Synchronous induced synthesis method of arabinosidase and xylanase

An arabinosidase and xylanase technology, which is applied in the field of microbial culture in biochemistry, can solve the problem that key hydrolases cannot be efficiently and synergistically prepared, and achieve the effects of efficient preparation and improved hydrolysis efficiency.

Pending Publication Date: 2022-07-26
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: Aiming at the bottleneck problem that the key hydrolase (arabinosidase and xylanase) in the process of preparing arabinoxylan xylo-oligosacchari

Method used

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  • Synchronous induced synthesis method of arabinosidase and xylanase
  • Synchronous induced synthesis method of arabinosidase and xylanase
  • Synchronous induced synthesis method of arabinosidase and xylanase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of psyllium polysaccharide with different molecular weights comprises the following steps:

[0029] (1) The psyllium husk was mechanically pulverized, sieved through a 100-mesh sieve, and the solid-liquid ratio (g / mL) was 1:20, extracted for 12 hours at 170 rpm in a constant temperature shaker at 50°C, and then centrifuged at 10,000 rpm. Collect the supernatant for 10 min, and the supernatant is the psyllium polysaccharide mixed solution;

[0030] (2) Take the psyllium polysaccharide mixed solution obtained in step (1), add absolute ethanol under constant stirring, control the ethanol concentration (volume concentration) in the system to be 15%, and separate the mixed solution through solid-liquid separation to obtain a supernatant 1 and the precipitation, the precipitation is washed 3 times in the ethanolic solution of 15% (volume concentration), and freeze-drying is the psyllium polysaccharide component 1, and the average of the psyllium polysacch...

Embodiment 2

[0036] The fermentation enzyme production method using psyllium husk and psyllium polysaccharides with different molecular weights as inducing carbon sources mainly includes the following steps:

[0037] (1) Weigh 20.00g of psyllium husk and psyllium polysaccharide of different molecular weights (prepared in Example 1), 1.00g of glucose, 20.00g of potassium dihydrogen phosphate, 3.00g of urea, 1.50g of anhydrous magnesium sulfate, and sulfuric acid Ammonium 14.00g, Calcium Chloride 4.00g, FeSO 4 ·7H 2 O 5.00mg, MnSO 4 ·7H 2 O 1.60mg, ZnSO 4 ·7H 2 O 1.40mg, CoCl 2 After fully dissolving 2.00 mg in distilled water, add 50 mL of 1 mol / L citric acid buffer solution, dilute the volume to 1000 mL with distilled water, divide the enzyme production medium into packages and place it at 121 °C for sterilization for 15 min.

[0038] (2) The Trichoderma reesei spore suspension (the number of spores is 2.0×10 7 cfu / mL) according to 10% of the inoculum and mixed evenly with the enzy...

Embodiment 3

[0044] The fermentation enzyme production method for inducing carbon source after the psyllium husk and psyllium polysaccharides of different molecular weights are mixed, mainly includes the following steps:

[0045] (1) The psyllium husk is fully pulverized in a mechanical pulverizer with component I, component П, component III and component IV in the embodiment (1) respectively, in a mass ratio of 1:1, They are denoted as Mixed I, Mixed П, Mixed III and Mixed IV.

[0046] (2) take by weighing respectively mixed I, mixed П, mixed III and mixed IV (prepared in step (1)) 20.00g, glucose 1.00g, potassium dihydrogen phosphate 20.00g, urea 3.00g, anhydrous magnesium sulfate 1.50g, Ammonium sulfate 14.00g, calcium chloride 4.00g, FeSO 4 ·7H 2 O 5.00mg, MnSO 4 ·7H 2 O 1.60mg, ZnSO 4 ·7H 2 O 1.40mg, CoCl 2 After fully dissolving 2.00 mg in distilled water, add 50 mL of 1 mol / L citric acid buffer solution, dilute the volume to 1000 mL with distilled water, divide the enzyme pr...

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Abstract

The invention discloses a synchronous induced synthesis method of arabinosidase and xylanase. Trichoderma reesei is used as an enzyme-producing strain, and semen plantaginis husk, semen plantaginis polysaccharide or a mixture of the semen plantaginis husk and the semen plantaginis polysaccharide is used as a carbon source inducer to ferment in an enzyme-producing culture medium; the synchronous induced synthesis of the arabinosidase and the xylanase is realized. The enzyme liquid with high activity of arabinosidase and xylanase produced by the invention can meet the requirement of high-efficiency preparation of arabinoxylooligosaccharide by taking arabinoxylan as an enzyme hydrolysis object.

Description

technical field [0001] The invention belongs to the technical field of microbial culture in biochemistry, and particularly relates to a method for the simultaneous induction and synthesis of arabinosidase and xylanase. Background technique [0002] Hemicellulose is the second most abundant renewable agricultural and forestry resource after cellulose, and arabinoxylan, an important member of the hemicellulose family, is mainly composed of xylose monomers polymerized by β-1,4-glycosidic bonds The resulting xylan backbone and α-1,2 / α-1,3-arabinosidic bonds are randomly substituted to form arabinose branches. Based on the molecular structure analysis of arabinoxylan, its hydrolase system mainly includes: xylanase, xylosidase, arabinosidase, etc., among which xylanase and arabinosidase are extremely important as key hydrolases. Compared with homogeneous xylan, arabinoxylan heteroheteropolysaccharides are affected by the steric hindrance of the branched structure, and the difficu...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N1/14C12P19/04C12P19/14C12R1/885
CPCC12N9/2402C12N9/248C12Y302/01088C12N1/14C12P19/04C12P19/14
Inventor 杨磊许伟颜子怡邵荣施晓龙帅晨宇吴梦霖
Owner YANCHENG INST OF TECH
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