Molecular marker of pear PpAIV2 gene locus and screening method of molecular marker
A molecular marker and gene locus technology, applied in the field of molecular genetics, can solve problems such as difficulty and long cycle, and achieve the effects of high cost, convenient and rapid detection, and accelerated selection progress.
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Embodiment 1
[0027] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,
[0028] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;
[0029] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplification products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.06g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;
[0030] C. Analyze the genetic ...
Embodiment 2
[0035] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,
[0036] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;
[0037] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplified products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.08g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;
[0038]C. Analyze the genetic segre...
Embodiment 3
[0041] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,
[0042] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;
[0043] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplified products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.07g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;
[0044] C. Analyze the genetic segr...
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