Rapid PVY virus identification method and application thereof

Abstract

The invention provides a rapid PVY virus identification method. A PVY virus is propagated in an identification host system and judged according to representations of different hosts in the identification host system; identification hosts in the identification host system comprise ordinary tobacco, nicotiana glutinosa, cucumbers or zucchini, gomphrena globosa, chenopodium amaranticolor and datura; the representations of the identification hosts after the PVY virus is propagated among the identification hosts are shown as follows: the ordinary tobacco has mosaic disease and necrosis; the nicotiana glutinosa has mosaic disease or necrosis; the cucumbers or zucchini are or are or is not infected; the gomphrena globose is not infected; the chenopodium amaranticolor has necrosis; the Datura is not infected. The method is simple, low in cost and reliable in result and can be popularized and used. The invention further relates to an application of the rapid PVY virus identification method in agricultural planting.

Description

technical field [0001] The invention relates to the field of agricultural detection, in particular to a PVY virus rapid identification method and application thereof. Background technique [0002] In agricultural planting, insect pests and diseases have always been the hazards of the planting industry, which are related to the quality and yield of agricultural products, and sometimes even cause the failure of agricultural products. Therefore, the prevention and control of insect pests and diseases has always been extremely important in agricultural planting. link, and pesticides are an effective way to solve this problem. However, with the deepening of the concept of environmental protection and the popularization of healthy food, people's demand for pollution-free and pesticide-free agricultural products continues to increase. According to reports of excessive residues, the damage of pesticide residues to human health is very serious. Therefore, it is necessary to strictly ...

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Problems solved by technology

[0004] At present, although the prevention and treatment of PVY virus is relatively mature, how to detect and identify PVY virus in time has become a key to the removal of PVY virus. The conventional identification of PVY virus includes serological identification methods, double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and antigen direct coating method, electron microscope method, etc., the above-mentioned identification methods require high technical and equipment requirements, or high cost, etc., which are not convenient for planters to use, and the general experience judgment is relatively rough , often erroneous judgments

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