Cultivation method for stevia rebaudiana tetraploid
A cultivation method and technology of stevia, which is applied in the field of stevia tetraploid cultivation, can solve the problems of low productivity of stevia and the inability of conventional growth to meet market demand, and achieve the effects of environmental friendliness, increased yield and broad application prospects
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Embodiment 1
[0025] The present embodiment relates to a method for cultivating stevia tetraploids, the method for cultivating comprises the following steps:
[0026] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;
[0027] (2) Seed treatment: Soak stevia seeds in detergent for 15 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 15 seconds, and finally wash them with sterile water for 3 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 25~30°C, culture in shading for 10 days, switch to light culture for 28 days for sterile culture, so that the germination of the seed embryo becomes a green seed embryo protocorm;
[0028] (...
Embodiment 2
[0032] The present invention relates to a kind of cultivation method of stevia tetraploid, and described cultivation method comprises the following steps:
[0033] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;
[0034] (2) Seed treatment: Soak stevia seeds in detergent for 15 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 20 seconds, and finally wash them with sterile water for 5 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 25°C, culture in shading for 7 days, then switch to light culture for 30 days for sterile culture, so that the seed embryos germinate into green seed embryo protocorms;
[0035] (...
Embodiment 3
[0039] The present invention relates to a kind of cultivation method of stevia tetraploid, and described cultivation method comprises the following steps:
[0040] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;
[0041](2) Seed treatment: Soak stevia seeds in detergent for 20 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 10 seconds, and finally wash them with sterile water for 5 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 30°C, culture in shading for 10 days, then switch to light culture for 25 days for sterile culture, so that the seed embryos germinate into green seed embryo protocorms;
[0042] (...
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