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Cuttage method of stevia rebaudiana rootless tissue culture seedling

A technology for stevia and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of restricting the expansion, promotion and application of tissue culture technology, low cutting survival rate of tissue culture seedlings, low cutting survival rate, etc. Reliable and feasible, fast effect of tissue culture propagation

Inactive Publication Date: 2011-01-19
崔广荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The disadvantages of the prior art are: the growth of test-tube seedlings is limited by environmental conditions, the seedlings are very delicate, the survival rate of cuttings is extremely low, and even rooted tissue culture seedlings are difficult to survive when transplanted directly.
The shortcoming of prior art is the inevitable result that brings because of test-tube plantlet growth environment, is a problem that can't be bypassed, is also a technical problem that is difficult to solve
The low survival rate of cuttings of tissue culture seedlings limits the expansion and application of tissue culture technology in Stevia rebaudiana

Method used

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  • Cuttage method of stevia rebaudiana rootless tissue culture seedling
  • Cuttage method of stevia rebaudiana rootless tissue culture seedling

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Embodiment 1

[0042]A method for cutting stevia rootless tissue culture seedlings, the steps are as follows:

[0043] Take out the proliferating seedlings in the tissue culture bottle, cut off the cutting seedlings with a length of 3 cm, remove the two leaves at the base, and keep the leaf nodes; keep the stevia seedlings moist during the cutting process to prevent drying and wilting.

[0044] Cutting: Take a foamed plastic seedling tray, fill the hole with fine sand, punch holes, and insert seedlings; ensure that a leaf node is buried in the sand and compact the fine sand; water; install a bracket on the seedling tray and cover it with plastic film .

[0045] Seedling tray management: the seedling trays are placed directly in the open air in spring; the seedling trays are covered with sunshade nets when the temperature is high in summer, and the sunshade nets are removed on cloudy summer days; the shading rate of the sunshade nets is 80%.

[0046] Seedling hardening: 10-15 days after plan...

Embodiment 2

[0048] A method for cutting stevia rootless tissue culture seedlings, the steps are as follows:

[0049] Take out the proliferating seedlings in the tissue culture bottle, cut off the cutting seedlings with a length of 4 cm, remove the two leaves at the base, and keep the leaf nodes; keep the stevia seedlings moist during the cutting process to prevent drying and wilting.

[0050] Cutting: Take a foamed plastic seedling tray, fill the hole with fine sand, punch holes, and insert seedlings; ensure that a leaf node is buried in the sand and compact the fine sand; water; install a bracket on the seedling tray and cover it with plastic film ;

[0051] (3) Seedling tray management: Put the seedling trays directly in the open air in spring; cover the seedling trays with sunshade nets when the temperature is high in summer, and remove the sunshade nets in cloudy summer days; the shading rate of the sunshade nets is 80%.

[0052] (4) Seedling hardening: 10-15 days after planting, rel...

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Abstract

The invention belongs to the technology of plant tissue culture and particularly relates to a cuttage method of stevia rebaudiana rootless tissue culture seedling. The existing stevia rebaudiana tissue culture rapid propagation technology has the following defects: the growth of the test-tube seedling is limited by the environmental conditions, the seedling is very slim and fragile, and the survival rate of cuttage is extremely low. The method of the invention comprises the following steps: a cuttage seedling is cut to 3-4cm, two basal leaves are removed, the leaf nodes are retained; a float tray is used, fine sand is filled in a planting hole, a hole is digged, the seedling is inserted; a support is arranged, a plastic film is covered; the float tray is directly placed in the open air in spring. When the temperature is high in summer, the float tray is covered by a sunshading net; ventilation is performed for hardening-seedling 10-15 days after planting; the plastic film is removed after 2-3 days; the sunshading net is removed after 1-2 days; liquid fertilizer is poured; and the strong seedling with numerous roots is cultured 20-25 days after cuttage. The invention has the following advantages: the production period is shortened, the consumed materials are reduced, the use of the device is saved, the utilization efficiency of the tissue culture room is increased, the energy is saved, the technical effect is good, and the transplanting survival rate is up to 99%.

Description

technical field [0001] The invention belongs to plant tissue culture technology, in particular to a cutting method for rootless tissue culture seedlings of Stevia rebaudiana. Background technique [0002] At present, there are more than 20 kinds of sweeteners used in the world, which can be divided into two categories: natural and synthetic according to their sources. Natural products include sucrose, fructose, honey, glucose, etc., all of which contain extremely high calories, which can easily lead to elevated blood sugar and dental caries. Diabetics, cardiovascular patients, and obese patients should not eat them. Chemical synthesis products include aspartame and saccharin, which are harmful to the human body. [0003] Stevia sugar extracted from Stevia rebaudiana is a new type of natural sweetener. A large number of drug experiments have proved that stevioside has no toxic side effects and is safe to eat. Regular consumption can prevent hypertension, diabetes, obesity, ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01H4/00
Inventor 崔广荣林平张从宇胡能兵刘宗巧张子学邹海明
Owner 崔广荣
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