A method for cultivating local excellent livestock breeds using somatic cell cloning technology

Abstract

The invention relates to a method for culturing a local excellent livestock variety by utilizing a somatic cell cloning technique. The method comprises the following steps: utilizing the somatic cell cloning technique to culture an excellent livestock variety; screening an individual with excellent economic characters from a livestock group so as to establish a fiber cell line; utilizing a somatic cell nuclear transfer technique to clone the screened individual; after the cloned individual grows to the adaptive age, performing combined hybridization; identifying the economic characters of each combined hybridized F1 generation individual; for the F1 generation individual parents which cannot reach the breeding standard, confirming whether the characters are resulted from the father generation or the mother generation; eliminating the corresponding individual parents; grouping the parents capable of executing stable inheritance in the left descendant individuals; and breeding the excellent livestock variety. According to the embodiment of the invention, livestock individuals with excellent economic characters can be obtained in batches, the sustainable development utilization for the peculiar variety resources of the livestock can be realized, and the breeding efficiency of the livestock and the breeding success rate can be promoted.

Description

technical field [0001] The invention relates to a method for cultivating local excellent livestock breeds by using somatic cell cloning technology, which belongs to the field of animal genetics, breeding and reproduction. Background technique [0002] The traditional breeding method of superior livestock breeds is mainly to select male and female individuals with excellent traits for continuous crossing, and repeatedly select the superior individuals in the offspring, so as to obtain target livestock individuals with excellent traits. For the very small number of exceptionally good individuals in the same breed of livestock or even the rare livestock individuals that are already facing extinction, it is difficult to achieve the selection of target individuals with excellent breeds through traditional breeding methods, and faced with the Insufficient resources, long breeding cycle, and low breeding success rate are not conducive to the industrialization of fine livestock indi...

AI Technical Summary

Problems solved by technology

However, the current sheep breeding method is mainly based on the traditional hybrid breeding model of "cross-crossing fixed-breeding improvement" (Tan Xiaoshan, Li Bing et al., 2015), and there are not many Erlang cashmere goats with good breeds They are scattered in different regions and different flocks, which restricts the pace of cross-breeding improvement to a certain extent.

At the same time, there are many times when these excellent varieties are discovered, most of the individuals are too old to be used for natural reproduction, and some have even died. If the traditional conservation methods are adopted, it is likely to lead to the eventual death of this excellent variety. die out

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