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Codonopsis pilosula homogeneous polysaccharide with anti-gastric ulcer, preparation method and application

A technology for gastric ulcer and polysaccharide, which is applied in the field of preparation of active ingredients of traditional Chinese medicine, can solve the problem of no relevant reports on pharmacological activity and its mechanism research, etc.

Active Publication Date: 2018-11-13
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of the pharmacological studies reported in the literature are aimed at the total polysaccharides of Codonopsis pilosula, and there are almost no relevant reports on the pharmacological activity and mechanism of Codonopsis pilosula polysaccharides with uniform composition and clear structure.

Method used

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  • Codonopsis pilosula homogeneous polysaccharide with anti-gastric ulcer, preparation method and application
  • Codonopsis pilosula homogeneous polysaccharide with anti-gastric ulcer, preparation method and application
  • Codonopsis pilosula homogeneous polysaccharide with anti-gastric ulcer, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:

[0043] Take 100g of total polysaccharides of Codonopsis pilosula, add 2000mL of distilled water, and dissolve at 80°C for 1 hour, stirring continuously during this period. Stand at 4°C for 12 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 90% ethanol, allowed to stand at 4°C for 12 hours, and then centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 3000mL distilled water at 80°C for 1 hour, stir continuously during this period, let it stand at 4°C for 12 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure was 1.5 atmospheres, and the filtrate that finally passed through the 5000 molecular weight membrane was concentrated and dried to obtain 36.45 g of CPS-A.

Embodiment 2

[0045] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:

[0046]Take 100g of Codonopsis total polysaccharides, add 3000mL of distilled water, and dissolve at 85°C for 1.5 hours, stirring continuously during this period. Stand at 4°C for 24 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 95% ethanol, left at 4°C for 24 hours, and centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 4000mL distilled water at 85°C for 1.5 hours, stir continuously during this period, let stand at 4°C for 24 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure is 2.0 atmospheric pressure, the filtrate that finally passes through the 5000 molecular weight membrane is concentrated and dried to obtain 40.30 g of CPS-A.

Embodiment 3

[0048] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:

[0049] Take 100g of Codonopsis total polysaccharides, add 2500mL of distilled water, and dissolve at 85°C for 1 hour, stirring continuously during this period. Stand at 4°C for 24 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 95% ethanol, left at 4°C for 24 hours, and centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 3500mL distilled water at 85°C for 1 hour, stir continuously during this period, let stand at 4°C for 24 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure was 2.5 atmospheres, and the filtrate that finally passed through the 5000 molecular weight membrane was concentrated and dried to obtain 41.50 g of CPS-A.

[0050] The structural identification of Cod...

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Abstract

The invention discloses a radix codonopsis homogeneous polysaccharide with an anti-gastric ulcer effect. The polysaccharide has a molecular weight of 3000, and is a linear homogeneous polysaccharide formed by beta-D-fructofuranose residues through beta-(2->1)-glucosidic bonds. The separation and extraction method comprises the following steps: (1) extracting total radix codonopsis polysaccharides; and (2) performing ultrafiltration separation on the codonopsis polysaccharides. The invention further discloses an application of the radix codonopsis homogeneous polysaccharide in medicines for treating gastric ulcer.

Description

technical field [0001] The invention relates to a preparation method of active components of traditional Chinese medicine, in particular to a method for separating and purifying Codonopsis codonopsis homogeneous polysaccharide with anti-gastric ulcer effect and its anti-gastric ulcer effect. Background technique [0002] Codonopsis (Radix Codonopsis) is a plant belonging to the Codonopsis family Campanulaceae. It has the effects of invigorating the middle and nourishing qi, invigorating the spleen and lungs. It is clinically used for spleen and lung weakness, shortness of breath, palpitations, lack of food and loose stools, cough due to deficiency, and cough due to internal heat. Codonopsis pilosula mainly contains polysaccharides, saponins, triterpenes, alkaloids and other components, among which polysaccharides have the largest content and are one of the important components of Codonopsis pilosula. Modern pharmacological studies have shown that Codonopsis polysaccharides h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/04
CPCA61K31/715A61K36/344C08B37/0003C08B37/0051
Inventor 高建平李建宽杨丰榕王涛葛睿刘恩荔
Owner SHANXI MEDICAL UNIV
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