Codonopsis pilosula homogeneous polysaccharide with anti-gastric ulcer, preparation method and application
A technology for gastric ulcer and polysaccharide, which is applied in the field of preparation of active ingredients of traditional Chinese medicine, can solve the problem of no relevant reports on pharmacological activity and its mechanism research, etc.
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Embodiment 1
[0042] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:
[0043] Take 100g of total polysaccharides of Codonopsis pilosula, add 2000mL of distilled water, and dissolve at 80°C for 1 hour, stirring continuously during this period. Stand at 4°C for 12 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 90% ethanol, allowed to stand at 4°C for 12 hours, and then centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 3000mL distilled water at 80°C for 1 hour, stir continuously during this period, let it stand at 4°C for 12 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure was 1.5 atmospheres, and the filtrate that finally passed through the 5000 molecular weight membrane was concentrated and dried to obtain 36.45 g of CPS-A.
Embodiment 2
[0045] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:
[0046]Take 100g of Codonopsis total polysaccharides, add 3000mL of distilled water, and dissolve at 85°C for 1.5 hours, stirring continuously during this period. Stand at 4°C for 24 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 95% ethanol, left at 4°C for 24 hours, and centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 4000mL distilled water at 85°C for 1.5 hours, stir continuously during this period, let stand at 4°C for 24 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure is 2.0 atmospheric pressure, the filtrate that finally passes through the 5000 molecular weight membrane is concentrated and dried to obtain 40.30 g of CPS-A.
Embodiment 3
[0048] The preparation of Codonopsis polysaccharide CPS-A comprises the following steps:
[0049] Take 100g of Codonopsis total polysaccharides, add 2500mL of distilled water, and dissolve at 85°C for 1 hour, stirring continuously during this period. Stand at 4°C for 24 hours and centrifuge. The supernatant was concentrated to 100 mL, precipitated with 95% ethanol, left at 4°C for 24 hours, and centrifuged. After the precipitate is dried, repeat the previous operation to obtain the polysaccharide precipitate, dissolve it in 3500mL distilled water at 85°C for 1 hour, stir continuously during this period, let stand at 4°C for 24 hours, centrifuge, and ultrafilter the supernatant with a 100,000 molecular weight membrane and a 5,000 molecular weight membrane in turn , the pressure was 2.5 atmospheres, and the filtrate that finally passed through the 5000 molecular weight membrane was concentrated and dried to obtain 41.50 g of CPS-A.
[0050] The structural identification of Cod...
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