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Put 100g of absolutely dried bamboo chips into a 1L stainless steel tubular digester, add NaOH and NaOH at a solid-to-liquid ratio of 1:5 2 S, the degree of sulfide is 20%, the amount of alkali is 2-10% (referring to NaOH+Na 2 S content, as Na 2 O meter) cooking liquor. After pre-soaking at 80°C for 20min, the temperature was raised to 160°C at a rate of 2°C / min and kept for 1h. Immediately after the reaction, take out the digester and immerse it in cold water, add distilled water to the cooked product to adjust the solid content to 5%, refine the pulp in a disc refiner at 3000 rpm for 30 minutes, separate the solid from the liquid, and wash the solid residue with distilled water to neutral. Put the solid slag into a sealed bag, balance the moisture in a refrigerator at 4°C for 2 days, measure the content of cellulose, xylan and lignin in the material, and calculate the recovery rate of cellulose, xylan and lignin removal rate, The results obtained are shown in Tabl...

Embodiment 2

[0036] Take by weighing respectively the solid slag 7.59g (2.5g of absolute dry weight) that alkali consumption is 10% kraft method cooking pretreatment obtains in embodiment 1 in 10 250mL conical flasks, in each conical flask, add 1mol / L citric acid buffer 2.5mL, add the cellulase that enzyme dosage is 0, 5, 10, 15, 20, 25, 30, 35, 40, 45FPU / g cellulose in each triangular flask respectively, in each triangular flask Add an appropriate amount of distilled water to the bottle to make the total water volume in the enzymatic hydrolysis system 50mL, mix the reaction system thoroughly with a glass rod, cover the cap, and perform enzymatic hydrolysis in a constant temperature shaker at 150 rpm and 50°C for 48 hours. After the hydrolysis, the hydrolyzate was centrifuged at 4000 rpm for 10 min, and the supernatant was taken to measure the concentration of glucose, xylose and arabinose, and the enzymatic hydrolysis yield was calculated. Among them, cellulose enzymatic hydrolysis yield ...

Embodiment 3

[0039] Take by weighing respectively the solid slag 7.59g (2.5g of absolute dry weight) obtained by the kraft method cooking pretreatment that alkali consumption is 10% in embodiment 1 in 11 250mL conical flasks, add 1mol / L in each conical flask Citrate buffer 2.5mL, add the β-glucosidase that consumption is 0,3,6,9,12,15,18,21,24,27,30U / g cellulose in each Erlenmeyer flask respectively, in Add an appropriate amount of distilled water to each flask to make the total volume of water in the enzymolysis system 50mL, mix the reaction system well with a glass rod, cover the lid, and enzymolyze in a constant temperature shaker at 150 rpm and 50°C for 48 hours . After the hydrolysis, the hydrolyzate was centrifuged at 4000 rpm for 10 min, and the supernatant was taken to measure the concentration of glucose, xylose and arabinose, and the enzymatic hydrolysis yield was calculated.

[0040] The effects of different amounts of β-glucosidase on the enzymatic hydrolysis yield of cellulos...

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Abstract

The invention discloses a method for producing multiple kinds of monosaccharide through bamboo processing residues. According the method, the bamboo processing residues are pretreated through cooking in a sulfate process with the alkali dosage being 2% to 10%, defibrination, washing, and saccharification achieved through a special enzyme preparation are conducted on the pretreated materials, and then glucose, xylose and arabinose are efficiently produced. The special enzyme preparation comprises 5-45 FPU / g cellulose, 3-30 U / g beta-cellulose glucosidase, 15-150 U / g cellulose xylanase and 0.3-2.1 U / g cellulose arabinosidase. According to the method, the bamboo processing residues are pretreated through cooking in the sulfate process with low alkali dosage, beta-aryl ether bonds in lignin are destroyed, and part of the lignin is removed; pretreatment of fiber defibering and devillicate brooming are conducted on the pretreated materials which are cooked in the sulfate process with low alkali dosage in a disk mill, effective separation of the cellulose, hemicellulose and the lignin is further achieved, and the accessibility of the cellulose and the hemicellulose to enzymes is improved; then, under the saccharification action of the special enzyme preparation, the materials are efficiently hydrolyzed into the glucose, the xylose and the arabinose.

Description

technical field [0001] The invention relates to the technical field of monosaccharide preparation, in particular to a method for producing various monosaccharides from bamboo processing residues. Background technique [0002] The area of ​​China's bamboo forest has reached 4.8 million hectares, with an annual output of 3 billion pieces of bamboo. In the south, bamboo processing industries for bamboo flooring, bamboo furniture and bamboo-wood composite materials have been formed. Bamboo processing industry produces as much as 46 million tons of bamboo processing residues (bamboo chips) every year. Except for some of these wastes being used, a large amount of wastes are discarded, which not only pollutes the environment, but also causes waste of biomass resources. [0003] Cellulose and hemicellulose in lignocellulose can be degraded into monosaccharides under the catalysis of enzymes, and monosaccharides can be fermented by microorganisms to produce bioenergy such as ethanol ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 勇强黄曹兴李鑫赖晨欢徐勇欧阳嘉余世袁
Owner NANJING FORESTRY UNIV
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