Dionaea muscipula tissue culture and seedling raising method

Abstract

The invention discloses a dionaea muscipula tissue culture and seedling raising method. The method comprises the following steps: (1) preparing a culture medium; (2) preparing an explant; (3) performing the inducing culture; (4) rooting; and (5) performing bagging domestication. By adopting the method, the problems of the traditional sowing multiplication method and leaf cutting multiplication method that the success rate is low, the multiplication efficiency is low and the like can be solved; and when the method of the invention is adopted to perform the tissue culture and seedling raising for the dionaea muscipula, the multiplication coefficient of the culture medium reaches 4 to 5, a batch of culture media can be multiplied only in 4 to 6 months, so that the method has the characteristics of high multiplication coefficient and high multiplication speed; and moreover, the multiplied seedling has the same plant feature with a parent body and is good in resistance. The problem that the dionaea muscipula is difficult to multiply is solved, and the industrialized mass production of the dionaea muscipula can be facilitated.

Description

technical field [0001] The invention relates to the technical field of growing seedlings by plant tissue culture, in particular to a method for growing seedlings by tissue culture of Venus flytrap. Background technique [0002] Flytrap (Dionaea muscipula), the English name is Venus Flytrap, is a perennial herb native to North America, and is a very interesting carnivorous plant. As people's living standards improve day by day, more and more flower lovers begin to plant Flytraps. However, the Venus flytrap in its native place is threatened by human activities. The rapid increase in population has deprived Flytraps of their living space, and because of human intervention and natural wildfires, some small shrubs have begun to grow in these original areas, blocking the sunlight of Flytraps, causing some species of Flytraps to become endangered. [0003] The propagation methods of Venus flytrap are mainly seed propagation and leaf cutting propagation. [0004] The problem of a...

AI Technical Summary

Problems solved by technology

[0004] The problem of adopting seed propagation is: Flytraps can self-pollinate, but usually artificial pollination is required to produce fruit. Because the male and female stamens of Flytraps will not mature together, artificial pollination is difficult to succeed. The seeds of Flytraps are not It is resistant to storage, and the seeds are very small and less than one millimeter in diameter, so they are not easy to store and are easily damaged

[0005] The problems of using leaf cuttings for propagation are: the formation of new shoots is very slow when using leaf cuttings, and different varieties have different requirements for temperature, humidity, light, etc., so it is difficult to succeed in leaf cuttings

[0006] The traditional sowing propagation and leaf cutting propagation have the disadvantages of extremely low success rate and slow reproduction efficiency, which is difficult to meet the market demand of Venus flytrap

At present, there are very few studies on the reproduction of Flytraps, and there are few domestic enterprises that carry out factory production of Flytraps. factory production