Rapid PVY virus identification method and application thereof
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An identification method and virus technology, which is applied in the field of agricultural testing, can solve the problems of high cost, roughness, and wrong judgment, and achieve the effects of convenient identification, reliable results, and low cost
Inactive Publication Date: 2017-05-10
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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Problems solved by technology
[0004] At present, although the prevention and treatment of PVY virus is relatively mature, how to detect and identify PVY virus in time has become a key to the removal of PVY virus. The conventional identification of PVY virus includes serological identification methods, double antibody sandwich enzyme-linked immunosorbent ...
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Embodiment 1
[0015] Using the PVY virus rapid identification method of the present invention in tobacco planting
[0016] Tobacco is planted in the tobacco field, and several rows are planted at intervals in the tobacco ridge to identify hosts, including ordinary tobacco, heart leaf tobacco, cucumber or zucchini, amaranth, amaranth, and datura.
[0017] During the planting period, if mosaic leaves appear on the tobacco leaves, the tobacco leaves with more serious lesions are picked, then ground, and the ground juice is rubbed with the leaves to identify the host.
[0018] After friction, each of the following situations occurs:
[0019]
[0020] Then it can be judged that the infected virus is PVY virus.
[0021] Observe under the electron microscope, verify and determine that the infected virus is PVY virus.
Embodiment 2
[0023] The processing method is the same as the embodiment, as after the friction, the following situations occur respectively:
[0024]
[0025] Then it can be judged that the infected virus is not PVY virus, and it is confirmed as PVX virus by electron microscope.
[0026] The present invention starts from the traditional indication of plant lesions, and the creative expression is not only to select different indications of various hosts for different diseases to form a scientific and reasonable combination, but also to distinguish different diseases from each other, that is, the same Identifying the host system has different indication effects for different diseases and prevents duplication. It not only achieves the identification of diseases, but also distinguishes different diseases.
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Abstract
The invention provides a rapid PVY virus identification method. A PVY virus is propagated in an identification host system and judged according to representations of different hosts in the identification host system; identification hosts in the identification host system comprise ordinary tobacco, nicotiana glutinosa, cucumbers or zucchini, gomphrena globosa, chenopodium amaranticolor and datura; the representations of the identification hosts after the PVY virus is propagated among the identification hosts are shown as follows: the ordinary tobacco has mosaic disease and necrosis; the nicotiana glutinosa has mosaic disease or necrosis; the cucumbers or zucchini are or are or is not infected; the gomphrena globose is not infected; the chenopodium amaranticolor has necrosis; the Datura is not infected. The method is simple, low in cost and reliable in result and can be popularized and used. The invention further relates to an application of the rapid PVY virus identification method in agricultural planting.
Description
technical field [0001] The invention relates to the field of agricultural detection, in particular to a PVY virus rapid identification method and application thereof. Background technique [0002] In agricultural planting, insect pests and diseases have always been the hazards of the planting industry, which are related to the quality and yield of agricultural products, and sometimes even cause the failure of agricultural products. Therefore, the prevention and control of insect pests and diseases has always been extremely important in agricultural planting. link, and pesticides are an effective way to solve this problem. However, with the deepening of the concept of environmental protection and the popularization of healthy food, people's demand for pollution-free and pesticide-free agricultural products continues to increase. According to reports of excessive residues, the damage of pesticide residues to human health is very serious. Therefore, it is necessary to strictly ...
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